Genome Sequence of
            <i>Mycobacterium</i>
            Phage Waterfoul

Jackson, Paige N.; Embry, Ella K.; Johnson, Christa O.; Watson, Tiara L.; Weast, Sayre K.; DeGraw, Caroline J.; Douglas, Jessica R.; Sellers, J. Michael; D’Angelo, William; Mavrodi, Dmitri V.

doi:10.1128/genomea.01281-16

Cited by 1 publication

(2 citation statements)

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“…Here, we report the results of a genome-wide overexpression screen of the complete set of genes encoded by a single mycobacteriophage. Phage Waterfoul is a temperate siphovirus isolated on M. smegmatis mc 2 155 ( Jackson et al 2016 ). Based on gene content similarity, Waterfoul is part of phage cluster K ( Russell and Hatfull 2017 ), which includes several members that have been shown to infect clinically important strains of mycobacteria ( Pope et al 2011 ; Jacobs-Sera et al 2012 ; Dedrick et al 2019a , 2021 ; Guerrero-Bustamante et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Waterfoul encodes 94 predicted protein-coding genes and a single predicted tRNA(Trp) ( Fig. 1 ), and as is typical for phage genomes, most genes (∼60%) have no assigned functions ( Jackson et al 2016 ; Russell and Hatfull 2017 ). In our study, a diverse set of 32 Waterfoul genes were found to be cytotoxic when expressed from a multi-copy plasmid in the host bacterium M. smegmatis , including 17 NKF genes and several genes with predicted core viral functions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Systematic overexpression of genes encoded by mycobacteriophage Waterfoul reveals novel inhibitors of mycobacterial growth

Heller

Amaya

Mohamed

et al. 2022

G3 Genes|Genomes|Genetics

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Bacteriophages represent an enormous reservoir of novel genes, many of which are unrelated to existing entries in public databases and cannot be assigned a predicted function. Characterization of these genes can provide important insights into the intricacies of phage–host interactions and may offer new strategies to manipulate bacterial growth and behavior. Overexpression is a useful tool in the study of gene-mediated effects, and we describe here the construction of a plasmid-based overexpression library of a complete set of genes for Waterfoul, a mycobacteriophage closely related to those infecting clinically important strains of Mycobacterium tuberculosis and/or Mycobacterium abscessus. The arrayed Waterfoul gene library was systematically screened in a plate-based cytotoxicity assay, identifying a diverse set of 32 Waterfoul gene products capable of inhibiting the growth of the host Mycobacterium smegmatis and providing a first look at the frequency and distribution of cytotoxic products encoded within a single mycobacteriophage genome. Several of these Waterfoul gene products were observed to confer potent anti-mycobacterial effects, making them interesting candidates for follow-up mechanistic studies.

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