Genome sequence of Haemophilus parasuis strain 29755

Mullins, Michael A.; Register, Karen B.; Bayles, Darrell O.; Dyer, David W.; Kuehn, Joanna S.; Phillips, Gregory J.

doi:10.4056/sigs.2245029

Cited by 17 publications

(12 citation statements)

References 23 publications

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“…Analysis of the first complete H. parasuis genome sequence (strain SH0165) (36) identified a 14-kb polysaccharide biosynthesis region that was thought to encode an O antigen, with 12 predicted coding sequences (CDSs) in the same transcriptional direction. This locus was also found in the genome sequence of H. parasuis strain 29755 (37). The assignation of putative function to the predicted products of these genes was based on their similarity to other glycosyltransferases and polysaccharide processing/export proteins.…”

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confidence: 99%

Gene Content and Diversity of the Loci Encoding Biosynthesis of Capsular Polysaccharides of the 15 Serovar Reference Strains of Haemophilus parasuis

Howell

Weinert

Luan

et al. 2013

Journal of Bacteriology

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gHaemophilus parasuis is the causative agent of Glässer's disease, a systemic disease of pigs, and is also associated with pneumonia. H. parasuis can be classified into 15 different serovars. Here we report, from the 15 serotyping reference strains, the DNA sequences of the loci containing genes for the biosynthesis of the group 1 capsular polysaccharides, which are potential virulence factors of this bacterium. We contend that these loci contain genes for polysaccharide capsule structures, and not a lipopolysaccharide O antigen, supported by the fact that they contain genes such as wza, wzb, and wzc, which are associated with the export of polysaccharide capsules in the current capsule classification system. A conserved region at the 3= end of the locus, containing the wza, ptp, wzs, and iscR genes, is consistent with the characteristic export region 1 of the model group 1 capsule locus. A potential serovar-specific region (region 2) has been found by comparing the predicted coding sequences (CDSs) in all 15 loci for synteny and homology. The region is unique to each reference strain with the exception of those in serovars 5 and 12, which are identical in terms of gene content. The identification and characterization of this locus among the 15 serovars is the first step in understanding the genetic, molecular, and structural bases of serovar specificity in this poorly studied but important pathogen and opens up the possibility of developing an improved molecular serotyping system, which would greatly assist diagnosis and control of Glässer's disease.

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confidence: 99%

Gene Content and Diversity of the Loci Encoding Biosynthesis of Capsular Polysaccharides of the 15 Serovar Reference Strains of Haemophilus parasuis

Howell

Weinert

Luan

et al. 2013

Journal of Bacteriology

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“…The current commercially available vaccines are bacterins, which are protective only against strains of the same serovar (16)(17)(18), and which primarily target the disease-causing serovars 4 and 5, with limited cross-protection against others (5,19,20). It is possible to make autogenous vaccines in response to an outbreak of Glässer's disease, which can be useful if the serovar is different from that targeted by the commercial vaccines (21), but this is an expensive and time-consuming option.…”

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confidence: 99%

Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis

et al. 2015

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iHaemophilus parasuis causes Glässer's disease and pneumonia in pigs. Indirect hemagglutination (IHA) is typically used to serotype this bacterium, distinguishing 15 serovars with some nontypeable isolates. The capsule loci of the 15 reference strains have been annotated, and significant genetic variation was identified between serovars, with the exception of serovars 5 and 12. A capsule locus and in silico serovar were identified for all but two nontypeable isolates in our collection of >200 isolates. Here, we describe the development of a multiplex PCR, based on variation within the capsule loci of the 15 serovars of H. parasuis, for rapid molecular serotyping. The multiplex PCR (mPCR) distinguished between all previously described serovars except 5 and 12, which were detected by the same pair of primers. The detection limit of the mPCR was 4.29 ؋ 10 5 ng/l bacterial genomic DNA, and high specificity was indicated by the absence of reactivity against closely related commensal Pasteurellaceae and other bacterial pathogens of pigs. A subset of 150 isolates from a previously sequenced H. parasuis collection was used to validate the mPCR with 100% accuracy compared to the in silico results. In addition, the two in silico-nontypeable isolates were typeable using the mPCR. A further 84 isolates were analyzed by mPCR and compared to the IHA serotyping results with 90% concordance (excluding those that were nontypeable by IHA). The mPCR was faster, more sensitive, and more specific than IHA, enabling the differentiation of 14 of the 15 serovars of H. parasuis. Haemophilus parasuis is a Gram-negative bacterium commonly found in the upper respiratory tract of the pig, and it was identified in 1910 as the causative agent of a globally prevalent systemic disease of pigs known as Glässer's disease. The more severe presentations of this disease include arthritis, meningitis, polyserositis, septicemia, and pneumonia (1-5). Based on statistics from the United States, H. parasuis is the leading cause of mortality (alongside the porcine reproductive and respiratory syndrome [PRRS] virus) in nursery herds, and it is the third most important bacterial pathogen affecting finisher herds (6). H. parasuis also contributes to a multifactorial porcine respiratory disease complex, the leading cause of mortality in grower-finisher pigs in the United States (7). Diagnostic submissions to veterinary investigation centers of the Animal and Plant Health Agency (APHA) in 2013 and 2014 recorded the highest annual rates of diagnosis of disease incidents due to H. parasuis in England and Wales since 2002 (8, 9). In the third quarter of 2013, the diagnostic rate reached nearly 8% of diagnosable submissions (8,9). This disease characteristically manifests postweaning and is associated with the loss of maternally derived antibodies and the endemic presence of the bacterium in herds (1, 5).Treatment and prevention of Glässer's disease are implemented via strategic delivery of penicillin-based antimicrobials in feed or water. Ongoing treatment may be...

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“…Haemophilus parasuis is a commensal bacterium that inhabits the airways of pigs, also belongs to the family Pasteurellaceae and like another members of the same family, its growth requires V factor (nicotinamide adenine dinucleotide, NAD) but not X factor (hemin) [95][96][97]. H. parasuis is a Gram negative bacterium that causes Glässer's disease or poliserositis, can also cause septicemia, arthritis, meningitis and pneumonia [98], and under certain circumstances, could have the behavior of an opportunistic pathogen [96].…”

Section: Haemophilus Parasuismentioning

confidence: 99%

Porcine Respiratory Disease Complex and Biofilms

Loera‐Muro¹,

Ramírez-Castillo²,

Avelar-González³

et al. 2015

J Bacteriol Parasitol

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The Porcine respiratory disease complex (PRDC) is a term used to describe polymicrobial respiratory infections in pigs. Respiratory diseases in pigs are common in modern pork production worldwide and are the responsible for major economic losses in the swine industry. Pathogens involved in respiratory disease in pigs vary significantly among farms, production sites, regions and countries, making generalizations about the PRDC treatment and difficult to control it. The interactions that occur on the cellular and molecular levels during concurrent infection of pigs with two or more respiratory pathogens are multi-faceted and convoluted. There are a variety of bacterial and viral pathogens commonly associated with the PRDC. The main associated bacteria include: Actinobacillus pleuropneumoniae, Streptococcus suis, Pasteurella multocida, Bordetella bronchiseptica, Haemophilus pasaruis, and Mycoplasma hyopneumoniae. Currently, it is known among microbiologists that biofilm formation is an universal attribute of microorganisms and the main way of life in nature that are causing problems such as developing diseases in animals and humans. Here, is reviewed the current knowledge of the major bacteria involved in this disease, their ability to form biofilms, as well as their importance on the infection process.

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Genome sequence of Haemophilus parasuis strain 29755

Cited by 17 publications

References 23 publications

Gene Content and Diversity of the Loci Encoding Biosynthesis of Capsular Polysaccharides of the 15 Serovar Reference Strains of Haemophilus parasuis

Gene Content and Diversity of the Loci Encoding Biosynthesis of Capsular Polysaccharides of the 15 Serovar Reference Strains of Haemophilus parasuis

Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis

Porcine Respiratory Disease Complex and Biofilms

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