Genome Sequences of Three Frequently Used Listeria monocytogenes and Listeria ivanovii Strains

Klumpp, Jochen; Staubli, Titu; Schmitter, Sibylle; Hupfeld, Mario; Fouts, Derrick E.; Loessner, Martin J.

doi:10.1128/genomea.00404-14

Cited by 9 publications

(13 citation statements)

References 13 publications

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“…Phage_Finder ( 6 ) was used to screen the genomes for the presence of intact and cryptic phages. As previously reported for L. ivanovii WSLC 3009 ( 2 ), both L. ivanovii subsp. londoniensis WSLC 30167 and L. ivanovii subsp.…”

Section: Genome Announcementsupporting

confidence: 86%

“…The CRISPRFinder algorithm ( 4 ) was used to identify putative CRISPR loci within these strains. WSLC 3010 harbors three CRISPR regions, which are identical to those reported for WSLC 3009 ( 2 ). Two and three CRISPR regions were identified in WSLC 30151 and WSLC 30167, respectively.…”

Section: Genome Announcementsupporting

confidence: 63%

“… L. ivanovii WSLC 3010 is a widely used serovar 5 isolate that is quite similar to the recently sequenced WSLC 3009 strain ( 2 ). While strains WSLC 30151 and WSLC 30167 also belong to the serovar 5 group, they have been designated L. ivanovii subsp.…”

Section: Genome Announcementmentioning

confidence: 83%

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Genome Sequences of the Listeria ivanovii subsp. ivanovii Type Strain and Two Listeria ivanovii subsp. londoniensis Strains

et al. 2015

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Section: Genome Announcementsupporting

confidence: 86%

Section: Genome Announcementsupporting

confidence: 63%

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Genome Sequences of the Listeria ivanovii subsp. ivanovii Type Strain and Two Listeria ivanovii subsp. londoniensis Strains

et al. 2015

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“…These phenotypes suggest that all HMW PBPs contribute to listerial peptidoglycan biosynthesis; however, it is likely that this is achieved via their distinct as well as overlapping physiological functions. Interestingly, PBP A1 genes of L. monocytogenes isolates J0161 (reference number: NC_017545.1), J1816 (NC_021829.1) and WSLC1042 (CP007210.1) have acquired premature stop codons leading to truncated proteins lacking up to 110 C-terminal amino acids (Klumpp et al ., 2014 ). These truncations do, however, not extend into the transpeptidase domains and, hence, might be without functional consequences.…”

Section: Introductionmentioning

confidence: 99%

Discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of Listeria monocytogenes

Rismondo

Møller

Aldridge

et al. 2014

Molecular Microbiology

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Upon ingestion of contaminated food, Listeria monocytogenes can cause serious infections in humans that are normally treated with β-lactam antibiotics. These target Listeria's five high molecular weight penicillin-binding proteins (HMW PBPs), which are required for peptidoglycan biosynthesis. The two bi-functional class A HMW PBPs PBP A1 and PBP A2 have transglycosylase and transpeptidase domains catalyzing glycan chain polymerization and peptide cross-linking, respectively, whereas the three class B HMW PBPs B1, B2 and B3 are monofunctional transpeptidases. The precise roles of these PBPs in the cell cycle are unknown. Here we show that green fluorescent protein (GFP)-PBP fusions localized either at the septum, the lateral wall or both, suggesting distinct and overlapping functions. Genetic data confirmed this view: PBP A1 and PBP A2 could not be inactivated simultaneously, and a conditional double mutant strain is largely inducer dependent. PBP B1 is required for rod-shape and PBP B2 for cross-wall biosynthesis and viability, whereas PBP B3 is dispensable for growth and cell division. PBP B1 depletion dramatically increased β-lactam susceptibilities and stimulated spontaneous autolysis but had no effect on peptidoglycan cross-linkage. Our in vitro virulence assays indicated that the complete set of all HMW PBPs is required for maximal virulence.

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“…Although lysogeny in Listeria strains is very common (Loessner and Rees ), prophage genes are unevenly distributed among serotypes. Some strains are completely free or do not have complete prophages such as WSCL 1001 and WSCL 1042 (Klumpp and Loessner, ; Klumpp and others ), as well as F2365 (Nelson and others ), L312 (Chatterjee and others ), and SLCC 2376 (Haase and others ) as found by Kuenne and others (). These particular strains belong to serotypes 1/2a, 4b, and 4c.…”

Section: Monocytogenes In the Food Industrymentioning

confidence: 70%

Listeria monocytogenes: A Target for Bacteriophage Biocontrol

Strydom

Witthuhn

2015

Comp Rev Food Sci Food Safe

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Listeria monocytogenes is a growing concern in the food industry as it is the causative agent of human listeriosis. There are many research articles concerning the growth, survival, and diversity of L. monocytogenes strains isolated from food-related sources, elucidating the difficulty in controlling these bacteria in a food-processing facility. Bacteriophage biocontrol of L. monocytogenes strains was introduced in 2006, through the first commercial bacteriophage product targeting L. monocytogenes ListShield TM . This review focuses on the use of bacteriophage biocontrol to target L. monocytogenes in the food industry, specifically direct application of the bacteriophages to food products. In addition, we discuss characteristics of these bacteria that will have a significant influence on the effective treatment of bacteriophages such as genetic diversity between strains prevalent in one facility. There are many positive results of phage treatments targeting L. monocytogenes in food; however, success of in vitro studies might not be reproducible in practice. Future studies should focus on creating experimental design that will imitate the conditions found in the food industry, such as a stressed state of the targeted bacteria. In situ evaluation of bacteriophage treatment of L. monocytogenes will also be necessary because the presence of these bacteria in a processing facility can vary greatly regarding genetic diversity. The potential use of phages in the food-processing facility as a biosanitizer for L. monocytogenes, as well as the use of lysins to target these bacteria should also be explored. Despite the exciting research avenues that have to be explored, current research shows that biocontrol of L. monocytogenes is feasible and has potential to positively impact the food industry.

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Genome Sequences of Three Frequently Used Listeria monocytogenes and Listeria ivanovii Strains

Cited by 9 publications

References 13 publications

Genome Sequences of the Listeria ivanovii subsp. ivanovii Type Strain and Two Listeria ivanovii subsp. londoniensis Strains

Genome Sequences of the Listeria ivanovii subsp. ivanovii Type Strain and Two Listeria ivanovii subsp. londoniensis Strains

Discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of Listeria monocytogenes

Listeria monocytogenes: A Target for Bacteriophage Biocontrol

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