Genome Sequence of Jumbo Phage vB_AbaM_ME3 of
            <i>Acinetobacter baumanni</i>

Buttimer, Colin; O’Sullivan, L.; Elbreki, Mohamed; Neve, Horst; McAuliffe, Olivia; Ross, R. Paul; Hill, Colin; O’Mahony, Jim; Coffey, Aidan

doi:10.1128/genomea.00431-16

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…A number of jumbo phages like CBB have been reported in recent years (Kim et al, 2013; Šimolūnas et al, 2013; Abbasifar et al, 2014; Drulis-Kawa et al, 2014; Buttimer et al, 2016; Yuan and Gao, 2016). Among them, phage CBB is significant given the identification of atypical whisker-like structures on its contractile tail, for which a function has not yet been assigned.…”

Section: Resultsmentioning

confidence: 99%

Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB

et al. 2017

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Enterobacteria phage vB_PcaM_CBB is a “jumbo” phage belonging to the family Myoviridae. It possesses highly atypical whisker-like structures along the length of its contractile tail. It has a broad host range with the capability of infecting species of the genera Erwinia, Pectobacterium, and Cronobacter. With a genome of 355,922 bp, excluding a predicted terminal repeat of 22,456 bp, phage CBB is the third largest phage sequenced to date. Its genome was predicted to encode 554 ORFs with 33 tRNAs. Based on prediction and proteome analysis of the virions, 29% of its predicted ORFs could be functionally assigned. Protein comparison shows that CBB shares between 33–38% of its proteins with Cronobacter phage GAP32, coliphages PBECO4 and 121Q as well as Klebsiella phage vB_KleM_Rak2. This work presents a detailed and comparative analysis of vB_PcaM_CBB of a highly atypical jumbo myoviridae phage, contributing to a better understanding of phage diversity and biology.

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Section: Resultsmentioning

confidence: 99%

Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB

et al. 2017

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“…Phage vB_AbaM_ME3 was previously isolated from wastewater effluent using the propagating host Acinetobacter baumannii DSM 30007 (Kropinski et al, 2009 ). A. baumannii is a known nosocomial pathogen that causes pneumonia, urinary tract infection, and septicemia (Buttimer et al, 2016 ). Phage vB_AbaM_ME3 of A. baumannii has a size of 234,900 bp and 326 ORFs (Buttimer et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

“…A. baumannii is a known nosocomial pathogen that causes pneumonia, urinary tract infection, and septicemia (Buttimer et al, 2016 ). Phage vB_AbaM_ME3 of A. baumannii has a size of 234,900 bp and 326 ORFs (Buttimer et al, 2016 ). The Myovirus-type phage RSA1 is relatively small (39 to 40 kb) and has lytic activity.…”

Section: Resultsmentioning

confidence: 99%

Identification of Antimicrobial Resistance Determinants in Aeromonas veronii Strain MS-17-88 Recovered From Channel Catfish (Ictalurus punctatus)

Tekedar

Arick

Hsu

et al. 2020

Front. Cell. Infect. Microbiol.

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Aeromonas veronii is a Gram-negative species ubiquitous in different aquatic environments and capable of causing a variety of diseases to a broad host range. Aeromonas species have the capability to carry and acquire antimicrobial resistance (AMR) elements, and currently multi-drug resistant (MDR) Aeromonas isolates are commonly found across the world. A. veronii strain MS-17-88 is a MDR strain isolated from catfish in the southeastern United States. The present study was undertaken to uncover the mechanism of resistance in MDR A. veronii strain MS-17-88 through the detection of genomic features. To achieve this, genomic DNA was extracted, sequenced, and assembled. The A. veronii strain MS-17-88 genome comprised 5,178,226-bp with 58.6% G+C, and it encoded several AMR elements, including imiS, ampS, mcr-7.1, mcr-3, catB2, catB7, catB1, floR, vat(F), tet(34), tet(35), tet(E), dfrA3, and tetR. The phylogeny and resistance profile of a large collection of A. veronii strains, including MS-17-88, were evaluated. Phylogenetic analysis showed a close relationship between MS-17-88 and strain Ae5 isolated from fish in China and ARB3 strain isolated from pond water in Japan, indicating a common ancestor of these strains. Analysis of phage elements revealed 58 intact, 63 incomplete, and 15 questionable phage elements among the 53 A. veronii genomes. The average phage element number is 2.56 per genome, and strain MS-17-88 is one of two strains having the maximum number of identified prophage elements (6 elements each). The profile of resistance against various antibiotics across the 53 A. veronii genomes revealed the presence of tet(34), mcr-7.1, mcr-3, and dfrA3 in all genomes (100%). By comparison, sul1 and sul2 were detected in 7.5% and 1.8% of A. veronii genomes. Nearly 77% of strains carried tet(E), and 7.5% of strains carried floR. This result suggested a low abundance and prevalence of sulfonamide and florfenicol resistance genes compared with tetracycline resistance among A. veronii strains. Overall, the present study provides insights into the resistance patterns among 53 A. veronii genomes, which can inform therapeutic options for fish affected by A. veronii.

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“…Most of them were linear double-stranded DNA, of which 23 were circular DNA. Their genome sizes ranged from 4 [ 64 ]to 234 kb [ 65 ]. The GC content and host content ranged from 30.76% [ 65 ] to 47.11% [ 46 ], and 3% [ 66 ] to 85% [ 34 ], respectively.…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter baumannii Bacteriophage: Progress in Isolation, Genome Sequencing, Preclinical Research, and Clinical Application

Xiao

Huang

2023

Curr Microbiol

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Acinetobacter baumannii (A. baumannii) is a common nosocomial pathogen associated with serious clinical challenges owing to its rapidly increasing resistance to antibiotics. Due to their high host specificity and easy access to the natural environment, bacteriophages (phages) may serve as good antibacterial agents. Phage therapy has been successfully used to treat antibiotic-resistant A. baumannii infections. As a fundamental step before phage therapy, the characterization and sequencing of A. baumannii phages have been well studied. Until October 2022, 132 A. baumannii phages have been sequenced and studied, with their genomes ranging from 4 to 234 kb, and we summarize the characterized and sequenced A. baumannii phages. This review is a current and short overview that does not go into detail on the A. baumannii phages. In addition, preclinical studies and clinical applications of A. baumannii phages are also included.

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Genome Sequence of Jumbo Phage vB_AbaM_ME3 of Acinetobacter baumanni

Cited by 12 publications

References 15 publications

Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB

Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB

Identification of Antimicrobial Resistance Determinants in Aeromonas veronii Strain MS-17-88 Recovered From Channel Catfish (Ictalurus punctatus)

Acinetobacter baumannii Bacteriophage: Progress in Isolation, Genome Sequencing, Preclinical Research, and Clinical Application

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