Genome-wide identification and functional analysis of the TIFY gene family in the response to multiple stresses in Brassica napus L.

Abstract

Background TIFY is a plant-specific protein family with a diversity of functions in plant development and responses to stress and hormones, which contains JASMONATE ZIM-domain (JAZ), TIFY, PPD and ZML subfamilies. Despite extensive studies of TIFY family in many other species, TIFY has not yet been characterized in Brassica napus. Results In this study, we identified 77, 36 and 39 TIFY family genes in the genome of B. napus, B. rapa and B. oleracea, respectively. Results of the phylogenetic analysis indicated the 170 TIFY proteins from Arabidopsis, B. napus, B. rapa and B. oleracea could be divided into 11 groups: seven JAZ groups, one PPD group, one TIFY group, and two ZIM/ZML groups. The molecular evolutionary analysis showed that TIFY genes were conserved in Brassicaceae species. Gene expression profiling and qRT-PCR revealed that different groups of BnaTIFY members have distinct spatiotemporal expression patterns in normal conditions or following treatment with different abiotic/biotic stresses and hormones. The BnaJAZ subfamily genes were predominantly expressed in roots and up-regulated by NaCl, PEG, freezing, methyl jasmonate (MeJA), salicylic acid (SA) and Sclerotinia sclerotiorum in leaves, suggesting that they have a vital role in hormone signaling to regulate multiple stress tolerance in B. napus. Conclusions The extensive annotation and expression analysis of the BnaTIFY genes contributes to our understanding of the functions of these genes in multiple stress responses and phytohormone crosstalk in B. napus.

Genome-wide identification and expression analysis of the JAZ gene family in turnip

“…Therefore, we analyzed the expression of JAZ family genes in turnips under different stress conditions. Our results showed that most BrrJAZs responded significantly to abiotic stress and/or exogenous phytohormone treatments, which is in agreement with the results obtained in other Brassica crops 30 , 40 – 42 .…”

“…Previous research identified 36, 38, 36, and 36 JAZ genes in B. rapa L. 40 , B. juncea var. tumida 41 , B. napus L. 42 , and B. oleracea var. capitata 30 , respectively.…”

“…The composition of the turnip JAZ gene family members was more similar to that of other dicotyledons. The JAZ protein sequences of the tea plant 43 , Arabidopsis 44 , tomato 45 , and Brassica 30 , 40 – 42 all contain members of the TIFY, JAZ, and PPD subfamilies, and members of these three subfamilies have been identified in the JAZ family protein sequence of turnip. JAZ family genes have numerous members and are likely to perform different functions in response to adversity stress.…”

Genome-Wide Identification of the TIFY Gene Family in Brassiceae and Its Potential Association with Heavy Metal Stress in Rapeseed

“…Similarly, GaJAZ5 was found to improve drought resistance, and GsTIFY10a may play positive roles in plant tolerance to bicarbonate stress [40]. Recently, results showed that the BnaJAZ subfamily of genes could be predominantly induced by different abiotic/biotic stresses and hormones in rapeseed [41], which showed similar patterns in B. rapa [42] and B. oleracea [43]. In this study, our findings suppose that all TIFY family genes had a tight evolutionary and phylogenetic relationship (Figures 1 and 4), and may share similar functions in the same subgroup.…”

Genome-Wide Identification and Expression Analysis of JAZ Family Involved in Hormone and Abiotic Stress in Sweet Potato and Its Two Diploid Relatives

“…The Jas domain could interact with COI1 and transcription factors that were involved in JA signaling pathway [18]. The Arabidopsis, maize, rice, Brassica napus, Camellia sinensis, and soybean genomes encode 13, 26, 15, 52, 13, and 25 JAZs, respectively [13,[19][20][21][22][23]. The JAZ proteins from different plant species could be divided naturally into different numbers of sub-groups based on their evolutionary relationship [20][21][22][23].…”