Genome-wide analysis of sulfotransferase genes and their responses to abiotic stresses in Chinese cabbage (Brassica rapa L.)

Jin, Lü; Ouyang, Ning; Huang, Yong; Liu, Chunlin; Ruan, Ying

doi:10.1371/journal.pone.0221422

Cited by 14 publications

(11 citation statements)

References 41 publications

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“…Seven sulfotransferases ( SOT ), enzymes catalyzing the sulfation process, were also increased after salt stress both in Adina and Zhaodong. The changes of the SOT s in our study are consistent with what is documented in transcriptional assays, such as the expression of AtSOT12 ( Hirschmann et al., 2014 ), BrSOT s ( Jin et al., 2019 ) and SjSOT s ( Lu et al., 2020 ) after exposure to salt stress. A leading hypothesis is that the sulfation of polysaccharides that causes the modification of the composition of cell walls is the main reason for increased salt tolerance in plants ( Hirschmann et al., 2014 ).…”

Section: Discussionsupporting

confidence: 90%

Comparative time-course transcriptome analysis of two contrasting alfalfa (Medicago sativa L.) genotypes reveals tolerance mechanisms to salt stress

Cai²,

Ma³

et al. 2022

Front. Plant Sci.

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Salt stress is a major abiotic stress affecting plant growth and crop yield. For the successful cultivation of alfalfa (Medicago sativa L.), a key legume forage, in saline-affected areas, it’s essential to explore genetic modifications to improve salt-tolerance.Transcriptome assay of two comparative alfalfa genotypes, Adina and Zhaodong, following a 4 h and 8 h’s 300 mM NaCl treatment was conducted in this study in order to investigate the molecular mechanism in alfalfa under salt stress conditions. Results showed that we obtained 875,023,571 transcripts and 662,765,594 unigenes were abtained from the sequenced libraries, and 520,091 assembled unigenes were annotated in at least one database. Among them, we identified 1,636 differentially expression genes (DEGs) in Adina, of which 1,426 were up-regulated and 210 down-regulated, and 1,295 DEGs in Zhaodong, of which 565 were up-regulated and 730 down-regulated. GO annotations and KEGG pathway enrichments of the DEGs based on RNA-seq data indicated that DEGs were involved in (1) ion and membrane homeostasis, including ABC transporter, CLC, NCX, and NHX; (2) Ca2+ sensing and transduction, including BK channel, EF-hand domain, and calmodulin binding protein; (3) phytohormone signaling and regulation, including TPR, FBP, LRR, and PP2C; (4) transcription factors, including zinc finger proteins, YABBY, and SBP-box; (5) antioxidation process, including GST, PYROX, and ALDH; (6) post-translational modification, including UCH, ubiquitin family, GT, MT and SOT. The functional roles of DEGs could explain the variations in salt tolerance performance observed between the two alfalfa genotypes Adina and Zhaodong. Our study widens the understanding of the sophisticated molecular response and tolerance mechanism to salt stress, providing novel insights on candidate genes and pathways for genetic modification involved in salt stress adaptation in alfalfa.

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Section: Discussionsupporting

confidence: 90%

Comparative time-course transcriptome analysis of two contrasting alfalfa (Medicago sativa L.) genotypes reveals tolerance mechanisms to salt stress

Cai²,

Ma³

et al. 2022

Front. Plant Sci.

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“…Other enzymes associated with secondary metabolism were FLAVONOL SULFOTRANSFERASE-LIKE, PHENYLALANINE AMMONIA-LYASE, ANTHOCYANIDIN 3-O-GLUCOSYLTRANSFERASE 5-LIKE, and ANTHOCYANIDIN SYNTHASE. These enzymes are involved in defense against abiotic and biotic stresses (Landi et al ., 2015; Zhang & Liu, 2015; Qiu et al ., 2016; Jin et al ., 2019).…”

Section: Resultsmentioning

confidence: 99%

Plant age-dependent dynamics of annatto pigment (bixin) biosynthesis inBixa orellanaL.

Machado

Faria

Duarte

et al. 2022

Preprint

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Age affects the production of secondary metabolites, but how developmental cues regulate secondary metabolism remains poorly understood. Annatto (Bixa orellana L.) is a source of bixin, an apocarotenoid used in the world's food industry worldwide. Understanding how age-dependent mechanisms control bixin biosynthesis is of great interest for plant biology and for pharmaceutical, cosmetic, and textile industries. Here, we used genetic and molecular tools to unravel the role of the annatto age regulated miRNA156 (miR156) targeted SQUAMOSA PROMOTER BINDING PROTEIN LIKE (BoSPL) genes in secondary metabolism. Low expression of several BoSPL genes in miR156 overexpressing annatto plants (OE::156) impacted leaf ontogeny, reducing bixin production and increasing abscisic acid (ABA) levels. Modulation of BoCCD4;4 and BoCCD1 expression, key genes in lycopene cleavage, was associated with diverting the carbon flux from bixin to ABA, whereas upregulation of lycopene β cyclase genes implies the xanthophyll biosynthetic pathway acted as a carbon sink in OE::156 plants. Proteomic analyses revealed low accumulation of most secondary metabolite-related enzymes in OE::156 plants, suggesting that miR156 targeted BoSPLs are required to activate several annatto secondary metabolic pathways. Our findings suggest that carbon flux in B. orellana OE::156 leaves was redirected from bixin to ABA production, indicating an age-dependent leaf dynamics of bixin biosynthesis. Importantly, our study opened a new venue to future annatto breeding programs aiming to improve bixin output.

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“…( Leitão et al., 2021 ) indicated that accumulation of sulfurtransferase was increased under stress induced by pharmaceutical contamination. Also, several studied illustrated that sulfurtransferases play a main role in plant response to abiotic stress such as osmotic, salt and hormone stress ( Jin et al., 2019 ). Overall, increased expression of cytosolic sulfotransferase 4 and thiosulfate/3-mercaptopyruvate sulfurtransferase 1 indicated a wide range of changes associated with osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

Proteomics approach to investigating osmotic stress effects on pistachio

et al. 2023

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Osmotic stress can occur due to some stresses such as salinity and drought, threatening plant survival. To investigate the mechanism governing the pistachio response to this stress, the biochemical alterations and protein profile of PEG-treated plants was monitored. Also, we selected two differentially abundant proteins to validate via Real-Time PCR. Biochemical results displayed that in treated plants, proline and phenolic content was elevated, photosynthetic pigments except carotenoid decreased and MDA concentration were not altered. Our findings identified a number of proteins using 2DE-MS, involved in mitigating osmotic stress in pistachio. A total of 180 protein spots were identified, of which 25 spots were altered in response to osmotic stress. Four spots that had photosynthetic activities were down-regulated, and the remaining spots were up-regulated. The biological functional analysis of protein spots exhibited that most of them are associated with the photosynthesis and metabolism (36%) followed by stress response (24%). Results of Real-Time PCR indicated that two of the representative genes illustrated a positive correlation among transcript level and protein expression and had a similar trend in regulation of gene and protein. Osmotic stress set changes in the proteins associated with photosynthesis and stress tolerance, proteins associated with the cell wall, changes in the expression of proteins involved in DNA and RNA processing occur. Findings of this research will introduce possible proteins and pathways that contribute to osmotic stress and can be considered for improving osmotic tolerance in pistachio.

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Genome-wide analysis of sulfotransferase genes and their responses to abiotic stresses in Chinese cabbage (Brassica rapa L.)

Cited by 14 publications

References 41 publications

Comparative time-course transcriptome analysis of two contrasting alfalfa (Medicago sativa L.) genotypes reveals tolerance mechanisms to salt stress

Comparative time-course transcriptome analysis of two contrasting alfalfa (Medicago sativa L.) genotypes reveals tolerance mechanisms to salt stress

Plant age-dependent dynamics of annatto pigment (bixin) biosynthesis inBixa orellanaL.

Proteomics approach to investigating osmotic stress effects on pistachio

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