Genome Sequence of the Pattern-Forming Social Bacterium Paenibacillus dendritiformis C454 Chiral Morphotype

Sirota-Madi, Alexandra; Olender, Tsviya; Helman, Yael; Brainis, Ina; Finkelshtein, Alin; Roth, Dalit; Hagai, Efrat; Leshkowitz, Dena; Brodsky, Leonid; Galatenko, Vladimir; Николаев, В. И.; Gutnick, David L.; Lancet, Doron; Ben‐Jacob, Eshel

doi:10.1128/jb.00158-12

Cited by 20 publications

(14 citation statements)

References 14 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paenibacillus dendritiformis (morphotype C; also known as the chiral morphotype) is a spore-forming, motile bacterial species (56). Each bacterium is an elongated (filamentous), rigid (seldom bends by collisions), rod-shaped cell, with a fixed thickness of ϳ1 m and a very broad length distribution of ϳ17 Ϯ 12 m. The bacteria were maintained at Ϫ80°C in Luria broth (LB) (Sigma, St. Louis, MO) with 25% (wt/vol) glycerol.…”

Section: Methodsmentioning

confidence: 99%

Periodic Reversals in Paenibacillus dendritiformis Swarming

et al. 2013

Self Cite

View full text Add to dashboard Cite

Bacterial swarming is a type of motility characterized by a rapid and collective migration of bacteria on surfaces. Most swarming species form densely packed dynamic clusters in the form of whirls and jets, in which hundreds of rod-shaped rigid cells move in circular and straight patterns, respectively. Recent studies have suggested that short-range steric interactions may dominate hydrodynamic interactions and that geometrical factors, such as a cell's aspect ratio, play an important role in bacterial swarming. Typically, the aspect ratio for most swarming species is only up to 5, and a detailed understanding of the role of much larger aspect ratios remains an open challenge. Here we study the dynamics of Paenibacillus dendritiformis C morphotype, a very long, hyperflagellated, straight (rigid), rod-shaped bacterium with an aspect ratio of ϳ20. We find that instead of swarming in whirls and jets as observed in most species, including the shorter T morphotype of P. dendritiformis, the C morphotype moves in densely packed straight but thin long lines. Within these lines, all bacteria show periodic reversals, with a typical reversal time of 20 s, which is independent of their neighbors, the initial nutrient level, agar rigidity, surfactant addition, humidity level, temperature, nutrient chemotaxis, oxygen level, illumination intensity or gradient, and cell length. The evolutionary advantage of this unique back-and-forth surface translocation remains unclear. Motile bacteria are able to colonize surfaces using various motility mechanisms (1). One efficient method includes flagellation-based cell motion in conjunction with collective lubrication (typically by secretion of surfactants) to enable fast expansion on hard surfaces. This mode of "bacterial swarming" that has been studied extensively for many species (1-15) enables rapid colony expansion (up to centimeters per hour). Swarming is often marked by hundreds of cells moving in a coordinated fashion while generating whirl and jet patterns.Studies of the collective dynamics of swarming have examined multiple aspects of motility. On the macroscopic level, it was discovered that swarming colonies show an advantage over liquid cultures in that they exhibit an increased resistance to antimicrobials (1,4,5,(15)(16)(17)(18)(19)(20). Studies of collective secretions of signaling and quorum-sensing molecules have shown how interactions between cells in swarming colonies are controlled (11) and exposed the identification of associated genetic manipulations and upregulated proteins that control biosurfactant secretions and flagellar behavior. On the single-cell level, attention was given to swarm cell trajectories and the ways in which these trajectories are determined by flagellar motion (8,(21)(22)(23). A combination of experiments (2, 3, 14, 15, 24-38) and theory (39-43) suggests that hydrodynamic interactions play a significant role in this social form of migration.Hydrodynamic interactions may not always be the dominant physical mechanism controlling bacterial motion....

show abstract

Section: Methodsmentioning

confidence: 99%

Periodic Reversals in Paenibacillus dendritiformis Swarming

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Paenibacillus dendritiformis is an interesting bacterial species marked by the complex spatial organization of its colony, which can form different patterns of growth on semi-solid agar such as chiral branches, swirls, and vortices [67,68]. It was observed that the sibling P. dendritiformis bacterial colonies grown on low-nutrient agar medium mutually inhibited the growth of each other [69].…”

Section: "Forced Suicide" In Paenibacillus Dendritiformismentioning

confidence: 99%

Harnessing the Potential of Killers and Altruists within the Microbial Community: A Possible Alternative to Antibiotic Therapy?

et al. 2019

View full text Add to dashboard Cite

In the context of a post-antibiotic era, the phenomenon of microbial allolysis, which is defined as the partial killing of bacterial population induced by other cells of the same species, may take on greater significance. This phenomenon was revealed in some bacterial species such as Streptococcus pneumoniae and Bacillus subtilis, and has been suspected to occur in some other species or genera, such as enterococci. The mechanisms of this phenomenon, as well as its role in the life of microbial populations still form part of ongoing research. Herein, we describe recent developments in allolysis in the context of its practical benefits as a form of cell death that may give rise to developing new strategies for manipulating the life and death of bacterial communities. We highlight how such findings may be viewed with importance and potential within the fields of medicine, biotechnology, and pharmacology.

show abstract

“…Moderately thermophilic E. profundum was defined by Crapart 15 but its industrial enzyme abilities never examined while lipase production of E. profundum discovered in this study. P. dendritiformis, which was included in the genus Bacillus, was first discovered in 1990s and reclassified as a separate genus 49 . However, there is no record about P. dendritiformis from thermophilic sources while protease production was determined in this study.…”

Section: Enzyme Production Capacities Of Thermophilesmentioning

confidence: 99%

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Albayrak¹,

Genç²,

Özkan³

et al. 2019

J Pure Appl Microbiol

View full text Add to dashboard Cite

In this study, one hundred and thirty isolates were isolated from water and sludge samples taken from hot springs located in different regions of Turkey. Among them, eleven isolates were chosen according to conventional (morphological, physiological and biochemical tests) and molecular methods (rep-PCR and 16S rRNA sequencing). These bacteria were then tested for their capability to produce valuable enzymes. As a result; species belonging to Bacillus, Anoxybacillus, Aeribacillus, Enterococcus, Exiguobacterium and Paenibacillus were identified. Test strains were found to have optimum reproductive potential at pH 5.0-9.0 and 15-65°C, usually at a concentration of 1.0-10.0% (w/v) NaCl. In addition, all thermotolerant bacteria were Gram, endospore (except E. profundum), catalase and oxidase (except E. faecium and E. profundum) positive, and rod-shaped (except E. faecium). It was observed that all isolates had a 99% similarity percentage as a result of 16S rRNA sequence analysis. All of the isolates were capable of producing industrially important enzymes moreover, eight of them could produce at least two of these enzymes. Test strains had high potential of industrial enzyme production, and the enzymes from these thermo-tolerant isolates will be widely used in biotechnological processes.

show abstract

Genome Sequence of the Pattern-Forming Social Bacterium Paenibacillus dendritiformis C454 Chiral Morphotype

Cited by 20 publications

References 14 publications

Periodic Reversals in Paenibacillus dendritiformis Swarming

Periodic Reversals in Paenibacillus dendritiformis Swarming

Harnessing the Potential of Killers and Altruists within the Microbial Community: A Possible Alternative to Antibiotic Therapy?

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Contact Info

Product

Resources

About