Genome-wide identification and characterization of the TPS gene family in wheat (Triticum aestivum L.) and expression analysis in response to aphid damage

Zhao, Lei; Zhao, Xiaojing; Francis, Frédéric; Liu, Yong

doi:10.1007/s11738-021-03236-y

Cited by 8 publications

(6 citation statements)

References 74 publications

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“…Plants possess a variety of TPSs, which are responsible for the biosynthesis of terpenoids that function in growth, development, and stress responses. In this study, 196 TPS unigenes were identified from M. micrantha transcriptome, which was comparable with those reported in some angiosperms 42 . The phylogenetic tree showed that M. micrantha TPS sequences were divided into TPS-a, TPS-b, TPS-c, TPS-f, and TPS-g subfamilies, which was congruent with the result of phylogenetic analysis of TPS in angiosperms 43 .…”

Section: Discussionsupporting

confidence: 87%

“…TPS-c and TPS-e/f subfamilies contain sesqui-, mono-, and other diterpene synthases 43 . TPS-a, TPS-b, and TPS-g are angiosperm-specific subfamilies, comprising genes of specialized sesqui-, mono-, and diterpene biosynthesis, which participate in response to insect damage and abiotic stress 42 , 45 . Mikania micrantha TPS-a and TPS-b subfamilies were mainly highly expressed in the root, leaf, or flower, suggesting their significant implications for root, leaf, and flower.…”

Section: Discussionmentioning

confidence: 99%

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Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Ruan

Wang

et al. 2022

Sci Rep

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Mikania micrantha is a notorious invasive weed that has caused huge economic loss and negative ecological consequences in invaded areas. This species can adapt well to invasive environments with various stress factors. The identification of gene families and functional pathways related to environmental adaptability is lack in M. micrantha at the multi-organ full-length transcriptome level. In this study, we sequenced the transcriptomes of five M. micrantha organs using PacBio single-molecule real-time sequencing and Illumina RNA sequencing technologies. Based on the transcriptome data, full-length transcripts were captured and gene expression patterns among the five organs were analyzed. KEGG enrichment analysis of genes with higher expression indicated their special roles in environmental stress response and adversity adaptation in the various five organs. The gene families and pathways related to biotic and abiotic factors, including terpene synthases, glutathione S-transferases, antioxidant defense system, and terpenoid biosynthesis pathway, were characterized. The expression levels of most differentially expressed genes in the antioxidant defense system and terpenoid biosynthesis pathway were higher in root, stem, and leaf than in the other two organs, suggesting that root, stem, and leaf have strong ability to respond to adverse stresses and form the important organs of terpenoid synthesis and accumulation. Additionally, a large number of transcription factors and alternative splicing events were predicted. This study provides a comprehensive transcriptome resource for M. micrantha, and our findings facilitate further research on the adaptive evolution and functional genomics of this species.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Ruan

Wang

et al. 2022

Sci Rep

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“…Moreover, the YxxxK motif displayes the catalytic center [ 35 ] and remains responsible for the predilection for NADP(H) over NAD(H) [ 36 , 37 ]. Finally, each protein sequences have one or two or all of these conserved domains are belong to the terpene synthase family [ 3 , 4 , 11 , 38 – 40 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of a Novel NAD+-dependent farnesol dehydrogenase SoFLDH gene involved in sesquiterpenoid synthases from Salvia officinalis

et al. 2022

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Salvia officinalis is one of the most important medicinal and aromatic plants in terms of nutritional and medicinal value because it contains a variety of vital active ingredients. Terpenoid compounds, particularly monoterpenes (C10) and sesquiterpenes, are the most important and abundant among these active substances (C15). Terpenes play a variety of roles and have beneficial biological properties in plants. With these considerations, the current study sought to clone theNAD+-dependent farnesol dehydrogenase (SoFLDH, EC: 1.1.1.354) gene from S. officinalis. Functional analysis revealed that, SoFLDH has an open reading frame of 2,580 base pairs that encodes 860 amino acids.SoFLDH has two conserved domains and four types of highly conserved motifs: YxxxK, RXR, RR (X8) W, TGxxGhaG. However, SoFLDH was cloned from Salvia officinalis leaves and functionally overexpressed in Arabidopsis thaliana to investigate its role in sesquiterpenoid synthases. In comparison to the transgenic plants, the wild-type plants showed a slight delay in growth and flowering formation. To this end, a gas chromatography-mass spectrometry analysis revealed that SoFLDH transgenic plants were responsible for numerous forms of terpene synthesis, particularly sesquiterpene. These results provide a base for further investigation on SoFLDH gene role and elucidating the regulatory mechanisms for sesquiterpene synthesis in S. offcinalis. And our study paves the way for the future metabolic engineering of the biosynthesis of useful terpene compounds in S. offcinalis.

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“…The putative function of SgCINS gene was initially predicted based on the sequence alignment with well-known other TPS sequences and conserved motifs from Lamiaceae family and other plants. Based on these predictions, SgCINS protein has various motifs such as RR (X8) W (residues 55-65), LYEAS (residues 194-199), RXR (residues 311-313), aspartate-rich-DDxxD (residues 348-352), and GTLxEL (residues 355-360) region, which are dominant at similar TPS genes (Zhao et al, 2021;Abbas et al, 2019;Su-Fang et al, 2014;Degenhardt et al, 2009;Ali et al, 2017Ali et al, , 2018; Supplementary Figure 1). Compared to other TPS genes, SgCINS has six domains that were specified by the InterPro protein sequence analysis and classification (InterPro 10 ) database.…”

Section: Bioinformatics Analysis Of Sgcins Genementioning

confidence: 99%

“…So, SgCINS protein has six TPS family domains at different amino acid positions such as terpene cyclases, class 1, plant (IPR044814: from 55 to 369); TPS, N-terminal domain (IPR001906: from 65 to 242); TPS, metal-binding domain (IPR005630: from 273 to 386); terpenoid cyclases/protein prenyltransferase alphaalpha toroid (IPR008930: from 64 to 267); isoprenoid synthase domain superfamily (IPT08949: from 269 to 409) and TPS, and N-terminal domain superfamily (IPR036965: from 65 to 282) (Figure 2). Finally, each protein sequence has one or two or all of these conserved domains belonging to the TPS family (Zhao et al, 2021;Abbas et al, 2019;Su-Fang et al, 2014;Degenhardt et al, 2009;Ali et al, 2017Ali et al, , 2018.…”

Section: Bioinformatics Analysis Of Sgcins Genementioning

confidence: 99%

Cloning and Characterization of 1,8-Cineole Synthase (SgCINS) Gene From the Leaves of Salvia guaranitica Plant

et al. 2022

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Monoterpenes are one of the most common groups belonging to the terpenoid family, with a C10 structure comprising of two isoprene units. Most of monoterpenes are volatile plant compounds, and they act as signaling molecules between plants and the environment, particularly as defensive compounds against herbivores and pathogens. In this study, 1,8-cineole synthase (SgCINS) gene was identified and cloned from the leaves of Salvia guaranitica plant. To examine the role of SgCINS in insect resistance, we transformed and expressed this gene into tobacco leaves. The metabolic analysis revealed that the production of various types and amount of terpenoid was increased and decreased in SgCINS overexpression and control lines, respectively, suggesting that overexpressing SgCINS in transgenic tobacco plants lead to an increase in the production of various types of terpenoids and other phytochemical compounds. These results indicated why transgenic tobacco was highly resistant against cotton worm than the highly susceptible control plants. Our results demonstrate that the SgCINS gene can play an important role in plants against cotton worm insect attack, and pave the way for using terpenoids genes for improving resistance to insect attack in higher plants.

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Genome-wide identification and characterization of the TPS gene family in wheat (Triticum aestivum L.) and expression analysis in response to aphid damage

Cited by 8 publications

References 74 publications

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Full-length transcriptome analysis of multiple organs and identification of adaptive genes and pathways in Mikania micrantha

Molecular characterization of a Novel NAD+-dependent farnesol dehydrogenase SoFLDH gene involved in sesquiterpenoid synthases from Salvia officinalis

Cloning and Characterization of 1,8-Cineole Synthase (SgCINS) Gene From the Leaves of Salvia guaranitica Plant

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