Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)

Li, Wen; Xia, Xiao-Cong; Han, Lihong; Ni, Ping; Yan, Jing-Qiu; Tao, Miao; Huang, Geng-Qing; Li, Xuebao

doi:10.1038/s41598-017-03155-4

Cited by 27 publications

(21 citation statements)

References 64 publications

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“…The number and length of exon and intron showed great variations in CsJAZ genes. This finding was consistent with the JAZ genes of other plants, such as upland cotton and wheat [10,15]. The structure variations of JAZ genes might be caused by structural divergence mechanisms, such as the insertion or deletion of the exons and introns proposed by Xu et al [32].…”

Section: Discussionsupporting

confidence: 89%

“…In wheat, TaJAZ6 and 13 were specifically expressed in root, and the expression level of TaJAZ3 in root tissues was as high as that in stamen [10]. GhJAZ1-A/D, GhJAZ3-A/D, GhJAZ5-A/D, and GhJAZ13-A/D in the upland cotton also showed higher expression in roots than in stems or leaves [15]. These results indicated that CsJAZ genes might exert certain functions as SmJAZ1 and SmJAZ2 [36] in roots and maintain the normal development of tea plants under normal conditions.…”

Section: Discussionmentioning

confidence: 92%

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Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

Shen

Zou

Xing

et al. 2020

IJMS

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JAZ (Jasmonate ZIM-domain) proteins play pervasive roles in plant development and defense reaction. However, limited information is known about the JAZ family in Camellia sinensis. In this study, 12 non-redundant JAZ genes were identified from the tea plant genome database. Phylogenetic analysis showed that the 12 JAZ proteins belong to three groups. The cis-elements in promoters of CsJAZ genes and CsJAZ proteins interaction networks were also analyzed. Quantitative RT–PCR analysis showed that 7 CsJAZ genes were preferentially expressed in roots. Furthermore, the CsJAZ expressions were differentially induced by cold, heat, polyethylene glycol (PEG), methyl jasmonate (MeJA), and gibberellin (GA) stimuli. The Pearson correlations analysis based on expression levels showed that the CsJAZ gene pairs were differentially expressed under different stresses, indicating that CsJAZs might exhibit synergistic effects in response to various stresses. Subcellular localization assay demonstrated that CsJAZ3, CsJAZ10, and CsJAZ11 fused proteins were localized in the cell nucleus. Additionally, the overexpression of CsJAZ3, CsJAZ10, and CsJAZ11 in E. coli enhanced the growth of recombinant cells under abiotic stresses. In summary, this study will facilitate the understanding of the CsJAZ family in Camellia sinensis and provide new insights into the molecular mechanism of tea plant response to abiotic stresses and hormonal stimuli.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 92%

Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

Shen

Zou

Xing

et al. 2020

IJMS

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“…The released MYC in turn activates the transcription of JAZ (Zhang et al, 2015). The JAZ proteins are a subgroup of the TIFY family, characterized by the N-terminal located ZIM domain and the C-terminal located Jas domain (Thines et al, 2007; Melotto et al, 2008; Li et al, 2017). The ZIM domain, which consists of 30 amino acids with a highly conserved TIFY motif (TIF[F/Y]XG), is required for the formation of homo- and hetero-dimers within the JAZ proteins (Vanholme et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Hevea brasiliensis)

et al. 2019

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Jasmonate signaling plays a vital role in the regulation of secondary laticifer differentiation and natural rubber biosynthesis in Hevea brasiliensis . Jasmonate ZIM-domain (JAZ) proteins are the master regulators of jasmonate signaling. Although several JAZs have been reported in the laticifer cells of H. brasiliensis , the genome-wide screening of HbJAZ members has not yet been explored. In the present study, 18 HbJAZs were identified based on the recent H. brasiliensis genome. Phylogenetic construction revealed that the HbJAZs were clustered into five subgroups and that members within the same subgroup shared highly conserved gene structures and protein motifs. Cis -element analysis of HbJAZ promoters suggested the presence of hormone, stress and development-related cis -elements. HbJAZ1.0, HbJAZ2.0, and HbJAZ5.0 interacted with CORONATINE INSENSITIVE1 (COI1) in the presence of coronatine (COR, a JA mimic). HbJAZ1.0, HbJAZ2.0, HbJAZ5.0, and HbJAZ12.0 could also interact with each other. Of the 18 HbJAZs , transcripts of 15 HbJAZs were present in the vascular cambium region except for that of HbJAZ7.0 , HbJAZ8.0d , and HbJAZ13.0 . Fourteen of the 15 HbJAZs were significantly up-regulated upon COR treatment. The transcripts of three genes that were absent from vascular cambium region were also absent from the latex. Among the 15 HbJAZs in the latex, the expression patterns of 13 HbJAZs were different between the tapping and ethrel treatments. Eight of the 14 COR-up-regulated HbJAZs in the vascular cambium region were also activated by tapping in latex. Of the eight tapping-activated HbJAZs , 5 HbJAZs were repressed by ethrel application. Based on the computational analyses and gene expression patterns described in this study, the HbJAZ5.0 and HbJAZ10.0b may be associated with laticifer differentiation while the HbJAZ8.0b is a negative regulator for natural rubber biosynthesis in H. brasiliensis .

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“…Cis-elements located in the promoter region of genes play key roles in the developmental and environmental regulation of gene expression [42]. According to cis-element analysis, the promoter regions of Gh4CL genes possess elements related to stress responses, such as ABRE, TC-rich, LTR, MBS, TGA-element, TCA-element, CGTCA-motif and TGACG-motif [43][44][45][46], suggesting a potential role of the Gh4CL genes with these cis-element(s) in tolerance of stresses, such as drought, salt, heat, ABA, and low temperature.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Sun

Xiong

Zhang

et al. 2019

Preprint

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Background The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses have been studied in plants, however, limite is known about the upland cotton 4CL genes and their roles in response to drought stress. ResultsIn this study, we performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis -elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 were up-regulated under the PEG simulated drought stress conditions, including Gh4CL7 . Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7 -silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ). Arabidopsis lines overexpressing Gh4CL7 , however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H 2 O 2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7 -silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a lower and higher lignin content, respectively. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion We comprehensively identified 4CL family genes in upland cotton and provide valuable information on the evolutionary relationship. Gh4CL7 gene is involved in lignin biosynthesis and play a positively role in reponsing drought stress.

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Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)

Cited by 27 publications

References 64 publications

Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Hevea brasiliensis)

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

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