PEDV remains one of the most important swine diseases that infects pigs of all ages. It causes devastating viral enteric disease in piglets with a high mortality rate, leading to significant threats and huge economic loss to the pork industry. In this study, a transcriptomic shotgun sequencing (RNA-Seq) procedure was used to study gene responses against PEDV infection. Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR signaling pathway activator-MHY1485, and inhibitor-PP242 were used to study the antiviral function. Results revealed that the IRF3 was significantly up-regulated post-PEDV infection. Although most of the IFN-regulatory and -related genes evaluated in this study were either down-regulated or remained unchanged, IL11 behaved significantly up-regulated, with the peak at 16 hpi. Nearly 90% of PEDV infections were suppressed in the PP242 pretreated cells whereas the reverse effect was observed in the MYH1485 pretreated cells. Results indicated that the mTOR signaling pathway played a vital role in the PEDV antiviral regulation in the Vero E6 cells. Future studies will contribute to better understand the cellular antiviral mechanism against PEDV.

Swine influenza virus (SIV) is an important pathogen. Vaccination is the most effective way to prevent the disease. The objective of this study was to evaluate the protective efficacy of a commercial inactivated bivalent swine influenza vaccine (H1N1/H3N2). Vaccinated pigs with the three batches (# 201604, #201605, and #201606) had no clinical signs of disease, no viral shedding, and any lung lesions after the wild-type SIV-H1N1 LN and SIV-H3N2 HLJ challenge, and the unvaccinated pigs showed clinical signs of disease, had viral shedding, and severe lung lesions. Our results clearly demonstrate that three batches of vaccine products provided complete protection against the wild-type SIV-H1N1 LN and SIV-H3N2 HLJ challenge.