Genome Sequence of Ureaplasma diversum Strain ATCC 49782

Marques, Lucas Miranda; Guimarães, Ana Márcia Sá; Martins, Hellen Braga; Rezende, Izadora S.; Barbosa, Maysa Santos; Campos, Guilherme Barreto; Nascimento, Naíla Cannes do; Santos, Andrea Pires dos; Amorim, Aline Teixeira; Santos, Vilmara Almeida dos; Messick, Joanne B.; Timenetsky, Jorge

doi:10.1128/genomea.00314-15

Cited by 10 publications

(15 citation statements)

References 8 publications

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“…NF-κB and AP-1 regulate important genes associated with inflammation in infection processes ( Garcia et al, 1998 ; Rawadi et al, 1999 ). Accordingly, the recent genome analyses of U. diversum revealed a large number of genes encoding membrane-associated lipoproteins, as well as urease, hemolysin, phospholipase and glycosyltransferase (associated with capsule synthesis) enzymes ( Marques et al, 2015 , 2016 ). These lipoproteins, however, have never been separately analyzed for their host immune system stimulation.…”

Section: Introductionmentioning

confidence: 99%

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

et al. 2018

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Objectives: Ureaplasma diversum is a pathogen of cows that may cause intense inflammatory responses in the reproductive tract and interfere with bovine reproduction. The aims of this study were to evaluate the immune response of bovine blastocysts and macrophages to U. diversum infection and to evaluate the invasion capacity of this microorganism in bovine blastocysts.Methods: Viable and heat-inactivated U. diversum strains ATCC 49782 and CI-GOTA and their extracted membrane lipoproteins were inoculated in macrophages in the presence or absence of signaling blockers of Toll-Like Receptor (TLR) 4, TLR2/4, and Nuclear Factor KB (NF-κB). In addition, the same viable U. diversum strains were used to infect bovine blastocysts. RNA was extracted from infected and lipoprotein-exposed macrophages and infected blastocysts and assayed by qPCR to evaluate the expression of Interleukin 1 beta (IL-1β), Tumor Necrosis Factor Alpha (TNF-α), TLR2 and TLR4 genes. U. diversum internalization in blastocysts was followed by confocal microscopy.Results: Both Ureaplasma strains and different concentrations of extracted lipoproteins induced a higher gene expression of IL-1β, TNF-α, TLR2, and TLR4 in macrophages (p < 0.05) when compared to non-infected cells. The used blockers inhibited the expression of IL-1β and TNF-α in all treatments. Moreover, U. diversum was able to internalize within blastocysts and induce a higher gene expression of IL-1b and TNF- α when compared to non-infected blastocysts (p < 0.05).Conclusion: The obtained results strongly suggest that U. diversum and its lipoproteins interact with TLR4 in a signaling pathway acting via NF-kB signaling to stimulate the inflammatory response. This is the first study to evaluate the in vitro immunological response of macrophages and bovine blastocysts against U. diversum. These results may contribute to a better understanding of the immunomodulatory activity and pathogenicity of this infectious agent.

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Section: Introductionmentioning

confidence: 99%

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

et al. 2018

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“…Asymptomatic cows may chronically carry U. diversum and function as pathogen reservoirs and are, therefore, a major source of infection. Bovine ureaplasmas have surface compounds, such as variable surface lipoproteins (VSPs) that allow them to evade the host's immune system, invade cells, and help develop chronic infection ( 28 , 42 , 88 – 90 ).…”

Section: Ureaplasma Diversummentioning

confidence: 99%

“…There is no data in the literature on the pathogenic effects of the expression of the urease gene by U. diversum ; however, in these bacteria, the urease genomic cluster was identified, with more than 90% homology to the human Ureaplasma spp. coding sequence (CDS) ( 28 , 90 ). Hydrolysis of urea in ureaplasma generates an electrochemical gradient through accumulation of intracellular ammonia/ammonium.…”

Section: Virulence Of U Diversummentioning

confidence: 99%

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A Review of Ureaplasma diversum: A Representative of the Mollicute Class Associated With Reproductive and Respiratory Disorders in Cattle

et al. 2021

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The Mollicutes class encompasses wall-less microbes with a reduced genome. They may infect plants, insects, humans, and animals including those on farms and in livestock. Ureaplasma diversum is a mollicute associated with decreased reproduction mainly in the conception rate in cattle, as well as weight loss and decreased quality in milk production. Therefore, U. diversum infection contributes to important economic losses, mainly in large cattle-producing countries such as the United States, China, Brazil, and India. The characteristics of Mollicutes, virulence, and pathogenic variations make it difficult to control their infections. Genomic analysis, prevalence studies, and immunomodulation assays help better understand the pathogenesis of bovine ureaplasma. Here we present the main features of transmission, virulence, immune response, and pathogenesis of U. diversum in bovines.

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“…These antigens are considered the main molecular agents associated with pathogens in several Mollicutes species and play an important role in host pathogenicity and immunomodulation [7]. In addition to lipoproteins, in the bovine ureaplasma genome, our research group identified genes encoding the multiple band antigen (MBA), which contain multiple series repetitions in the C-terminal region, as well as the gene for hemolysin and for the Mycoplasma Ig binding protein (MIB) and Mycoplasma Ig protease (MIP) -MIB-MIP system-, which acts by binding and cleaving the IgG heavy chain [2,8].…”

Section: Introductionmentioning

confidence: 99%

Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states

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et al. 2020

BMC Vet Res

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Background Ureaplasma diversum has numerous virulence factors that contribute to pathogenesis in cattle, including Lipid-associated membrane proteins (LAMPs). Therefore, the objectives of this study were to evaluate in silico important characteristics for immunobiological applications and for heterologous expression of 36 LAMPs of U. diversum (UdLAMPs) and, also, to verify by conventional PCR the distribution of these antigens in strains of Brazilian states (Bahia, Minas Gerais, São Paulo, and Mato Grosso do Sul). The Manatee database was used to obtain the gene and peptide sequences of the antigens. Similarity and identity studies were performed using BLASTp and direct antigenicity was evaluated by the VaxiJen v2.0 server. Epitope prediction for B lymphocytes was performed on the BepiPred v2.0 and CBTOPE v1.0 servers. NetBoLApan v1.0 was used to predict CD8+ T lymphocyte epitopes. Subcellular location and presence of transmembrane regions were verified by the software PSORTb v3.0.2 and TMHMM v2.2 respectively. SignalP v5.0, SecretomeP v2.0, and DOLOP servers were used to predict the extracellular excretion signal. Physico-chemical properties were evaluated by the web-software ProtParam, Solpro, and Protein-sol. Results In silico analysis revealed that many UdLAMPs have desirable properties for immunobiological applications and heterologous expression. The proteins gudiv_61, gudiv_103, gudiv_517, and gudiv_681 were most promising. Strains from the 4 states were PCR positive for antigens predicted with immunogenic and/or with good characteristics for expression in a heterologous system. Conclusion These works contribute to a better understanding of the immunobiological properties of the UdLAMPs and provide a profile of the distribution of these antigens in different Brazilian states.

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Genome Sequence of Ureaplasma diversum Strain ATCC 49782

Cited by 10 publications

References 8 publications

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

A Review of Ureaplasma diversum: A Representative of the Mollicute Class Associated With Reproductive and Respiratory Disorders in Cattle

Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states

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