Genome variability in the altered variants of Vibrio cholerae biovar El Tor isolated in Russia

Smirnova, N. I.; Zadnova, S. P.; Shashkova, Anna; Кутырев, Владимир

doi:10.3103/s0891416811030062

Cited by 14 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The other 30 strains, including one from seawater, have been imported into Russia and Ukraine from different countries endemic for cholera over the last two decades (1991-2012) and belonged to the above mentioned genovariants containing a DNA fragment of the classical cholera vibrio biovar in their genome. The totality of our data show that the repre sented strains of the genovariants differed from one another in the structure of their genome [19,21]. The ctxB1 allele was present in the genome of 90% of the studied genovariants.…”

Section: Resultsmentioning

confidence: 78%

“…Furthermore, the genovariants isolated over the last two decades (1991-2001 and 2002-2012) differed from one another in the structure of pandemicity island VSP II. Whereas the genovariants isolated in 1991-2001 carried an intact VSP II or VSP II with a short deletion embracing four genes (VSP IIΔvc0495 vc0498), all the genovariants imported to Russia over the last decade (2002-2012) had VSP II with an extended deletion, or VSP IIΔvc0495 vc0512 (see Table 2) [19,21]. The cause for this variability of VSP II in the genovariants is still unclear.…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

MLVA typing of clinical Vibrio cholerae strains isolated during different periods of the current cholera pandemic

Smirnova

Кульшань

Krasnov

2015

Mol. Genet. Microbiol. Virol.

Self Cite

View full text Add to dashboard Cite

MLVA typing was performed for five variable loci of the 52 Vibrio cholerae El Tor biovar strains iso lated before the onset and in different periods of the seventh cholera pandemic, as well as for eight strains of the classical V. cholerae biovar-the causative agent of the preceding Asiatic cholera pandemics. It has been shown that the studied strains, which differ in their molecular genetic properties and are characterized by 38 MLVA types, form seven clonal clusters. Clusters I and II were formed, respectively, by V. cholerae strains of the classical biovar and prepandemic V. cholerae strains, whereas clusters III-VII were formed by V. cholerae El Tor biovar strains isolated in different periods of the latest pandemic. It has been shown that the MLVA typing allows us to clearly differentiate V. cholerae El Tor biovar strains into typical and genetically altered strains. The genetically altered strains are characterized by higher virulence compared with the typical strains, as well as the presence of the ctxB gene of the classical cholera vibrio in the genome. The study showed the possibility of differentiating genovariants isolated in different periods and having different structures of pandemicity island VSP II. Since the VSP II structure directly correlates with the epidemic potential of the strains, the possibility of differentiating isolates with intact and deleted VSP II using the MLVA method deserves further research. Based on the obtained results, we can propose a polyclonal origin of different strains of genovariants.

show abstract

Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 95%

MLVA typing of clinical Vibrio cholerae strains isolated during different periods of the current cholera pandemic

Smirnova

Кульшань

Krasnov

2015

Mol. Genet. Microbiol. Virol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…), азии, африке, европе, сШа, океании, австралии (1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988)(1989)(1990) гг.) [4][5][6][7][8][9][10][11].…”

unclassified

Test-System for Identification of Typical and Genetically Altered Variants of Cholera Vibrios, Biovar El Tor, Using Real-Time PCR

Савельев

Куличенко

Savel’Eva

et al. 2022

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

The aim of the work was to analyze and assess the results of using PCR test-system “V. cholerae variant ctxB-rtxC FL genes” for identification of Vibrio cholerae O1 with differentiation between typical toxigenic and genetically modified variants in a multiplex format with a real-time hybridization-fluorescent recording of results. Materials and methods. To achieve this goal, a set of reagents for the PCR test-system “V. cholerae O1 variant ctxB-rtxC FL genes”, as well as a method for identifying V. cholerae O1 with differentiation between typical toxigenic and genetically altered variants were utilized. The specificity, specific activity and sensitivity of the developed test-system by the example of 35 V. cholerae O1 strains, 6 V. cholerae non-O1 strains, 5 heterologous strains (Shigella zonnei – 2 strains, one Salmonella typhimurium strain, S. enteritidis, Escherichia coli) were investigated. Results and discussion. The panel of reagents for the PCR test system “V. cholerae variant ctxB-rtxC FL genes” detects DNA fragments of the ctxBCl, ctxBEl, rtxC genes in the genome of toxigenic V. cholerae О1 (has specific activity, analytical sensitivity 1·103 GE/ml) and does not detect these genes in non-toxigenic V. cholerae O1 and non-O1, as well as in heterologous strains of microorganisms (100 % specificity). RF Patent No. 2732448 was granted for the PCR test-system “V. cholerae variant ctxB-rtxC FL genes”. It can be used to increase the efficiency of the epidemiological surveillance system and to carry out a justified scope of anti-epidemic measures in the event of cholera importation into the territory of the Russian Federation.

show abstract

“…На протяжении седьмой пандемии холеры отмечено формирование новых клонов с различными, часто уникальными сочетаниями генов, связанных с проявлением вирулентности. При этом наряду с появлением возбудителей холеры, обладающих более мощным эпидемическим потенциалом [9,19], наблюдается возникновение высоковирулентных штаммов, лишенных генов холерного токсина (СТ), но экспрессирующих гены дополнительных факторов патогенности. Такие штаммы встречаются среди представителей как О1, так и неО1/неО139 серогрупп [5,20,28,41].…”

unclassified

Cholera Vibrio Virulence Strategy and Ways of its Realization (Scientific Review)

Монахова¹

2013

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

The basic ways of Vibrio cholerae virulence strategy realization through acquisition and expression of genes of various toxic substances are discussed. Considered are molecular mechanisms responsible for interaction between host organism and cholera vibrios, as well as for genetic information exchange, for accumulation and loss of determinants of factors, which are non-identical structurally but functionally similar. Based on the analysis of literature data and personal observations put forward is a conception of pathogenicity factor intersubstitutability allowing for restoration and maintenance of pathogenetic potential of severe diarrheal disease agents among the strains deprived of cholera toxin genes.

show abstract

Genome variability in the altered variants of Vibrio cholerae biovar El Tor isolated in Russia

Cited by 14 publications

References 25 publications

MLVA typing of clinical Vibrio cholerae strains isolated during different periods of the current cholera pandemic

MLVA typing of clinical Vibrio cholerae strains isolated during different periods of the current cholera pandemic

Test-System for Identification of Typical and Genetically Altered Variants of Cholera Vibrios, Biovar El Tor, Using Real-Time PCR

Cholera Vibrio Virulence Strategy and Ways of its Realization (Scientific Review)

Contact Info

Product

Resources

About