Genome-Wide Identification and Characterization of Short-Chain Dehydrogenase/Reductase (SDR) Gene Family in Medicago truncatula

Abstract

Short-chain dehydrogenase/reductase (SDR) belongs to the NAD(P)(H)-dependent oxidoreductase superfamily. Limited investigations reveal that SDRs participate in diverse metabolisms. A genome-wide identification of the SDR gene family in M. truncatula was conducted. A total of 213 MtSDR genes were identified, and they were distributed on all chromosomes unevenly. MtSDR proteins were categorized into seven subgroups based on phylogenetic analysis and three types including ‘classic’, ‘extended’, and ‘atypical’, depending on the cofactor-binding site and active site. Analysis of the data from M. truncatula Gene Expression Atlas (MtGEA) showed that above half of MtSDRs were expressed in at least one organ, and lots of MtSDRs had a preference in a tissue-specific expression. The cis-acting element responsive to plant hormones (salicylic acid, ABA, auxin, MeJA, and gibberellin) and stresses were found in the promoter of some MtSDRs. Many genes of MtSDR7C, MtSDR65C, MtSDR110C, MtSDR114C, and MtSDR108E families were responsive to drought, salt, and cold. The study provides useful information for further investigation on biological functions of MtSDRs, especially in abiotic stress adaptation, in the future.

Silicon-Induced Morphological, Biochemical and Molecular Regulation in Phoenix dactylifera L. under Low-Temperature Stress

“…The current findings show that the supplementation of Si significantly triggered the transcript accumulation of SDR at low-temperature stress (5 °C) in date palms, therefore, suggesting the stress-ameliorative role of Si supplementation. Similar upregulations of SDR genes and proteins are reported for chilling and low-temperature stress in corn and Medicago truncatula [ 66 , 67 ]. Moreover, SRC2 has been described as an activator of the calcium-dependent activation of RBOHF that mediates ROS production and may play a role in low-temperature stress responses [ 68 ].…”

Plastidial Expression of 3β-Hydroxysteroid Dehydrogenase and Progesterone 5β-Reductase Genes Confer Enhanced Salt Tolerance in Tobacco

“…Therefore, keeping in consideration such inducible factors for the regulation of the 3β-HSD , P5βR1 and P5βR2 genes we hypothesized that perhaps plastidial expression of these genes would confer salt tolerance in the present research. Further, our hypothesis was strengthened as some orthologous gene from SDR family were induced due to salt stress in bacteria [ 34 ] or plant [ 3 ] and the recent findings further strengthening our hypothesis [ 35 , 36 ].…”

Genome-Wide Identification of Halogenated acid dehalogenase Genes in Medicago

“…In this study, proteins containing the HAD domain were searched using hidden Markov models of HAD and the speci c species model was constructed using the obtained HAD domains. Among the conserved motifs of HAD proteins, a mutation of aspartic acid in motif I would result in a signi cant reduction or even loss of enzyme activity [30] . Therefore, the proteins identi ed by MEME Suite software were further manually screened, resulting in 24 MtrHAD and 29 MsaHAD genes nally found in M. truncatula and M. sativa, respectively (Table S1).…”