Genome-Wide Analysis of Light- and Temperature-Entrained Circadian Transcripts in Caenorhabditis elegans

Linden, Alexander M. van der; Beverly, Matthew; Kadener, Sebastián; Rodriguez, Joseph; Wasserman, Sara; Rosbash, Michael; Sengupta, Piali

doi:10.1371/journal.pbio.1000503

Cited by 66 publications

(112 citation statements)

References 87 publications

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“…The lack of rhythmicity of the clock gene ortholog, lin-42, shown here as well as by others (11,26), is thus puzzling. However, even in metazoans that are more closely related, such as Drosophila and mice, orthologous "clock genes" can be regulated distinctly, with the proteins using alternative heterodimerization partners, for instance (51).…”

mentioning

confidence: 55%

“…S1). We then investigated expression of transcripts that had been reported as rhythmic in protocols that used higher amplitude temperature cycles (11). Although the expression of many of these genes was dynamic (decreasing or increasing on release to constant conditions), only one of nine (B0507.8) (11) was significantly rhythmic (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Over the last decade, there have been numerous reports of daily rhythms in the soil-dwelling nematode, C. elegans (6)(7)(8)(9)(10)(11)(12), although the connection between molecules and behavior has been lacking. Reasons for this may include a lack of known clock gene homologs as starting points, a number of low-amplitude behavioral rhythms, and the use of conditions that are far from ecological.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, protocols used for clock research on the nematode, C. elegans (6)(7)(8)(9)(10)(11)(12), have shown circadian rhythms in behaviors (e.g., locomotor activity, defecation, and pharyngeal pumping rate), metabolism (e.g., resistance to osmotic stress), or the expression of hundreds of genes without a connection to rhythmic behaviors. Clearly, taken together, one would conclude that the nematode has a circadian system.…”

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confidence: 99%

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Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Olmedo

O’Neill

Edgar

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Circadian clocks provide a temporal structure to processes from gene expression to behavior in organisms from all phyla. Most clocks are synchronized to the environment by alternations of light and dark. However, many organisms experience only muted daily environmental cycles due to their lightless spatial niches (e.g., caves or soil). This has led to speculation that they may dispense with the daily clock. However, recent reports contradict this notion, showing various behavioral and molecular rhythms in Caenorhabditis elegans and in blind cave fish. Based on the ecology of nematodes, we applied low-amplitude temperature cycles to synchronize populations of animals through development. This entrainment regime reveals rhythms on multiple levels: in olfactory cued behavior, in RNA and protein abundance, and in the oxidation state of a broadly conserved peroxiredoxin protein. Our work links the nematode clock with that of other clock model systems; it also emphasizes the importance of daily rhythms in sensory functions that are likely to impact on organism fitness and population structure.

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confidence: 55%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Olmedo

O’Neill

Edgar

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…However, the study of circadian rhythms in this model is far from complete, and the mechanism underlying the molecular basis of the central pacemaker remains elusive. Numerous circadian cycles have been described in the nematode at different levels, including behavior (locomotor activity) (10)(11)(12), physiology (defecation rate, pharyngeal pumping rate, and olfaction) (13,14), metabolism (abiotic and biotic stress tolerance, food and oxygen consumption) (15,16), protein activity and regulation (14,17,18), and gene expression (19). Recent exhaustive bioinformatics searches have identified proteins of relatively high homology to clock components from other species (20).…”

mentioning

confidence: 99%

Circadian rhythms identified in Caenorhabditis elegans by in vivo long-term monitoring of a bioluminescent reporter

Goya

Romanowski

Caldart

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Circadian rhythms are based on endogenous clocks that allow organisms to adjust their physiology and behavior by entrainment to the solar day and, in turn, to select the optimal times for most biological variables. Diverse model systems-including mice, flies, fungi, plants, and bacteria-have provided important insights into the mechanisms of circadian rhythmicity. However, the general principles that govern the circadian clock of Caenorhabditis elegans have remained largely elusive. Here we report robust molecular circadian rhythms in C. elegans recorded with a bioluminescence assay in vivo and demonstrate the main features of the circadian system of the nematode. By constructing a luciferase-based reporter coupled to the promoter of the suppressor of activated let-60 Ras (sur-5) gene, we show in both population and single-nematode assays that C. elegans expresses ∼24-h rhythms that can be entrained by light/dark and temperature cycles. We provide evidence that these rhythms are temperature-compensated and can be re-entrained after phase changes of the synchronizing agents.In addition, we demonstrate that light and temperature sensing requires the photoreceptors LITE and GUR-3, and the cyclic nucleotidegated channel subunit TAX-2. Our results shed light on C. elegans circadian biology and demonstrate evolutionarily conserved features in the circadian system of the nematode.C. elegans | circadian rhythms | luminescence | temperature | light

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Explaining general anesthesia: A two‐step hypothesis linking sleep circuits and the synaptic release machinery

Swinderen

Kottler

2014

BioEssays

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Several general anesthetics produce their sedative effect by activating endogenous sleep pathways. We propose that general anesthesia is a two-step process targeting sleep circuits at low doses, and synaptic release mechanisms across the entire brain at the higher doses required for surgery. Our hypothesis synthesizes data from a variety of model systems, some which require sleep (e.g. rodents and adult flies) and others that probably do not sleep (e.g. adult nematodes and cultured cell lines). Non-sleeping systems can be made insensitive (or hypersensitive) to some anesthetics by modifying a single pre-synaptic protein, syntaxin1A. This suggests that the synaptic release machinery, centered on the highly conserved SNARE complex, is an important target of general anesthetics in all animals. A careful consideration of SNARE architecture uncovers a potential mechanism for general anesthesia, which may be the primary target in animals that do not sleep, but a secondary target in animals that sleep.

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Genome-Wide Analysis of Light- and Temperature-Entrained Circadian Transcripts in Caenorhabditis elegans

Abstract: Transcriptional profiling experiments identify light- and temperature-entrained circadian transcripts in C. elegans.

Cited by 66 publications

References 87 publications

Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Circadian regulation of olfaction and an evolutionarily conserved, nontranscriptional marker in Caenorhabditis elegans

Circadian rhythms identified in Caenorhabditis elegans by in vivo long-term monitoring of a bioluminescent reporter

Explaining general anesthesia: A two‐step hypothesis linking sleep circuits and the synaptic release machinery

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