Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus

Abstract

Ubiquitin-conjugating enzyme (UBC) is a critical part of the ubiquitin–proteasome pathway and plays crucial roles in growth, development and abiotic stress response in plants. Although UBC genes have been detected in several plant species, characterization of this gene family at the whole-genome level has not been conducted in Brassica napus. In the present study, 200 putative BnUBCs were identified in B. napus, which were clustered into 18 subgroups based on phylogenetic analysis. BnUBCs within each subgroup showed relatively conserved gene architectures and motifs. Moreover, the gene expression patterns in various tissues as well as the identification of cis-acting regulatory elements in BnUBC promoters suggested further investigation of their potential functions in plant growth and development. Furthermore, three BnUBCs were predicted as candidate genes for regulating agronomic traits related to oil content and yield through association mapping. In conclusion, this study provided a wealth of information on the UBC family in B. napus and revealed their effects on oil content and yield, which will aid future functional research and genetic breeding of B. napus.

Wheat Grains as a Sustainable Source of Protein for Health

“…Our results exhibited some QTNs (AX-94552678, BobWhite_rep_c64315_180, and AX-109292583) located inside TraesCS5A02G429000 on 613543528-613544399 bp, a gene annotated as Ubiquitin-conjugating enzyme/RWD-like. According to different authors, Ubiquitination regulates varied plant growth and developmental processes related to protein translocation within cells, cell cycle control, photomorphogenesis responses to abiotic/biotic stress, floral development, hormone signaling, proteome homeostasis regulation, and signaling [46,47]. Interestingly, Refs.…”

UBC Gene Family Analysis in Salvia castanea and Roles of ScUBC2/5 Genes under Abiotic Stress

“…At present, the UBC gene family has been widely identified in soybean Glyma max [30], Solanum tuberosum [31], Brassica napus [32], Litchi chinensis Sonn. [33], and other plants.…”

“…Ubiquitination, a key regulator of various physiological processes including DNA repair and cell cycle control [28], is integral to multiple aspects of plant biology such as hormone responses, light signaling, embryogenesis, organogenesis, leaf aging, and defense mechanisms [29], underscoring the significant role of the UBC gene family in orchestrating the ubiquitin/proteasome pathway in plants. Research on the UBC family has been documented in many plants such as Glycine max L. [30], Solanum tuberosum L. [31], and Brassica napus L. [32], unraveling a major role of UBC genes in responding to challenges such as ultraviolet irradiation and cold stress. To better understand the adaptability of S. castanea to the extreme high-altitude environment, characterized by intense ultraviolet light and low temperatures, this study aims to perform a comprehensive analysis of ScUBC2 and ScUBC5 genes in S. castanea for their structures, functions, and expressions under stress, and their possible roles in regulating those secondary metabolites as active ingredients of this important medicinal plant.…”