Genome sequencing analysis of Brazilian chicken anemia virus isolates that lack MSB-1 cell culture tropism

Nogueira, Eliana Ottati; Ferreira, Antônio José Piantino; Soares, Rodrigo Martins; Durigon, Edison Luiz; Lazzarin, Simaia; Brentano, Liana

doi:10.1016/j.cimid.2006.11.001

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…The VP1 gene had the highest variability similar to what previous studies have shown [24,25]. The minimum and the maximum genetic distances at nucleotide level between IR CAV and other strains were 0.9% (CAV-B/Chicken/ India) and 5.2% (CAU-269/7 Australia), respectively.…”

Section: Resultssupporting

confidence: 65%

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Full-length infectious clone of an Iranian isolate of chicken anemia virus

2016

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An Iranian field strain of chicken anemia virus (CAV), designated IR CAV, was isolated in the Marek's disease virus-transformed lymphoblastoid cell line MDCC-MSB1 (MSB1) culture for the first time. The full-length CAV DNA of this strain was cloned in the bacterial plasmid pTZ57R/T to create the molecular clone pTZ-CAV. The nucleotide and deduced amino acid sequences of viral proteins of IR CAV were compared with those of representative CAV sequences including reference and commercial vaccine strains. IR CAV was not related to vaccine strains and also found to have glutamine at positions 139 and 144 confirming previous studies in which such mutations were associated with a slow rate of virus spread in cell culture. pTZ-CAV was digested with PstI to release IR CAV DNA and then transfected into MSB1 cell by electroporation. The transfected cells showed cytopathic effect similar to virion-initiated infection. One-day old specific pathogen-free chicks were inoculated with the regenerated virus, which had been obtained from transfected MSB1 cells, and compared with the chicks inoculated with IR CAV. Gross lesions in the birds inoculated with the regenerated virus illustrated the infectious nature of the regenerated virus from the cloned IR CAV DNA.

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Section: Resultssupporting

confidence: 65%

“…Our findings confirm previous studies since we found that IR CAV had glutamine at positions 139 and 144 and also a slow rate of spread in cell culture. However, Nogueira et al [25] reported that a specific amino acid substitution alone in VP1, VP2, and VP3 may not be sufficient to account for CAV isolation and propagation in cell culture.…”

Section: Resultsmentioning

confidence: 99%

Full-length infectious clone of an Iranian isolate of chicken anemia virus

2016

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“…In the case of predicted amino acid sequences, minor differences have been noted particularly in the VP1 amino acid sites 139-151. The observed differences in the hypervariable region had impact on the predicted protein structure of VP1 [48][49][50]. In this study, the phylogenetic analysis of VP1 embodied the diversity of CAV, while the VP2 and VP3 embodied the conservatism.…”

Section: Discussionmentioning

confidence: 71%

Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015

et al. 2019

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Chicken anaemia virus (CAV), a member of the genus Gyrovirus, is the etiological agent of chicken infectious anaemia. CAV infects bone marrow-derived cells, resulting in severe anaemia and immunosuppression in young chickens and a compromised immune response in older birds. We investigated the molecular epidemiology of CAV in sick chickens in China from 2014 to 2015 and showed that the CAV-positive rate was 13.30%, in which mixed infection (55.56%) was the main type of infection. We isolated and identified 15 new CAV strains using different methods including indirect immunofluorescence assay and Western Blotting. We used overlapping polymerase chain reaction to map the whole genome of the strains. Phylogenetic analyses of the obtained sequences and related sequences available in GenBank generated four distinct groups (A–D). We built phylogenetic trees using predicted viral protein (VP) sequences. Unlike CAV VP2s and VP3s that were well conserved, the diversity of VP1s indicated that the new strains were virulent. Our epidemiological study provided new insights into the prevalence of CAV in clinical settings in recent years in China.

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“…MSB1 culture eventually exhibits a cytopathic effect (CPE) characterized by the appearance of enlarged cells, cell destruction, alkaline medium and loss of the ability to grow [2,6,11,25]. Although most CAV strains could be propagated on MSB1, recent isolates that lack the ability to infect and propagate on MSB1 have been reported in America [2,15,20], Bangladesh [8] and Brazil [14]. Moreover, some researchers have found that isolation of CAV using MSB1 may require from 10 to 16 passages [21] before the CPE becomes evident.…”

mentioning

confidence: 99%

“…Although MSB1 has been widely used for CAV isolation and propagation, some biological problems have been reported such as no support for the propagation of some recently isolated strains, i.e., 59058 and TR20 [20], BD3 [8] and BR47/90 [14]. MSB1 may lose its ability to support CAV propagation after a longer passage [22].…”

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confidence: 99%

Comparative Susceptibility of Some Avian and Mammalian Lymphoblastoid Cell Lines to Chicken Anemia Virus

Haridy

Goryo

Sasaki

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. Although MSB1 is the most-commonly used lymphoblastoid cell line for isolation of chicken anemia virus (CAV), some researchers have reported a few biological drawbacks. None of them were supported by the results of the present study. Another four avian (HP1, HP2, BK3 and CU10) and two mammalian (BTL-26 and KO-1) cell lines were investigated for susceptibility to the TK-5803 and AH-9409 strains. Both strains caused CPE on BK3 like MSB1. The mean number of positive cells for each strain in MSB1 and BK3 were not significantly different. The majority of the HP2, CU10 and HP1 cells showed no CPE. The virus titers of both strains were higher in MSB1 and BK3 (10 6.5-7.5 TCID 50 /0.1 ml) than in HP2, CU10 and HP1 (10 3.5-4.5 TCID 50 /0.1 ml). BTL-26 and KO-1 were resistant to CAV. BK3 could be used for isolation of CAV.

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Genome sequencing analysis of Brazilian chicken anemia virus isolates that lack MSB-1 cell culture tropism

Cited by 10 publications

References 32 publications

Full-length infectious clone of an Iranian isolate of chicken anemia virus

Full-length infectious clone of an Iranian isolate of chicken anemia virus

Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015

Comparative Susceptibility of Some Avian and Mammalian Lymphoblastoid Cell Lines to Chicken Anemia Virus

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