Genome-wide expression analysis of a rice mutant line under salt stress

Abstract

ABSTRACT. Salinity is a major environmental stress to plants. In this study, the ability of plants to tolerate salt was investigated by studying growth, physiological characteristics, and expression levels of genes related to the salt-stress response in the salt-tolerant rice mutant (Till-II-877), which was derived from γ-ray irradiation. Compared to plants grown under normal conditions, the height and root length of wild type (WT) were reduced by approximately 40 and 29% following exposure to salt stress for 3 weeks, whereas Till-II-877 line showed 29 and 23% reductions in plant height and root length, respectively. No significant changes were observed in total chlorophyll content, and the malondialdehyde content of the mutant increased less than that of the WT under salt treatment. Gene expression was compared between the WT and mutant lines using microarray analysis. An unbiased analysis of the gene expression datasets allowed us to identify the pathways involved in salt-stress responses. Among the most significantly affected pathways, changes in gene expression were observed in α-linolenic acid and linoleic acid metabolism (in lipid metabolism), fructose and mannose metabolism and glycolysis-gluconeogenesis (in carbohydrate metabolism), cysteine and methionine metabolism (in amino acid metabolism), and carbon fixation (in the energy metabolism of photosynthetic organisms) under salt stress. These results show that the differential response of plants subjected to salt stress was due to changes in multiple metabolic pathways. These findings increase our understanding of the effects of salt stress in rice and may aid in the development of salt-tolerant rice cultivars.

Comparative analysis of salt-induced changes in the leaves proteome of two contrasting Jatropha curcas genotypes

“…Salt-stressed plants generally have reduced translation of enzymes related to this carbohydrate metabolism [43,51]. This contradicts our results; however, some studied gene expression have shown greater induction of genes related to pyruvate metabolism pathways, such as pyruvate kinase (PQ), in Oryza sativa [52] and Saccharum spp. [53] submitted to abiotic stresses, seemly as observed in this study in the tolerant-like J. curcas genotype (spot 282, Table 1).…”

Physiological and Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.) Varieties to PEG-Induced Osmotic Stress

“…Of these, the increased accumulation of 6-phosphofructokinase (gi|358347084) and NAD-dependent aldehyde dehydrogenase (ALDH) family protein (gi|657394832), involved in the glycolysis and gluconeogenesis pathway, could contribute to enhance drought-tolerance of alfalfa, possibly through promoting carbohydrate metabolism and aldehyde detoxification ( Končitíková et al, 2015 ; Yao and Wu, 2016 ). Lee et al (2016) have proposed that genes implicated in fructose and mannose metabolism were up-regulated by salt stress in a rice mutant line. Consistent with this finding, we observed the increased abundance of L -idonate 5-dehydrogenase (gi|358345353), involved in fructose and mannose metabolism, indicating that the induction of this protein is associated with the drought tolerance in Longzhong under PEG stress.…”

Isobaric tags for relative and absolute quantification‐based proteomic analysis of Puccinellia tenuiflora inoculated with arbuscular mycorrhizal fungi reveal stress response mechanisms in alkali‐degraded soil

“…Moreover, Cys and Met are precursors of S‐adenosylmethionine, hydrogen sulfide, taurine, and glutathione. These products alleviate oxidative stress induced by various oxidants (Bin, Huang, & Zhou, ; Lee et al, ). In our study, several proteins involved in Cys and Met metabolism were up‐regulated after AMF inoculation under AS (AS‐AM vs. AS analysis; Table S5 and Figure ).…”