Genome-wide analysis of differentially expressed genes and the modulation of PEDV infection in Vero E6 cells

Abstract

PEDV remains one of the most important swine diseases that infects pigs of all ages. It causes devastating viral enteric disease in piglets with a high mortality rate, leading to significant threats and huge economic loss to the pork industry. In this study, a transcriptomic shotgun sequencing (RNA-Seq) procedure was used to study gene responses against PEDV infection. Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR signaling pathway activator-MHY1485, and inhibitor-PP242 were used to study the antiviral function. Results revealed that the IRF3 was significantly up-regulated post-PEDV infection. Although most of the IFN-regulatory and -related genes evaluated in this study were either down-regulated or remained unchanged, IL11 behaved significantly up-regulated, with the peak at 16 hpi. Nearly 90% of PEDV infections were suppressed in the PP242 pretreated cells whereas the reverse effect was observed in the MYH1485 pretreated cells. Results indicated that the mTOR signaling pathway played a vital role in the PEDV antiviral regulation in the Vero E6 cells. Future studies will contribute to better understand the cellular antiviral mechanism against PEDV.

Porcine endemic diarrhea virus infection regulates long noncoding RNA expression

“…The JAK/STAT pathways were identified from both analyses of the genes in the vicinity of lncRNAs and mRNAs, indicating their vital roles during PEDV infection. Antigen processing and presentation, cytokine-cytokine receptor interaction, and metabolic pathways were also identified in another study (Zhang et al, 2018), suggesting these molecules may also be important to host responses against PEDV infection.…”

Transcriptome analysis reveals modulation of the STAT family in PEDV-infected IPEC-J2 cells

“…Previous studies provided transcriptomic and proteomic data for PEDV-infected Vero cells that offered the first valuable insight to better understand the host response to PEDV infection [ 16 , 44 , 45 ]. However, these reports contained some contradictory results.…”

Isolation and identification of a variant subtype G 2b porcine epidemic diarrhea virus and S gene sequence characteristic

“…Moreover, complicated culture conditions are also the important reason for curbing the prevention and treatment of PEDV. We referenced to the method that isolating virus with trypsin as previous reports (Hofmann and Wyler, 1988;Kusanagi et al, 1992;Lee et al, 2015;Li et al, 2015;Zhang et al, 2018). Then, a strain of PEDV, CH/JLDH/2016, was isolated and identified in our study.…”