Genome Sequence Resource for Stagonosporopsis cucurbitacearum, a Cause of Gummy Stem Blight Disease of Watermelon

Abstract

Gummy stem blight (GSB), which is caused by three related species of Stagonosporopsis, is a worldwide devastating disease of cucurbit crops including watermelon. Previously S. cucurbitacearum was reported to be the major fungal cause of watermelon GSB in Southern China, where it causes a significant decrease in watermelon yield. Here, we present the draft whole genome sequence, gene prediction and annotation of S. cucurbitacearum strain DBTL4, isolated from diseased watermelon plants. To our knowledge, this is the first publicly available genome sequence of this species, and knowledge of this genome sequence will help further understand the pathogenic mechanism of S. cucurbitacearum to cucurbit plants.

Gummy stem blight: One disease, three pathogens

“…More recently, the genomes of S. cucurbitacearum strains Zq‐1 (Zhao et al, 2020) and DBTL‐4 (Wang et al, 2021) were analysed. After high‐throughput genome sequencing, assembly, and filtering out any low‐quality reads for S. cucurbitacearum strain Zq‐1, a 35.28‐Mb genome sequence was obtained.…”

“…The genome of S. cucurbitacearum strain DBTL4, made available by Wang et al (2021), harbours 10,748 predicted protein‐coding genes, of which 812 encode secreted proteins. In the secretome, 113 proteins were reported as potential effectors that play important roles in deactivating host defence.…”

“…Moreover, 635 CAZymes, 2273 transmembrane proteins, 143 transport proteins, and 3101 PHI proteins were identified. Comparing the assembly of strain DBTL4 (Zhao et al, 2020) with that of strain Zq‐1 revealed that the assembly of Zq‐1 in terms of number of scaffolds and scaffold N 50 was better (Wang et al, 2021).…”

“…More recently, the genomes of S. cucurbitacearum strains Zq-1 (Zhao et al, 2020) and DBTL-4 (Wang et al, 2021) were analysed.…”

Potential Action Mechanism and Inhibition Efficacy of Morinda citrifolia Essential Oil and Octanoic Acid against Stagonosporopsis cucurbitacearum Infestations

“…Amino acid sequences of tyrosine-tRNA ligase from S. cucurbitacearum were obtained from previous investigations [62]. The protein 3D structure was constructed using a homology modeling approach with the Swiss Model Workspace (accessed on 13 April 2022 at https://swissmodel.expasy.org/) after the selection of its respective templates using the Protein Basic Local Align Search Tool.…”