Genome-Wide Analysis of the Cyclin Gene Family and Their Expression Profile in Medicago truncatula

Abstract

Cyclins, together with highly conserved cyclin-dependent kinases (CDKs), play an important role in the process of cell cycle in plants, but less is known about the functions of cyclins in legume plants, especially Medicago truncatula. Our genome-wide analysis identified 58, 103, and 51 cyclin members in the M. truncatula, Glycine max, and Phaseolus vulgaris genomes. Phylogenetic analysis suggested that these cyclins could be classified into 10 types, and the CycB-like types (CycBL1-BL8) were the specific subgroups in M. truncatula, which was one reason for the expansion of the B-type in M. truncatula. All putative cyclin genes were mapped onto their own chromosomes of each genome, and 9 segmental duplication gene pairs involving 20 genes were identified in M. truncatula cyclins. Determined by quantitative real-time PCR, the expression profiling suggested that 57 cyclins in M. truncatula were differentially expressed in 9 different tissues, while a few genes were expressed in some specific tissues. Using the publicly available RNAseq data, the expression of Mtcyclins in the wild-type strain A17 and three nodule mutants during rhizobial infection showed that 23 cyclins were highly upregulated in the nodulation (Nod) factor-hypersensitive mutant sickle (skl) mutant after 12 h of rhizobium inoculation. Among these cyclins, six cyclin genes were also specifically expressed in roots and nodules, which might play specific roles in the various phases of Nod factor-mediated cell cycle activation and nodule development. Our results provide information about the cyclin gene family in legume plants, serving as a guide for further functional research on plant cyclins.

A dual legume‐rhizobium transcriptome of symbiotic nodule senescence reveals coordinated plant and bacterial responses

“…Thirteen downregulated genes encode cyclins that were recently proposed to act during nodule initiation and development (Meng et al, 2020). Four downregulated genes are related to root/nodule development ( MtLBD16, MtPLT1, MtPLT3, MtPLT4 ), as well as the MtbHLH1 gene encoding a TF previously associated to nodule vascular patterning and nutrient exchanges (Godiard et al, 2011).…”

Exogenous 6-Benzyladenine Improves Waterlogging Tolerance in Maize Seedlings by Mitigating Oxidative Stress and Upregulating the Ascorbate-Glutathione Cycle

“…Examples include heavy metal stress (Khan et al, 2019), salt stress (Alam et al, 2019), drought stress (Wang H. H. et al, 2016), and heat stress (Li Z., et al, 2016). Cell division is the most fundamental process of any system of biotic growth and development and core-to-tissue growth in all organisms (Alim et al, 2012;Meng et al, 2020). Cytokinins are central regulators of plant growth and development, playing important regulatory functions in physiological processes, including cell division, apical dominance, plant growth, photomorphogenesis, nutrient translocation, and leaf senescence (Paul et al, 2016;Jan et al, 2019).…”

Overexpression Populus d-Type Cyclin Gene PsnCYCD1;1 Influences Cell Division and Produces Curved Leaf in Arabidopsis thaliana

“…Since three Arabidopsis thaliana CYCDs were first identified by complementation of a yeast G1 phase defective mutant strain [6], more CYCDs have been identified from various species [7][8][9]. However, most of them were characterized from herbaceous species like Arabidopsis, rice, and tobacco [10][11][12] Thus far, a total of ten AtCYCDs have been identified and divided into seven subfamilies (CYCD1-CYCD7) in A. thaliana [13].…”