Genome-wide DNA methylation analysis reveals significant impact of long-term ambient air pollution exposure on biological functions related to mitochondria and immune response

Wang, Maiqiu; Zhao, Junsheng; Wang, Yan; Mao, Ying; Zhao, Xinyi; Huang, Peng; Liu, Qiang; Ma, Yunlong; Yao, Yinghao; Yang, Zhongli; Yuan, Wenji; Cui, Wenyan; Payne, Thomas J.; Li, Ming D.

doi:10.1016/j.envpol.2020.114707

Cited by 35 publications

(12 citation statements)

References 66 publications

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“…The findings of this study suggested that exposure to single or mixture of air pollutants were positively associated with relative mtDNA-CN and these associations were counteracted by PA at certain levels. The positive associations between air pollutants and relative mtDNA-CN in our study were similar to those in several previous studies (Hou et al, 2010;Iodice et al, 2018;Wang et al, 2020a). In Milan,…”

Section: Discussionsupporting

confidence: 92%

“…For instance, exposure to particulate matter (PM) or nitrogen dioxide (NO2) is associated with an altered mtDNA-CN via leading to excessive production of reactive oxygen species in the mitochondria and subsequently damage the structure of the mitochondria (Li et al, 2003;Bai et al, 2018;Wang et al, 2020b). Several studies show inconsistent results on mtDNA-CN alteration in relation to short-or long-term exposure to air pollution (Iodice et al, 2018;Wang et al, 2020a;Wang et al, 2020b). The differences in these studies may be due to different levels and components of air pollution, and studies' location regions and sample size.…”

Section: Introductionmentioning

confidence: 99%

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Physical activity counteracted associations of exposure to mixture of air pollutants with mitochondrial DNA copy number among rural Chinese adults

Pan

et al. 2021

Chemosphere

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Physical activity counteracted associations of exposure to mixture of air pollutants with mitochondrial DNA copy number among rural Chinese adults

Pan

et al. 2021

Chemosphere

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“…So this may be why air pollution raises the risk of cardiovascular disease. Previous studies have focused on the methylation of mitochondrial genes in peripheral blood leukocytes, but there is a lack of studies on the relationship between the methylation of mitochondrial genes in a single type of cell and acute air pollution ( Byun et al, 2013 ; Wang et al, 2020a ). The mtCO2 site two methylations of participants in the SJZ period were significantly higher than that in other periods.…”

Section: Discussionmentioning

confidence: 99%

Platelet Mitochondrial DNA Methylation as Epigenetic Biomarker of Short-Term Air Pollution Exposure in Healthy Subjects

Sun

Zhang

et al. 2022

Front. Mol. Biosci.

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Air pollution exposure is now considered a growing concern for global public health. RNA or DNA methylation changes caused by air pollution may be related to the development of cardiovascular disease. To investigate the early biomarkers of air pollution exposure, a panel study of eight college students recorded after a business trip from Qingdao to Shijiazhuang and back to Qingdao was performed in this work. The concentration of PM2.5, PM10, SO2, NO2, and CO in Shijiazhuang was higher than that in Qingdao during the study period. The platelet count was positively correlated with air pollutants of 0–6 day moving averages (βPM2.5 = 88.90; βPM10 = 61.83; βSO2 = 41.13; βNO2 = 57.70; βCO = 62.99, respectively, for an IQR increased). Additionally, internal dose biomarkers 2-OHNa, 1-OHNa, 2-OHFlu, 2,3-OHPhe, and ∑PAHs were also significantly associated with platelet count in participants. Furthermore, PM2.5 and PM10 are positively linked with methylation of one CpG site at platelet mitochondrial gene CO2 (PM2.5 = 0.47; PM10 = 0.25, respectively, for an IQR increase). Both platelet counts and methylation levels returned to their pre-exposure levels after leaving the highly contaminated area. In short, this study investigated the relationship between platelet properties and air pollution exposure, revealing that short-term exposure to air pollution might increase the risk of thrombosis. Our research suggests that platelet count and mitochondrial DNA methylation of mtCO2 site 2 in platelets from healthy adults may be the novel biomarker for acute exposure to air pollution.

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“…Paired tumor and adjacent non-tumor tissue samples from 33 HCC patients were subjected to WGBS on the Illumina X Ten platform with the procedures described in our previous WGBS paper ( 29 ). Briefly, a 200-ng genomic DNA sample was sheared to about 300-bp fragments by sonication.…”

Section: Methodsmentioning

confidence: 99%

Integrative Analysis of Epigenome and Transcriptome Data Reveals Aberrantly Methylated Promoters and Enhancers in Hepatocellular Carcinoma

Huang

Han

et al. 2021

Front. Oncol.

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DNA methylation is a key transcription regulator, whose aberration was ubiquitous and important in most cancers including hepatocellular carcinoma (HCC). Whole-genome bisulfite sequencing (WGBS) was conducted for comparison of DNA methylation in tumor and adjacent tissues from 33 HCC patients, accompanying RNA-seq to determine differentially methylated region-associated, differentially expressed genes (DMR-DEGs), which were independently replicated in the TCGA-LIHC cohort and experimentally validated via 5-aza-2-deoxycytidine (5-azadC) demethylation. A total of 9,867,700 CpG sites showed significantly differential methylation in HCC. Integrations of mRNA-seq, histone ChIP-seq, and WGBS data identified 611 high-confidence DMR-DEGs. Enrichment analysis demonstrated activation of multiple molecular pathways related to cell cycle and DNA repair, accompanying repression of several critical metabolism pathways such as tyrosine and monocarboxylic acid metabolism. In TCGA-LIHC, we replicated about 53% of identified DMR-DEGs and highlighted the prognostic significance of combinations of methylation and expression of nine DMR-DEGs, which were more efficient prognostic biomarkers than considering either type of data alone. Finally, we validated 22/23 (95.7%) DMR-DEGs in 5-azadC-treated LO2 and/or HepG2 cells. In conclusion, integration of epigenome and transcriptome data depicted activation of multiple pivotal cell cycle-related pathways and repression of several metabolic pathways triggered by aberrant DNA methylation of promoters and enhancers in HCC.

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Genome-wide DNA methylation analysis reveals significant impact of long-term ambient air pollution exposure on biological functions related to mitochondria and immune response

Cited by 35 publications

References 66 publications

Physical activity counteracted associations of exposure to mixture of air pollutants with mitochondrial DNA copy number among rural Chinese adults

Physical activity counteracted associations of exposure to mixture of air pollutants with mitochondrial DNA copy number among rural Chinese adults

Platelet Mitochondrial DNA Methylation as Epigenetic Biomarker of Short-Term Air Pollution Exposure in Healthy Subjects

Integrative Analysis of Epigenome and Transcriptome Data Reveals Aberrantly Methylated Promoters and Enhancers in Hepatocellular Carcinoma

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