Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

Abstract

Introduction: Cotton is an important economic crop to provide natural fibers as raw materials to textile industry, and is significantly affected by biotic and abiotic stress during the whole growth stage, in which Verticillium wilt (VW) caused by Verticillium dahliae is one of the most destructive disease to lead to a significant yield reduction. Heat shock proteins (Hsps) are important molecular chaperones, and play crucial roles in plant growth, development, resistance to biotic and abiotic stress. Hsp40 and Hsp70 are two key Hsps in cell chaperone network, however, the function and regulatory mechanism of Hsp40 and Hsp70 members in VW resistance and abiotic stress in cotton are largely unknown.Methods and Results: Herein, a systematic and comprehensive analysis of Hsp40s and Hsp70s in four cotton species of Gossypium arboretum, G. raimondii, G. hirsutum, and G. barbadense were performed. A total of 291 Hsp40s and 171 Hsp70s identified in four Gossypium species. Sequence analysis revealed that all Hsp40 proteins contained J domain that provides the binding sites to Hsp70. Protein-protein interaction prediction analysis displayed that GhHsp40-55 might interact with GhHsp70-2 and GhHsp70-13, suggesting their potential function as protein complex. Promoter cis-acting element analysis demonstrated that multiple cis-elements related to disease and stress response consists in GhHsp40 and GhHsp70 promoters. Further expression analysis showed that eight GhHsp40s (Hsp40-2,4,8,11,20,23,53,55) and seven GhHsp70s (Hsp70-2,3,6,8,13,19,22) were up-regulated after V. dahliae infection. In addition, five GhHsp40s (Hsp40-2,8,11,53,55) and four GhHsp70s (Hsp70-3,6,8,13) were up-regulated after salt treatment, six GhHsp40s (Hsp40-4,11,20,23) and three GhHsp70s (Hsp70-2,8,19) were up-regulated after drought treatment, four GhHsp40s (Hsp40-2,11,20,23) and four GhHsp70s (Hsp70-3,6,19,22) were up-regulated after temperature treatment, suggesting these Hsps have possible important function in the process of abiotic stress response.Discussion: Our results lay a foundation for understanding the function of Hsp40 and Hsp70 in the resistance against V. dahliae and abiotic stress, and elucidating the regulatory mechanism of the protein complex, evolution and molecular mechanism under stress.

Unveiling Cotton's Defense: Harnessing GthZIM17-1 Inhibition for Verticillium Wilt Resistance

“…However, in plants, it does not function as a transcription factor but instead serves as a dedicated chaperone protein for Hsp70, facilitating the mitochondrial protein importation process. Studies have shown that members of the cotton heat shock protein family, Hsp70s, can be induced by both biotic and abiotic stresses such as salt, drought, and Verticillium wilt [44,45]. The evolution of polyploid species often involves the loss of a large number of genes or the duplication of gene copies, leading to increased genetic diversity and plasticity in polyploid species [46].…”

Research Status and Prospects on the Construction Methods of Temperature and Humidity Environmental Models in Arbor Tree Cuttage

“…The substrate is composed of vermiculite. At the time of cutting, the relative humidity of the air and the moisture of the substrate are required to be 80-90% and 50-60%, respectively [95], which can form a film of water on the cutting surface [96]. Higher air humidity can reduce the evaporation of substrate water so that the substrate can maintain the appropriate humidity to reduce the water loss from the cutting cells to avoid the cuttings' dehydration and an injury to seedlings [97].…”