Genome shuffling: Progress and applications for phenotype improvement

Genome Shuffling of Clostridium acetobutylicum CICC 8012 for Improved Production of Acetone–Butanol–Ethanol (ABE)

“…This method had been widely applicable to the improvement of most production pathways in microorganisms [7,12,14,20]. Here we describe its application to improve the production of ABE in Clostridium acetobutylicum CICC 8012.…”

Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance

“…However, these approaches could be time consuming and labor intensive (Gong et al 2009). Metabolic engineering tools were also employed previously to enhance 1-butanol tolerance in microorganisms (Tomas et al 2003), but they usually require detailed genetic knowledge and information on genotype-phenotype relationships, which often constrains their application in strain engineering (Kern et al 2007).…”

Strategies of Strain Improvement of Industrial Microbes

“…Lipase production was increased by 317 % over the starting strain Penicillium expansum FS8486 by two rounds of genome shuffl ing (Lin et al 2007 ). Similarly, the production of epithiolone, the anticancer drug produced by the bacterium Sorangium cellulosum strain So157-2, was improved in fusants and increased about 130 times after two rounds of genome shuffl ing (Gong et al 2009 ). Thus, a lot of time is saved which was initially invested in classical mutagenesis by the process of genome shuffl ing.…”