Genome sequencing and annotation of Serratia sp. strain TEL

Lephoto, Tiisetso E.; Gray, Vincent M.

doi:10.1016/j.gdata.2015.08.010

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Mosquito-associated S. marcescens show distinctly different features from those isolated from environmental (free-living) or clinical settings (Lauzon et al, 2003 ; Iguchi et al, 2014 ; Lephoto and Gray, 2015 ). For example, our findings show that S. marcescens ano1 and ano2 lack genes encoding prodigiosins which are typically produced by many Serratia spp.…”

Section: Discussionmentioning

confidence: 98%

Genomic, Physiologic, and Symbiotic Characterization of Serratia marcescens Strains Isolated from the Mosquito Anopheles stephensi

2017

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Strains of Serratia marcescens, originally isolated from the gut lumen of adult female Anopheles stephensi mosquitoes, established persistent infection at high rates in adult A. stephensi whether fed to larvae or in the sugar meal to adults. By contrast, the congener S. fonticola originating from Aedes triseriatus had lower infection in A. stephensi, suggesting co-adaptation of Serratia strains in different species of host mosquitoes. Coinfection at high infection rate in adult A. stephensi resulted after feeding S. marcescens and Elizabethkingia anophelis in the sugar meal, but when fed together to larvae, infection rates with E. anophelis were much higher than were S. marcescens in adult A. stephensi, suggesting a suppression effect of coinfection across life stages. A primary isolate of S. marcescens was resistant to all tested antibiotics, showed high survival in the mosquito gut, and produced alpha-hemolysins which contributed to lysis of erythrocytes ingested with the blood meal. Genomes of two primary isolates from A. stephensi, designated S. marcescens ano1 and ano2, were sequenced and compared to other Serratia symbionts associated with insects, nematodes and plants. Serratia marcescens ano1 and ano2 had predicted virulence factors possibly involved in attacking parasites and/or causing opportunistic infection in mosquito hosts. S. marcescens ano1 and ano2 possessed multiple mechanisms for antagonism against other microorganisms, including production of bacteriocins and multi-antibiotic resistance determinants. These genes contributing to potential anti-malaria activity including serralysins, hemolysins and chitinases are only found in some Serratia species. It is interesting that genome sequences in S. marcescens ano1 and ano2 are distinctly different from those in Serratia sp. Ag1 and Ag2 which were isolated from Anopheles gambiae. Compared to Serratia sp. Ag1 and Ag2, S. marcescens ano1 and ano2 have more rRNAs and many important genes involved in commensal and anti-parasite traits.

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Section: Discussionmentioning

confidence: 98%

Genomic, Physiologic, and Symbiotic Characterization of Serratia marcescens Strains Isolated from the Mosquito Anopheles stephensi

2017

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“…TEL-2014, which was found to be symbiotically associated with Serratia sp. TEL [13] , [14] . The infective juveniles of this entomopathogenic nematode were collected from freshly prepared White traps and surface sterilised with 0.1% sodium hypochlorite for 3 h in sterile 1.5 ml Eppendorf tubes.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Whole genome sequence of Oscheius sp. TEL-2014 entomopathogenic nematodes isolated from South Africa

et al. 2016

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We present the annotation of the draft genome sequence of Oscheius sp. TEL-2014 (Genbank accession number KM492926). This entomopathogenic nematode was isolated from grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Oscheius sp. Strain TEL has a genome size of 110,599,558 bp and a GC content of 42.24%. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession number LNBV00000000.

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Inspecting the pathogenicity of the integument of Oscheius basothovii following sterilization with sodium hypochlorite

Lephoto

Gray

2022

Biological Control

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Genome sequencing and annotation of Serratia sp. strain TEL

Cited by 3 publications

References 5 publications

Genomic, Physiologic, and Symbiotic Characterization of Serratia marcescens Strains Isolated from the Mosquito Anopheles stephensi

Genomic, Physiologic, and Symbiotic Characterization of Serratia marcescens Strains Isolated from the Mosquito Anopheles stephensi

Whole genome sequence of Oscheius sp. TEL-2014 entomopathogenic nematodes isolated from South Africa

Inspecting the pathogenicity of the integument of Oscheius basothovii following sterilization with sodium hypochlorite

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