Genome-wide identification and characterization of the aquaporin gene family in Medicago truncatula

Min, Xueyang; Wu, Honglin; Zhang, Zhengshe; Wei, Xingyi; Jin, Xiaoyu; Ndayambaza, Boniface; Wang, Yanrong; Liu, Wenxian

doi:10.1007/s13562-018-0484-4

Cited by 22 publications

(19 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the high similarity found in these amino acid sequences in MtNIP5;1 and AtNIP5;1 (Fig. 2c) provides further evidence in support of a comparable permeability of non-aqua substrates, such as boric acid [36,40].…”

Section: Discussionsupporting

confidence: 56%

See 1 more Smart Citation

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

2020

View full text Add to dashboard Cite

Background Legumes comprise important crops that offer major agronomic benefits, including the capacity of establishing symbiosis with rhizobia, fixing atmospheric N2. It has been proven that legumes are particularly susceptible to boron (B) stress, which leads to important yield penalties. Boron (B) deficiency or toxicity in plants causes the inhibition of growth and an altered development. Under such conditions, the participation of two distinct protein families (the major intrinsic protein family MIP and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress. However, in legumes, little is known about the transport mechanisms responsible for B uptake and distribution, especially under deficiency. Results A Medicago truncatula protein, MtNIP5;1 (Medtr1g097840) (homologous to the Arabidopsis thaliana AtNIP5;1) was identified as a novel legume B transporter involved in B uptake under deficiency. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, the partial complementation of the nip5;1–1 A. thaliana mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter in this legume model plant. Conclusions The results here presented support a functional role of MtNIP5;1 in B uptake under deficiency and provides new insights into B transport mechanisms in legume species.

show abstract

Section: Discussionsupporting

confidence: 56%

“…2c). This supports a shared role between these two proteins transporting boric acid across the plasma membrane [36,39,40]..…”

Section: Identification Of Atnip5;1 Homologs In M Truncatulasupporting

confidence: 58%

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

2020

View full text Add to dashboard Cite

show abstract

“…The ubiquitous and high expression of PIP and TIP in various organs and tissues is widely reported [18,81,82]. In this regard and whatever the stress, reduced expression of these members is generally expected to prevent loss of metabolic energy in situations of severe stress and/or to prevent loss of water from challenged organs or tissues to the hypertonic surrounding environment.…”

Section: Functional Consideration Of Oleuraqpmentioning

confidence: 99%

Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)

Faize

Fumanal

Luque

et al. 2020

IJMS

View full text Add to dashboard Cite

Cellular aquaporin water channels (AQPs) constitute a large family of transmembrane proteins present throughout all kingdoms of life, playing important roles in the uptake of water and many solutes across the membranes. In olive trees, AQP diversity, protein features and their biological functions are still largely unknown. This study focuses on the structure and functional and evolution diversity of AQP subfamilies in two olive trees, the wild species Olea europaea var. sylvestris (OeuAQPs) and the domesticated species Olea europaea cv. Picual (OleurAQPs), and describes their involvement in different physiological processes of early plantlet development and in biotic and abiotic stress tolerance in the domesticated species. A scan of genomes from the wild and domesticated olive species revealed the presence of 52 and 79 genes encoding full-length AQP sequences, respectively. Cross-genera phylogenetic analysis with orthologous clustered OleaAQPs into five established subfamilies: PIP, TIP, NIP, SIP, and XIP. Subsequently, gene structures, protein motifs, substrate specificities and cellular localizations of the full length OleaAQPs were predicted. Functional prediction based on the NPA motif, ar/R selectivity filter, Froger’s and specificity-determining positions suggested differences in substrate specificities of Olea AQPs. Expression analysis of the OleurAQP genes indicates that some genes are tissue-specific, whereas few others show differential expressions at different developmental stages and in response to various biotic and abiotic stresses. The current study presents the first detailed genome-wide analysis of the AQP gene family in olive trees and it provides valuable information for further functional analysis to infer the role of AQP in the adaptation of olive trees in diverse environmental conditions in order to help the genetic improvement of domesticated olive trees.

show abstract

“…2C) supporting a shared role between these two proteins transporting boric acid across the plasma membrane. (36,39,40).…”

Section: Identi Cation Of Atnip5;1 Homologs In M Truncatulamentioning

confidence: 99%

“…However, B transport has been barely studied in legumes and the MIP protein superfamily seems to harbor putative B transporter candidates. In the model plant Medicago truncatula Gaertn, this family comprises 46 MIPs, 18 of them belonging to the NIP family (36). However, only one B transporter (a NIP protein) has been characterized so far (37), and none has been associated with B transport under B de cient conditions.…”

Section: Introductionmentioning

confidence: 99%

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Granado‐Rodríguez

Bolaños

Reguera

2020

Preprint

View full text Add to dashboard Cite

Background: Boron (B) deficiency or toxicity in soils can cause the inhibition of growth and an altered development in plants. In such conditions, the participation of two distinct protein families (the aquaporin family Major intrinsic proteins (MIPs) and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress. Legumes comprise important crops that offer major agronomic benefits, including the capacity of establishing symbiosis with rhizobia fixing atmospheric N2 and for being important food protein sources. It has been proven that legumes are susceptible to B stress, which leads to important yield penalties. However, little is known about the transport mechanisms responsible for B uptake and distribution in legumes, especially under deficiency.Results: A Medicago truncatula protein, MtNIP5;1 (Medtr1g097840) (homologous to the Arabidopsis thaliana AtNIP5;1) was identified as a novel legume B transporter involved in B uptake under deficiency. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, a partial complementation of the nip5;1 A. thaliana mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter in this legume model plant. Conclusions: The results here presented support a functional role of MtNIP5;1 in B uptake under deficiency and provides new insights into this micronutrient transport mechanisms in legume species.

show abstract

Genome-wide identification and characterization of the aquaporin gene family in Medicago truncatula

Cited by 22 publications

References 57 publications

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Contact Info

Product

Resources

About