Genome-wide identification and abiotic stress responses of DGK gene family in maize

Comprehensive Genomic Analysis and Expression Profiling of Diacylglycerol Kinase (DGK) Gene Family in Soybean (Glycine max) under Abiotic Stresses

“…Whereas GmDGK s in cluster III were slightly regulated, we noted that the GmDGK s in clusters I and II were strongly upregulated in both tissues with a maximum fold change of 4.3 for GmDGK2 in leaf tissues at 9 h of stress application (Figure 7). A similar study was conducted on maize ( Zea mays ), and it was reported that the relative expression levels of ZmDGK7 were prominent in many tissues under salt stress treatments [16]. These results indicated that, in Glycine max , various isoforms of GmDGK s could react differentially to several abiotic stresses, and that the upregulation of all GmDGK s under PEG, saline, and alkali treatments suggested a specific role of GmDGK s in abiotic stress responses.…”

“…The alignment revealed that, except for GmDGK2 , GmDGK8 , and GmDGK9 , almost all GmDGKs conserved the ATP-binding motif, represented by a sequence of GXGXXG in their catalytic domain (DGKc), as shown in the marked area of Figure 2A. As in other studied plants, cluster I also featured the DAG/PE-binding domain (C1 domain, PF00130), which harbors the sequence HX14CX2CX16–22 CX2CX4HX2CX7C and the DAG/PE-binding domain HX18CX2CX16CX2CX4HX2CX11C [16]. This DAG/PE-binding domain holds a preserved 15 amino-acid (aa) extension, and this domain was, therefore, named the “extended cysteine-rich domain” (extCRD) [17].…”

“…In silico identification was used for the functional predictions of the DGK gene family in Arabidopsis , apple, rice, and maize, and the primer background was used for further studies on the DGK gene functional analysis in these plants under drought stress. The upregulation of DGK genes in maize under drought, salt, and cold stresses and their obvious downregulation during salt stress treatment [16] first revealed DGK activity under drought stress. Later, researchers discovered the modulating role of DGK genes in root development under salt stress in Arabidopsis [13], the higher response of the DGK genes under drought stress treatment in apple [14], the expression of both AtDGK1 and AtDGK2 in the Arabidopsis cold-responsive transcriptome [28], and several repressions observed on DGK genes under various stresses in other plants (barley and wheat).…”

Genome-wide identification and comparative analysis of diacylglycerol kinase (DGK) gene family and their expression profiling in Brassica napus under abiotic stress

“…S2) indicate that the first and second DAG/PE-binding domains harbor the sequences HX 14 CX 2 CX 19 ~ 22 CX 2 CX 4 HX 2 CX 7 C and HX 18 CX 2 CX 16 CX 2 CX 4 HX 2 CX 11 C, respectively. The two extremely conserved C 6 /H 2 cores were also observed in cluster 1 DGKs of other plants, such as soybean [ 24 ], apple [ 4 ], and maize [ 22 ]. Moreover, the alignment revealed that, except for the orthologs of AtDGK2 , almost all DGKs possess a presumed ATP-binding motif with a GXGXXG consensus sequence in their catalytic domain (DGKc), where G represents glycine and X represents any other amino acid (Additional file 3 : Fig.…”

“…Therefore, the relative flux of TAG synthesis from de-novo-synthesized or PC-derived DAG can greatly affect the final seed oil content. The DGK gene family has been widely characterized in the context of plant stress tolerance, including tomato ( Solanum lycopersicum , SlDGK ) [ 20 ], Arabidopsis thaliana ( AtDGK ) [ 21 ], maize ( Zea mays , ZmDGK ) [ 22 ], rice ( Oryza sativa , OsDGK ) [ 23 ], apple ( Malus domestica , MdDGK ) [ 4 ], and soybean ( Glycine max , GmDGK ) [ 24 ]. However, the genomic analysis of the DGK gene family in Brassica species has not been reported.…”