Genome Sequences of RIGVIR Oncolytic Virotherapy Virus and Five Other Echovirus 7 Isolates

Abstract

We report here the nearly complete Illumina-sequenced consensus genome sequences of six isolates of echovirus 7 (E7), including oncolytic virotherapy virus RIGVIR and the Wallace prototype. Amino acid identities within the coding region were highly conserved across all isolates, ranging from 95.31% to 99.73%.

Cytolytic Properties and Genome Analysis of Rigvir® Oncolytic Virotherapy Virus and Other Echovirus 7 Isolates

“…We have recently genome-sequenced Rigvir ® and other E7 isolates [ 27 ]. Preliminary genome analysis suggested both similarities and differences between the isolates.…”

“…To further analyze the sequence data in respect to Rigvir ® ’s claimed properties, we performed sequence and phylogenetic analysis utilizing all full-length E7 sequence data available in GenBank. At present, there are 32 full-length E7 genome sequences in the GenBank (as of 6 December 2021), including our previously published 6 sequences [ 27 ]. All non-redundant ( n = 30) full-length sequences were aligned using Geneious Prime software and the MUSCLE [ 29 ] and MAFFT [ 30 ] alignment plugins within the program.…”

“…no. MH043132-MH043137) [ 27 ]. Data are also shown for the 24 full length E7 isolates obtained from GenBank for this study, including the prototype, Wallace [ 28 ].…”

“…2022, 14, x FOR PEER REVIEW 5 of 15 6 December 2021), including our previously published 6 sequences [27]. All non-redundant (n = 30) full-length sequences were aligned using Geneious Prime software and the MUSCLE [29] and MAFFT [30] alignment plugins within the program.…”

Comment on Hietanen et al. Cytolytic Properties and Genome Analysis of Rigvir® Oncolytic Virotherapy Virus and Other Echovirus 7 Isolates. Viruses 2022, 14, 525

“…In a recent article published in Viruses by Hietanen et al [1], the authors performed a phylogenetic analysis of the full-length nucleotide sequences of six different ECHO-7 virus isolates, some of which had been previously published by the same authors [2]. In addition, the authors tested for cytopathic effect on cells in culture, which partially repeated and extended previous results [3].…”