Genome sequencing of the important oilseed crop Sesamum indicumL

Zhang, Haiyang; Miao, Hongmei; Wang, Lei; Qu, Lingbo; Liu, Hongyan; Wang, Qiang; Yue, Meiwang

doi:10.1186/gb-2013-14-1-401

Cited by 127 publications

(99 citation statements)

References 64 publications

(53 reference statements)

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“…The published sesame genome sequence (Zhang et al, 2013) was used to identify candidate genes that are known to function in other plants in the signal transduction pathways in response to abiotic as well as biotic stress, some of them possibly working as cross-talk factors. To check whether overexpression of SindOLP in sesame results in activation of phytohormone defense signaling, transcriptional profiles of the selected genes that function in the two pathways were studied.…”

Section: Resultsmentioning

confidence: 99%

“…These sequences were used to find corresponding genes in sesame from the sesame genome sequence (Zhang et al, 2013). The details of the work are given in Results Section.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, there is no study thus far about any gene regulation in sesame in response to any kind of stress. The recent availability of the sesame genome sequence (Zhang et al, 2013) is likely to change that scenario.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame

2017

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Osmotin-like proteins (OLPs), of PR-5 family, mediate defense against abiotic, and biotic stresses in plants. Overexpression in sesame of an OLP gene (SindOLP), enhanced tolerance against drought, salinity, oxidative stress, and the charcoal rot pathogen. SindOLP was expressed in all parts and localized to the cytosol. The transgenic plants recovered after prolonged drought and salinity stress, showing less electrolyte leakage, more water content, longer roots, and smaller stomatal aperture compared to control plants. There was an increase in osmolytes, ROS-scavenging enzymes, chlorophyll content, proline, secondary metabolites, and reduced lipid peroxidation in the transgenic sesame under multiple stresses. The OLP gene imparted increased tolerance through the increased expression of three genes coding for ROS scavenging enzymes and five defense-related marker genes functioning in the JA/ET and SA pathways, namely Si-Apetala2, Si-Ethylene-responsive factor, Si-Defensin, Si-Chitinase, and Si-Thaumatin-like protein were monitored. The transgenic lines showed greater survival under different stresses compared to control through the integrated activation of multiple components of the defense signaling cascade. This is the first report of transgenic sesame and first of any study done on defense-related genes in sesame. This is also the first attempt at understanding the molecular mechanism underlying multi-stress tolerance imparted by an OLP.

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Section: Resultsmentioning

confidence: 99%

“…These sequences were used to find corresponding genes in sesame from the sesame genome sequence (Zhang et al, 2013). The details of the work are given in Results Section.…”

Section: Methodsmentioning

confidence: 99%

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Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame

2017

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“…Further the data would be useful in identifying parents for heterosis breeding, conservation, and genetic improvement for yield and other desirable traits in sesame. The sesame genome sequence recently studied by Zhang et al (2013b) has provided and opened up new challenges for the present scientists to improve the crop efficiently at each and every level.…”

Section: Discussionmentioning

confidence: 99%

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

et al. 2017

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Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of 256 bands were obtained with RAPD primers, of which 191 were polymorphic. SSR primers gave 64 DNA bands, of which all of were polymorphic. The Jaccardʼs similarity coefficient of RAPD, SSR, and pooled RAPD and SSR data ranged from 0.510 to 0.885, 0.167 to 0.867, and 0.505 to 0.853, respectively. Maximum polymorphic information content was reported with SSRs (0.194) compared to RAPDs (0.186). Higher marker index was observed with RAPDs (1.426) than with SSRs (0.621). Similarly, maximum resolving power was found with RAPD (4.012) primers than with SSRs (0.884). The RAPD primer RPI-B11 and SSR primer S16 were the most informative in terms of describing genetic variability among the varieties under study. At a molecular level, the seed coat colour was distinguishable by the presence and absence of a group of marker amplicon/s. White and brown seeded varieties clustered close to each other, while black seeded varieties remained distanced from the cluster. In the present study, we found higher variability in Sesamum indicum L. using RAPD and SSR markers and these could assist in DNA finger printing, conservation of germplasm, and crop improvement.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0578-4) contains supplementary material, which is available to authorized users.

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“…Recent results indicated that no more than 8% lines among 2210 Chinese and foreign sesame germplasm resources presented the high resistance to Fos in artificial SFW nursery (data not published). The narrow genetic basis in sesame germplasm resources suggests that improving the sesame resistance level would be unlikely to be solved in the near future (Zhang et al , 2013. Till now, the FW resistance heritability and molecular mechanism analyses are not performed intensively in sesame.…”

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confidence: 99%

Transcriptome comparison of resistant and susceptible sesame (Sesamum indicum L.) varieties inoculated with Fusarium oxysporum f. sp. sesami

et al. 2016

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Sesame Fusarium wilt (FW) is a worldwide and hardly controlled fungus disease in sesame. Till now, the molecular resistance mechanism of sesame to FW remains unknown. The two resistant and susceptible sesame varieties, that is ‘Yuzhi 11’ and Rongxian black sesame (RXBS), were inoculated with Fusarium oxysporum f. sp. sesami (Fos) pathogens for 0, 6, 24 or 24 and 48h, respectively. Then, transcriptome samples were sequenced and surveyed systematically using RNA‐seq technology. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that ‘phenylpropanoid biosynthesis’ pathway may play more important role in sesame resistance after Fos inoculation. Furthermore, qRT‐PCR results demonstrated that the phenylpropanoid pathway with an emphasis on the synthesis of lignin contributes to the sesame disease resistance. Moreover, activity of phenylalanine ammonia lyase (PAL) and peroxidase (POD) proved the differences of gene expression patterns in the two sesame phenotypes. Overall, we speculated that the difference of FW symptoms between the resistant and susceptible plants might depend on whether the plants could activate the pathway timely and efficiently or not. The findings would help to clarify the molecular mechanism of sesame resistance to FW.

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Genome sequencing of the important oilseed crop Sesamum indicumL

Abstract: The Sesame Genome Working Group (SGWG) has been formed to sequence and assemble the sesame (Sesamum indicum L.) genome. The status of this project and our planned analyses are described.

Cited by 127 publications

References 64 publications

Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame

Overexpression of a New Osmotin-Like Protein Gene (SindOLP) Confers Tolerance against Biotic and Abiotic Stresses in Sesame

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

Transcriptome comparison of resistant and susceptible sesame (Sesamum indicum L.) varieties inoculated with Fusarium oxysporum f. sp. sesami

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