Genome-wide association study of serum tumor markers in Southern Chinese Han population

Li, Xiukuan; Bai, Feihu; Wei, Xingwei; Jin, Tianbo; Li, Chen; Zhang, Yutian; Zhou, Xiaoli; Xie, Yufei; He, Chanyi; Lin, Qi; He, Ping; Chu, Shaoli; Ding, Yipeng

doi:10.1186/s12885-022-09236-6

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…Tumor antigens and ectopic hormones, such as CEA, AFP, NSE, CA50, CA724, CA125, etc., are currently the most often utilized tumor biomarkers in clinic [27][28][29][30][31]. CEA is a broad-spectrum tumor marker that can be used to assess e cacy, monitor disease progression, and predict prognosis in lung cancer, colorectal cancer, breast cancer, and other cancers [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker

Sun

Zhang

et al. 2023

Preprint

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Background: Exosomes have great potential as new biomarkers in liquid biopsy. However, due to the limitations of exosome extraction and component analysis procedures, further clinical applications of exosomes are hampered. Carcinoembryonic antigen (CEA) is a commonly used broad-spectrum tumor marker that is strongly expressed in a variety of malignancies. Results: In this study, CEA+ exosomes were directly separated from serum using immunomagnetic beads, and the nucleic acid to protein ultraviolet absorption ratio (NPr) of CEA+ exosomes was determined. It was found that the NPr of CEA+ exosomes in tumor group was higher than that of healthy group. We further analyzed the exosome-derived nucleic acid components using fluorescent staining and found that the concentration ratio of double-stranded DNA to protein (dsDPr) in CEA+ exosomes was also significantly different between the two groups, with a sensitivity of 100% and a specificity of 41.67% for the diagnosis of pan-cancer. The AUC of dsDPr combined with NPr was 0.87 and the ACU of dsDPr combined with CA242 could reach 0.94, showing good diagnostic performance for pan-cancer. Conclusions: This study demonstrates that the dsDPr of CEA+ exosomes can effectively distinguish exosomes derived from tumor patients and healthy individuals, which can be employed as a simple and cost-effective non-invasive screening technology to assist tumor diagnosis.

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Section: Discussionmentioning

confidence: 99%

A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker

Sun

Zhang

et al. 2023

Preprint

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show abstract

“…Tumor antigens and ectopic hormones, such as CEA, AFP, NSE, CA50, CA724, CA125, etc., are currently the most often utilized tumor biomarkers in clinic [ 32 – 36 ]. CEA overexpress in several type of tumor which associate with cell proliferation and tumor progression is a broad-spectrum tumor marker that can be used to assess efficacy, monitor disease progression, and predict prognosis in lung cancer, colorectal cancer, breast cancer, and other cancers [ 37 – 40 ].…”

Section: Discussionmentioning

confidence: 99%

A simple and available measurement of onco-sEV dsDNA to protein ratio as a potential tumor marker

Sun

Zhang

et al. 2023

BMC Cancer

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Background Small extracellular vesicles (sEVs) have great potential as new biomarkers in liquid biopsy. However, due to the limitations of sEVs extraction and component analysis procedures, further clinical applications of sEVs are hampered. Carcinoembryonic antigen (CEA) is a commonly used broad-spectrum tumor marker that is strongly expressed in a variety of malignancies. Results In this study, CEA+ sEVs were directly separated from serum using immunomagnetic beads, and the nucleic acid to protein ultraviolet absorption ratio (NPr) of CEA+ sEVs was determined. It was found that the NPr of CEA+ sEVs in tumor group was higher than that of healthy group. We further analyzed the sEV-derived nucleic acid components using fluorescent staining and found that the concentration ratio of double-stranded DNA to protein (dsDPr) in CEA+ sEVs was also significantly different between the two groups, with a sensitivity of 100% and a specificity of 41.67% for the diagnosis of pan-cancer. The AUC of dsDPr combined with NPr was 0.87 and the ACU of dsDPr combined with CA242 could reach 0.94, showing good diagnostic performance for pan-cancer. Conclusions This study demonstrates that the dsDPr of CEA+ sEVs can effectively distinguish sEVs derived from tumor patients and healthy individuals, which can be employed as a simple and cost-effective non-invasive screening technology to assist tumor diagnosis.

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Genome-wide association study of serum tumor markers in Southern Chinese Han population

Cited by 2 publications

References 41 publications

A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker

A simple and available measurement of onco-exosome dsDNA to protein ratio as a potential tumor marker

A simple and available measurement of onco-sEV dsDNA to protein ratio as a potential tumor marker

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