Genome-wide identification and low-salinity stress analysis of the Hsp70 gene family in swimming crab (Portunus trituberculatus)

Jin, Shihuai; Deng, Zhaochao; Xu, Shengyong; Zhang, Haobo; Han, Zhiqiang

doi:10.1016/j.ijbiomac.2022.03.055

Cited by 18 publications

(11 citation statements)

References 46 publications

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“…In the present study, a total of 16 HSP70 genes were detected in the turbot, which was more than the number previously found in crustaceans ( Portunus trituberculatus ) (9) [ 40 ] but considerably less than the number found in bivalve species, such as the Crassostrea gigas (88) [ 41 ], Mercenaria mercenaria (133) [ 39 ], and Cyclina sinensis (60) [ 39 ], and similar to the number in humans (17) [ 42 ] and in other teleost species (14-20). Although the number of HSP70 genes varies among species, they display high-level evolutionary conservation in humans and teleost species.…”

Section: Discussionmentioning

confidence: 45%

Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)

Zheng

Chen

et al. 2023

IJMS

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Heat shock proteins 70 (HSP70s) are known to play essential roles in organisms’ response mechanisms to various environmental stresses. However, no systematic identification and functional analysis has been conducted for HSP70s in the turbot (Scophthalmus maximus), a commercially important worldwide flatfish. Herein, 16 HSP70 genes unevenly distributed on nine chromosomes were identified in the turbot at the genome-wide level. Analyses of gene structure, motif composition, and phylogenetic relationships provided valuable data on the HSP70s regarding their evolution, classification, and functional diversity. Expression profiles of the HSP70 genes under five different stresses were investigated by examining multiple RNA-seq datasets. Results showed that 10, 6, 8, 10, and 9 HSP70 genes showed significantly up- or downregulated expression after heat-induced, salinity-induced, and Enteromyxum scophthalmi, Vibrio anguillarum, and Megalocytivirus infection-induced stress, respectively. Among them, hsp70 (hspa1a), hspa1b, and hspa5 showed significant responses to each kind of induced stress, and qPCR analyses further validated their involvement in comprehensive anti-stress, indicating their involvement in organisms’ anti-stress mechanisms. These findings not only provide new insights into the biological function of HSP70s in turbot adapting to various environmental stresses, but also contribute to the development of molecular-based selective breeding programs for the production of stress-resistant turbot strains in the aquaculture industry.

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Section: Discussionmentioning

confidence: 45%

Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)

Zheng

Chen

et al. 2023

IJMS

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“…Gene duplication occurs in three main forms including tandem, fragment, and genome duplication, which are the primary drivers of gene family expansion ( Lee et al, 2015 ) and provide the raw materials for the evolution of gene function ( Jin S. et al, 2022 ). The presence of two or more genes within 200 kb on a chromosome means that there are tandem duplicate gene pairs in the region ( Holub, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolution and expression analysis of bone morphogenetic protein (BMP) gene family in chinese soft-shell turtle (Pelodiscus sinensis)

et al. 2023

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Introduction: Bone morphogenetic proteins (BMPs) play a crucial role in bone formation and differentiation. Recent RNA-Seq results suggest that BMPs may be involved in the sex differentiation of P. sinensis, yet more relevant studies about BMPs in P. sinensis are lacking.Methods: Herein, we identified BMP gene family members, analyzed the phylogeny, collinear relationship, scaffold localization, gene structures, protein structures, transcription factors and dimorphic expression by using bioinformatic methods based on genomic and transcriptomic data of P. sinensis. Meanwhile, qRT-PCR was used to verify the RNA-Seq results and initially explore the function of the BMPs in the sex differentiation of P. sinensis.Results: A total of 11 BMP genes were identified, 10 of which were localized to their respective genomic scaffolds. Phylogenetic analysis revealed that BMP genes were divided into eight subfamilies and shared similar motifs (“WII”, “FPL”, “TNHA”, “CCVP”, and “CGC”) and domain (TGF-β superfamily). The results of the sexually dimorphic expression profile and qRT-PCR showed that Bmp2, Bmp3, Bmp15l, Bmp5, Bmp6 and Bmp8a were significantly upregulated in ovaries, while Bmp2lb, Bmp7, Bmp2bl and Bmp10 were remarkable upregulated in testes, suggesting that these genes may play a role in sex differentiation of P. sinensis.Discussion: Collectively, our comprehensive results enrich the basic date for studying the evolution and functions of BMP genes in P. sinensis.

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“…Many types of environmental stresses such as alterations in temperature, pH, salinity; metal and xenobiotics exposure, and infections can upregulate HSP70 expression in crustaceans (Bao et al, 2014; Baringou et al, 2016; Chang, 2005; Chaurasia et al, 2016; Fernandes et al, 2018; Fu et al, 2013; Jin et al, 2022; Liu et al, 2018; Ortega & Vitorino, 2022; Qian et al, 2012). To our knowledge, only a few studies described upregulation of HSP70 1 h after the beginning of acute stress, such as when the shrimp L. vannamei , the mud crab Scylla paramamosain and the lobster Hommarus americanus were exposed to heat (Chang, 2005; Liu et al, 2018; Qian et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

et al. 2023

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Ocypode quadrata, a Ghost crab species found along the western Atlantic coast, is considered a bioindicator of anthropogenic impact on sandy beaches. Ghost Crabbing, a touristic activity in which ghost crabs are chased just for fun, is a potentially threatening activity for this crab. In crustaceans, metabolites such as glucose and lactate, and the gene expression of crustacean hyperglycemic hormone (CHH) and heat shock proteins (HSPs) increase when the animals are exposed to several types of stress, including alterations in temperature, salinity, or exposure to xenobiotics. This work was developed to identify if being chased by humans would affect these markers of stress in this species of crab. The effects of chasing stress on hemolymph and tissue metabolites and the gene expression levels of CHH and HSP70 were investigated. The levels of lactate in the hemolymph of stressed crabs were six times higher than those of control crabs immediately after chasing and decreased progressively during recovery, indicating an active anaerobic metabolism during the stress. On the contrary, glucose levels in the hemolymph of the stressed crabs increased progressively from 30 to 60 min after chasing, indicating an inverse correlation between glucose and lactate and the conversion of lactate to glucose by gluconeogenesis. In stressed crabs, the levels of triglycerides in the hemolymph decreased 30 min after chasing, while the opposite tended to occur in the hepatopancreas, indicating that during recovery, the crabs use triglycerides as energy source to sustain aerobic metabolism. Finally, this study demonstrates that ghost crabs are stressed by minimum human contact and that “ghost crabbing” must not be encouraged as a tourist activity.

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Genome-wide identification and low-salinity stress analysis of the Hsp70 gene family in swimming crab (Portunus trituberculatus)

Cited by 18 publications

References 46 publications

Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)

Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)

Genome-wide identification, evolution and expression analysis of bone morphogenetic protein (BMP) gene family in chinese soft-shell turtle (Pelodiscus sinensis)

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

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