Genome-wide analysis R2R3-MYB transcription factors in Xanthoceras sorbifolium Bunge and functional analysis of XsMYB30 in drought and salt stresses tolerance

Li, Jingbin; Zhou, Hui; Xiong, Chaowei; Peng, Zijia; Du, Wei; He, Lei; Wang, Li; Ruan, Chengjiang

doi:10.1016/j.indcrop.2022.114597

Cited by 23 publications

(13 citation statements)

References 55 publications

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“…The instability index (Ii) reflects the stability of protein, and protein with Ii value less than 40 is considered stable. Consistent with previous reports, most of IaMYBs (171/183) were considered unstable ( Li et al, 2022 ). The grand average of hydropathicity (GRAVY) reflects the hydrophobic or hydrophilic character of proteins.…”

Section: Discussionsupporting

confidence: 91%

Genome-wide identification of myeloblastosis gene family and its response to cadmium stress in Ipomoea aquatica

et al. 2022

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The myeloblastosis (MYB) proteins perform key functions in mediating cadmium (Cd) tolerance of plants. Ipomoea aquatica has strong adaptability to Cd Stress, while the roles of the I. aquatica MYB gene family with respect to Cd stress are still unclear. Here, we identified a total of 183 MYB genes in the I. aquatica genome (laMYB), which were classified into 66 1R-type IaMYB, 112 2R-type IaMYB, four 3R-type IaMYB, and one 4R-type IaMYB based on the number of the MYB repeat in each gene. The analysis of phylogenetic tree indicated that most of IaMYB genes are associated with the diverse biological processes including defense, development and metabolism. Analysis of sequence features showed that the IaMYB genes within identical subfamily have the similar patterns of the motif distributions and gene structures. Analysis of gene duplication events revealed that the dispersed duplication (DSD) and whole-genome duplication (WGD) modes play vital roles in the expansion of the IaMYB gene family. Expression profiling manifests that approximately 20% of IaMYB genes had significant role in the roots of I. aquatica under Cd stress. Promoter profiling implied that the differentially expressed genes might be induced by environmental factors or inherent hormones and thereby execute their function in Cd response. Remarkably, the 2R-type IaMYB157 with abundant light-responsive element G-box and ABA-responsive element ABRE in its promoter region exhibited very strong response to Cd stress. Taken together, our findings provide an important candidate IaMYB gene for further deciphering the molecular regulatory mechanism in plant with respect to Cd stress.

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Section: Discussionsupporting

confidence: 91%

Genome-wide identification of myeloblastosis gene family and its response to cadmium stress in Ipomoea aquatica

et al. 2022

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“…Using Actin as internal reference gene, several important downstream stress related genes of MYB were detected by qPCR. These genes were AtDREB1A ( Lu et al., 2017 ), AtCOR15a ( Steponkus et al., 1998 ), AtERD10B ( Du and Li, 2019 ), AtCOR47 ( Song et al., 2021 ), AtSnRK2.4 ( Nakashima and Yamaguchi-Shinozaki, 2013 ), AtRD29A ( Wu et al., 2022 ), AtSOD1 ( Li J. et al., 2022 ) and AtP5CS1 ( Chen et al., 2015 ). The reaction procedure and system of qPCR were the same as above methods.…”

Section: Methodsmentioning

confidence: 99%

MbMYBC1, a M. baccata MYB transcription factor, contribute to cold and drought stress tolerance in transgenic Arabidopsis

Liu¹,

Wang²,

Wang³

et al. 2023

Front. Plant Sci.

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Cold and drought stress considerably suppress the development of plants. In this study, a new MYB (v-myb avian myeloblastosis viral)TF gene, MbMYBC1, was isolated from the M. baccata and located in nucleus. MbMYBC1 has a positive response to low temperature and drought stress. After being introduced into Arabidopsis thaliana, the physiological indicators of transgenic Arabidopsis had corresponding changes under these two stresses, the activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) increased, electrolyte leakage rate (EL) and the content of proline increased, but the content of chlorophyll decreased. In addition, its overexpression can also activate the downstream expression of AtDREB1A, AtCOR15a, AtERD10B and AtCOR47 related to cold stress and AtSnRK2.4, AtRD29A, AtSOD1and AtP5CS1 related to drought stress. Based on these results, we speculate that MbMYBC1 can respond to cold and hydropenia signals, and can be used in transgenic technology to improve plant tolerance to low temperature and drought stress.

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“…Furthermore, signal peptide was predicted using SignalP ( http://www.cbs.dtu.dk/services/SignalP/ ) ( Duvaud et al., 2021 ). Subcellular localization was predicted using Plant-mPLoc ( http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/ ) and experiments were performed according to our previous research ( Li et al., 2022 ). Alignment was performed by using the program MUSCLE.…”

Section: Methodsmentioning

confidence: 99%

Identification of Camellia oleifera WRKY transcription factor genes and functional characterization of CoWRKY78

Xiong²,

Ruan³

et al. 2023

Front. Plant Sci.

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Camellia oleifera Abel is a highly valued woody edible oil tree, which is endemic to China. It has great economic value because C. oleifera seed oil contains a high proportion of polyunsaturated fatty acids. C. oleifera anthracnose caused by Colletotrichum fructicola, poses a serious threat to C. oleifera growth and yield and causes the benefit of the C. oleifera industry to suffer directly. The WRKY transcription factor family members have been widely characterized as vital regulators in plant response to pathogen infection. Until now, the number, type and biological function of C. oleifera WRKY genes are remains unknown. Here, we identified 90 C. oleifera WRKY members, which were distributed across 15 chromosomes. C. oleifera WRKY gene expansion was mainly attributed to segmental duplication. We performed transcriptomic analyses to verify the expression patterns of CoWRKYs between anthracnose-resistant and -susceptible cultivars of C. oleifera. These results demonstrated that multiple candidate CoWRKYs can be induced by anthracnose and provide useful clues for their functional studies. CoWRKY78, an anthracnose-induced WRKY gene, was isolated from C. oleifera. It was significantly down-regulated in anthracnose-resistant cultivars. Overexpression of CoWRKY78 in tobacco markedly reduced resistance to anthracnose than WT plants, as evidenced by more cell death, higher malonaldehyde content and reactive oxygen species (ROS), but lower activities of superoxide dismutase (SOD), peroxidase (POD), as well as phenylalanine ammonia-lyase (PAL). Furthermore, the expression of multiple stress-related genes, which are associated with ROS-homeostasis (NtSOD and NtPOD), pathogen challenge (NtPAL), and pathogen defense (NtPR1, NtNPR1, and NtPDF1.2) were altered in the CoWRKY78-overexpressing plants. These findings increase our understanding of the CoWRKY genes and lay the foundation for the exploration of anthracnose resistance mechanisms and expedite the breeding of anthracnose-resistant C. oleifera cultivars.

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Genome-wide analysis R2R3-MYB transcription factors in Xanthoceras sorbifolium Bunge and functional analysis of XsMYB30 in drought and salt stresses tolerance

Cited by 23 publications

References 55 publications

Genome-wide identification of myeloblastosis gene family and its response to cadmium stress in Ipomoea aquatica

Genome-wide identification of myeloblastosis gene family and its response to cadmium stress in Ipomoea aquatica

MbMYBC1, a M. baccata MYB transcription factor, contribute to cold and drought stress tolerance in transgenic Arabidopsis

Identification of Camellia oleifera WRKY transcription factor genes and functional characterization of CoWRKY78

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