Genome structure and organization of a member of a novel and distinct species of the genus Caulimovirus associated with dahlia mosaic

Abstract

The genome structure and organization of a new and distinct caulimovirus that is widespread in dahlia (Dahlia variabilis) was determined. The double-stranded DNA genome was ca. 7.0 kb in size and shared many of the features of the members of the genus Caulimovirus, such as the presence of genes potentially coding for the movement protein, the inclusion body protein, and the reverse transcriptase (RT), and an intergenic region consisting of a potential 35S promoter. However, the virus differed from the previously described dahlia mosaic caulimovirus and other known caulimoviruses in that the aphid transmission factor (ATF) was absent and the putative coat protein contained a C-terminal deletion and was fused in-frame with the RT. Sequence identity at the amino acid level with known caulimoviruses including a previously reported caulimovirus from dahlia was low and ranged from 32 to 72%. The absence of an ATF and the highly divergent nature of the genomic sequence are characteristics of this new caulimovirus that is widely associated with dahlia.

Incidence of Multiple and Distinct Species of Caulimoviruses in Dahlia (Dahlia variabilis)

“…Additionally, testing showed that DCMV was more prevalent than DMV, and mixed infections were common. This is supported by earlier studies (Pahalawatta et al, 2008a;Pappu et al, 2005). Infection of dahlia plants by DMV-D10 was often asymptomatic (greater than 50% of tested samples) but sometimes resulted in showing symptoms (mentioned previously).…”

“…In addition to DMV, which was characterized at the molecular level (Richins and Shepherd, 1983), recent studies have revealed the presence of at least two more caulimoviruses, tentatively referred to as DMV-D10 and Dahlia common mosaic virus (DCMV) (Nicolaisen, 2003;Pahalawatta et al, 2008a;Pappu et al, 2008). DMV-D10 shares limited sequence similarity with known caulimoviruses, including DMV, although the genome organization clearly suggests that it is a member of Caulimovirus (Pahalawatta et al, 2008a). DMV-D10 was found to be widespread based on the surveys conducted in the United States and Europe (Pahalawatta et al, 2007;Pappu et al, 2005).…”

Population of Endogenous Pararetrovirus Genomes in Carrizo Citrange

“…Many are silent, but some may replicate and cause viral infection under certain conditions (1). These endogenous pararetrovirus (EPRVs) have been reported to cause diseases, including banana streak virus (2), tobacco vein-clearing virus (3), dahlia mosaic virus (D10) (4), cassava vein mosaic virus (5), and petunia vein-clearing virus (PVCV) (6). EPRVs can be induced to replicate by hybridization, tissue culture, heat, drought stress, and wounding (7).…”

Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

“…, 2007; Pappu et al. , 2008), these two new caulimoviruses showed significant sequence divergence compared to that of DMV (Pahalawatta et al. , 2008b; Pappu et al.…”

“…In addition to DMV, it was recently shown that there are at least two more distinct caulimoviruses associated with dahlia. Tentatively referred to as Dahlia common mosaic virus (DCMV) and DMV-D10 (Pahalawatta et al, 2007;Pappu et al, 2008), these two new caulimoviruses showed significant sequence divergence compared to that of DMV (Pahalawatta et al, 2008b;Pappu et al, 2008). Both DCMV and DMV-D10 were found to be widespread based on surveys conducted in the USA and Europe (Pappu et al, 2005;Pahalawatta et al, 2007;Eid et al, 2009).…”