Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas comosus (L.) Merr.) Reveal the Role of AcABCG38 in Pollen Development

Abstract

Pineapple (Ananas comosus L.) cultivation commonly relies on asexual reproduction which is easily impeded by many factors in agriculture production. Sexual reproduction might be a novel approach to improve the pineapple planting. However, genes controlling pineapple sexual reproduction are still remain elusive. In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological processes. Whereas, till today the ABC gene family has not been identified in pineapple. Here 100 ABC genes were identified in the pineapple genome and grouped into eight subfamilies (5 ABCAs, 20 ABCBs, 16 ABCCs, 2 ABCDs, one ABCEs, 5 ABCFs, 42 ABCGs and 9 ABCIs). Gene expression profiling revealed the dynamic expression pattern of ABC gene family in various tissues and different developmental stages. AcABCA5, AcABCB6, AcABCC4, AcABCC7, AcABCC9, AcABCG26, AcABCG38 and AcABCG42 exhibited preferential expression in ovule and stamen. Over-expression of AcABCG38 in the Arabidopsis double mutant abcg1-2abcg16-2 partially restored its pollen abortion defects, indicating that AcABCG38 plays important roles in pollen development. Our study on ABC gene family in pineapple provides useful information for developing sexual pineapple plantation which could be utilized to improve pineapple agricultural production.

Genome-wide analysis of ATP binding cassette (ABC) transporters in tomato

“…This approach provides essential information, such as evolutionary history, diversity and relationship among genes and proteins, which serves as useful fundamental resources for further investigations. Genome-wide analyses of ABC transporters in Arabidopsis [ 15 ], rice [ 16 ], maize [ 17 ], Lotus japonicus [ 18 ], grape [ 19 ], pineapple [ 20 ], and Hevea brasiliensis [ 4 ] have already been performed. Whereas little is known about ABC transporters in Solanaceae, including tomato.…”

Understanding the Composition, Biosynthesis, Accumulation and Transport of Flavonoids in Crops for the Promotion of Crops as Healthy Sources of Flavonoids for Human Consumption

“…Based on their characteristic protein structures, ABC transporters have been identified in various plant species. Using genome-wide analyses, 91, 160, 261, 154, 100, 129, 120, 135 and 130 putative genes encoding ABC transporters were identified in the genomes of L. japonicus [ 113 ], M. truncatula [ 114 ], G. max [ 115 ], Solanum lycopersicum [ 116 ], Ananas comosus [ 117 ], Oryza sativa [ 118 ], A. thaliana [ 119 ], V. vinifera [ 120 ] and Zea mays [ 121 ], respectively ( Table 2 ). A phylogenetic analysis of the ABC transporters identified from G. max , S. lycopersicum and A. thaliana , the species with available genome information, is shown in Figure 3 .…”

QTL mapping and identification of SNP-haplotypes affecting yield components of Theobroma cacao L.

“…The other T. cacao candidate gene, Thecc1EG016965t1, is a member of ATP-binding cassette transporter family (ABC transporter) that are associated with transportation of diverse metabolites 44 . Furthermore, those proteins transport fatty acids for lipid synthesis during the seed filling of Arabidopsis thaliana 45 and are crucial during pollen development in Ananas comosus 9 .…”

“…The genes that underpin many yield components exert regulatory control over biomass production and accumulation by acting in metabolic pathways that supply storage forms of nitrogen 6 , synthesize and transport carbon reserve compounds 7,8 and lipids 9 required during crop yield formation.…”