Genome-wide identification and comparison of differentially expressed profiles of miRNAs and lncRNAs with associated ceRNA networks in the gonads of Chinese soft-shelled turtle, Pelodiscus sinensis

Ma, Xiao; Cen, Shuangshuang; Wang, Luming; Zhang, Chao; Wu, Limin; Xue, Tian; Wu, Qing; Li, Xuejun; Wang, Xiaoqing

doi:10.1186/s12864-020-06826-1

Cited by 16 publications

(12 citation statements)

References 70 publications

(79 reference statements)

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“…It has also been reported that MALAT1 plays an important role in DM and its complications. 54 , 55 Li et al 35 found the expression of MALAT1 increased and that of miR-23c decreased in rats with streptozotocin-induced DM and HG-induced HK-2 renal tubular epithelial cells. Downregulation of MALAT1 expression or upregulating miR-23c expression might inhibit pyroptosis, with decreased expression of NLRP3, caspase-1 and IL-1β, suggesting that downregulation of MALAT1 inhibits pyroptosis induced by HG.…”

Section: Discussionmentioning

confidence: 99%

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Atorvastatin Regulates MALAT1/miR-200c/NRF2 Activity to Protect Against Podocyte Pyroptosis Induced by High Glucose

Zuo¹,

Chen²,

He³

et al. 2021

DMSO

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Background Diabetic nephropathy (DN) is one of the main complications of diabetes mellitus (DM), which leads to the long-term loss of kidney functions. Long noncoding RNAs (LncRNAs) can alleviate DN by interacting with microRNAs (miRNAs). In this work, we aimed to explore the effects of the MALAT1/miR-200c/NRF2 regulatory axis on the pyroptosis and oxidative stress (Oxidative stress, OS) of renal podocytes in high glucose (HG) environment and whether the lipid-lowering drug atorvastatin (AT) can relieve renal OS through this approach. Methods MPC-5, a mouse podocyte cell line, was induced by HG as a cell model. The protein expressions of caspase-1, GSDMD, NLRP3, NRF2, etc. were detected by Western blotting and immunofluorescence, and the mRNA level of caspase-1, GSDMD, NLRP3, NRF2, MALAT1, miR-200c was tested by qRT-PCR. The cell pyroptosis of podocytes treated with AT was verified by CCK-8 or flow cytometry. The levels of Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) were measured by spectrophotometer, respectively. Results The caspase-1 was upregulated in time-dependent manner and got the peak at 48 h and 30 mmol/L respectively in MPC-5 cells treated with HG. Further, the expression of GSDMD, MALAT1 and miR-200c were increased, while the level of NRF2, HO-1, OS-related indicators, were decreased simultaneously. Knockdown the MALAT1 protected MPC-5 cells from pyroptosis and OS induced by HG. However, overexpressing miR-200c in control-group cells increased pyroptosis and upregulated the OS level with HG culture medium. Further, atorvastatin protected MPC-5 cells from cell pyroptosis and downregulated the level of renal OS via attenuating the expression of MALAT1 and miR-200c. Conclusion Atorvastatin protects podocyte cells via MALAT1/miR-200c/NRF2 signal pathway from pyroptosis and OS induced by HG.

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Section: Discussionmentioning

confidence: 99%

“…MiRNAs are noncoding RNAs that regulate gene expression, for example by inhibiting lncRNAs near target genes in the cell nucleus. 55 The mechanisms of miRNA regulation of target genes need further investigation. MiR-200c, a member of miR-200 family, is involved in the regulation of OS.…”

Section: Discussionmentioning

confidence: 99%

Atorvastatin Regulates MALAT1/miR-200c/NRF2 Activity to Protect Against Podocyte Pyroptosis Induced by High Glucose

Zuo¹,

Chen²,

He³

et al. 2021

DMSO

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“…In the previous research, Ma et al and Liu et al found that miRNAs and lncRNA were involved in gonadal development [47,48]. The discovery of small RNAs such as miRNAs, snRNAs, and other types had unveiled a slew of powerful regulators of gene expression in recent years [49].…”

Section: Discussionmentioning

confidence: 99%

“…Liu et al found 665 differentially expressed miRNAs, with 519 being up-regulated in testis and 146 being down-regulated in ovary, respectively [48]. A total of 633 miRNAs showed differential expression in the ovaries and testes [47]. However, the molecular mechanism of miRNA regulating gonadal differentiation is currently unclear.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs May Play an Important Role in Sexual Reversal Process of Chinese Soft-Shelled Turtle, Pelodiscus sinensis

Zhou

Sha

Chen

et al. 2021

Genes

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The Chinese soft-shelled (Pelodiscus sinensis) turtle exhibits obvious sex dimorphism, which leads to the higher economic and nutritional value of male individuals. Exogenous hormones can cause the transformation from male to female phenotype during gonadal differentiation. However, the molecular mechanism related to the sexual reversal process is unclear. In this study, we compared the difference between the small RNAs of male, female, and pseudo-female turtles by small RNA-seq to understand the sexual reversal process of Chinese soft-shelled turtles. A certain dose of estrogen can cause the transformation of Chinese soft-shelled turtles from male to female, which are called pseudo-female individuals. The result of small RNA-seq has revealed that the characteristics of pseudo-females are very similar to females, but are strikingly different from males. The number of the microRNAs (miRNAs) of male individuals was significantly less than the number of female individuals or pseudo-female individuals, while the expression level of miRNAs of male individuals were significantly higher than the other two types. Furthermore, we found 533 differentially expressed miRNAs, including 173 up-regulated miRNAs and 360 down-regulated miRNAs, in the process of transformation from male to female phenotype. Cluster analysis of the total 602 differential miRNAs among females, males, and pseudo-females showed that miRNAs played a crucial role during the sexual differentiation. Among these differential miRNAs, we found 12 miRNAs related to gonadal development and verified their expression by qPCR. The TR-qPCR results confirmed the differential expression of 6 of the 12 miRNAs: miR-26a-5p, miR-212-5p, miR-202-5p, miR-301a, miR-181b-3p and miR-96-5p were involved in sexual reversal process, which was consistent with the results of omics. Using these six miRNAs and some of their target genes, we constructed a network diagram related to gonadal development. We suggest that these miRNAs may play an important role in the process of effective sex reversal, which would contribute to the breeding of all male strains of Chinese soft-shelled turtles.

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“…Nuclear miRNAs may recognize and bind target sequences in gene promoters and other DNA regulatory elements in a sequence-specific manner, and then recruit proteins or complexes responsible for epigenetic modification, which results in chromatin remodeling, resulting in transcriptional activation or gene silencing. Alternatively, nuclear miRNAs inhibit lncRNAs near target genes, thereby regulating gene expression (40). At present, the reasons, mechanisms, and functions of miRNA accumulation in the nucleus remain unclear and require further investigation.…”

Section: Mirna Functionmentioning

confidence: 99%

The Role of Non-coding RNAs in Diabetic Nephropathy-Related Oxidative Stress

Kuang

Zuo

et al. 2021

Front. Med.

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Diabetic nephropathy (DN) is one of the main complications of diabetes and the main cause of diabetic end-stage renal disease, which is often fatal. DN is usually characterized by progressive renal interstitial fibrosis, which is closely related to the excessive accumulation of extracellular matrix and oxidative stress. Non-coding RNAs (ncRNAs) are RNA molecules expressed in eukaryotic cells that are not translated into proteins. They are widely involved in the regulation of biological processes, such as, chromatin remodeling, transcription, post-transcriptional modification, and signal transduction. Recent studies have shown that ncRNAs play an important role in the occurrence and development of DN and participate in the regulation of oxidative stress in DN. This review clarifies the functions and mechanisms of ncRNAs in DN-related oxidative stress, providing valuable insights into the prevention, early diagnosis, and molecular therapeutic targets of DN.

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Genome-wide identification and comparison of differentially expressed profiles of miRNAs and lncRNAs with associated ceRNA networks in the gonads of Chinese soft-shelled turtle, Pelodiscus sinensis

Cited by 16 publications

References 70 publications

Atorvastatin Regulates MALAT1/miR-200c/NRF2 Activity to Protect Against Podocyte Pyroptosis Induced by High Glucose

Atorvastatin Regulates MALAT1/miR-200c/NRF2 Activity to Protect Against Podocyte Pyroptosis Induced by High Glucose

MicroRNAs May Play an Important Role in Sexual Reversal Process of Chinese Soft-Shelled Turtle, Pelodiscus sinensis

The Role of Non-coding RNAs in Diabetic Nephropathy-Related Oxidative Stress

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