Genome-wide identification of 14-3-3 gene family and characterization of their expression in developmental stages of Solanum tuberosum under multiple biotic and abiotic stress conditions

Hajibarat, Zahra; Saïdi, A.

doi:10.1007/s10142-022-00895-z

Cited by 7 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, we found that the distribution of RF2 genes on each chromosome was uneven, but we found that most of the number and location of genes on each chromosome of the two tetraploid cotton species were one to one, this phenomena was also observed for other transcription factors in other plant species [43][44][45][46][47][48]. This may be due to the fact that allotetraploids share the common ancestors A0 (the possible extinct common ancestor of the existing A genome) and D5 (G. raimondii) [49].…”

Section: Discussionsupporting

confidence: 59%

Genome-Wide Identification and Functional Analysis of RF2 Gene Family and the Critical Role of GhRF2-32 in Response to Drought Stress in Cotton

Zhao

Wei

et al. 2023

Plants

View full text Add to dashboard Cite

Cotton is an important natural fiber crop. The RF2 gene family is a member of the bZIP transcription factor superfamily, which plays an important role in plant resistance to environmental stresses. In this paper, the RF2 gene family of four cotton species was analyzed genome-wide, and the key gene RF2-32 was cloned for functional verification. A total of 113 RF2 genes were identified in the four cotton species, and the RF2 family was relatively conserved during the evolution of cotton. Chromosome mapping and collinear analysis indicated that fragment replication was the main expansion mode of RF2 gene family during evolution. Cis-element analysis showed that there were many elements related to light response, hormone response and abiotic stress response in the promoters of RF2 genes. The transcriptome and qRT-PCR analysis of RF2 family genes in upland cotton showed that RF2 family genes responded to salt stress and drought stress. GhRF2-32 protein was localized in the cell nucleus. Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance. This study revealed the evolutionary and functional diversity of the RF2 gene family, which laid a foundation for the further study of stress-resistant genes in cotton.

show abstract

Section: Discussionsupporting

confidence: 59%

Genome-Wide Identification and Functional Analysis of RF2 Gene Family and the Critical Role of GhRF2-32 in Response to Drought Stress in Cotton

Zhao

Wei

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Moreover, IbGLR7, IbGLR17, and IbGLR20 were determined to interact with CNGC18, which was reported to regulate germination of pollen grains and the growth of pollen tubes ( Chang et al., 2007 ; Cao et al., 2016 ; Gu et al., 2017 ). IbGLR17, IbGLR20, and IbGLR25 were determined to interact with GF14, which participates in transport, growth, metabolism, and the drought stress reactions ( Li et al., 2016 ; Hajibarat et al., 2022 ). IbGLR2, IbGLR7, and IbGLR17 were determined to interact with TPK1, which participates in potassium transportation, fruit ripening, and quality formation ( Lu, 2011 ; Latza et al., 2013 ; Wang et al., 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification and expression analysis of the glutamate receptor gene family in sweet potato and its two diploid relatives

Hu,

Dai,

Huang

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Plant glutamate receptor (GLR) homologs are crucial calcium channels that play an important role in plant development, signal transduction, and response to biotic and abiotic stresses. However, the GLR gene family has not yet been thoroughly and systematically studied in sweet potato. In this study, a total of 37 GLR genes were identified in the cultivated hexaploid sweet potato (Ipomoea batatas), and 32 GLR genes were discovered in each of the two diploid relatives (Ipomoea trifida and Ipomoea triloba) for the first time. Based on their evolutionary relationships to those of Arabidopsis, these GLRs were split into five subgroups. We then conducted comprehensive analysis to explore their physiological properties, protein interaction networks, promoter cis-elements, chromosomal placement, gene structure, and expression patterns. The results indicate that the homologous GLRs of the cultivated hexaploid sweet potato and its two relatives are different. These variations are reflected in their functions related to plant growth, hormonal crosstalk, development of tuberous roots, resistance to root rot, and responses to abiotic stress factors, all of which are governed by specific individual GLR genes. This study offers a comprehensive analysis of GLR genes in sweet potato and its two diploid relatives. It also provides a theoretical basis for future research into their regulatory mechanisms, significantly influencing the field of molecular breeding in sweet potatoes.

show abstract

“…Plant 14-3-3 proteins have been found to be associated with plant stress response ( Hajibarat et al., 2022 ; Huang et al., 2022 ) and involved in a network of interactions that finely regulate signaling and homeostasis of multiple hormones such as abscisic acid (ABA) ( Schoonheim et al., 2009 ), gibberellins (GAs) ( Ito et al., 2014 ), ethylene ( Catalá et al., 2014 ), and auxin ( Takahashi et al., 2012 ; Camoni et al., 2018 ). In our previous studies, stress treatments such as drought, salt, high and low temperatures, and osmotic testing were applied to AhGRFi -overexpressing Arabidopsis lines, but we did not find significant phenotypic differences (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…The growth and genetic potential of a plant are affected by a variety of environmental factors such as light, temperature, humidity, moisture, lodging, wind velocity, and the internal plant atmosphere, which includes plant hormones, signaling molecules, chlorophyll content, and stomatal movement ( Fulgosi et al., 2002 ). There are small molecular proteins called 14-3-3 proteins in plant cells that play an important role in cell signal transduction in response to biotic and abiotic stresses and regulation of plant growth and development ( Grienenberger and Fletcher, 2015 ; Hajibarat et al., 2022 ; Huang et al., 2022 ). In addition, it also participates in material transport and metabolism during various metabolic processes and plant hormone signaling.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of peanut (Arachis hypogaea L.) AhGRFi gene enhanced root growth inhibition under exogenous NAA treatment in Arabidopsis thaliana

et al. 2023

View full text Add to dashboard Cite

The 14-3-3 protein is a kind of evolutionary ubiquitous protein family highly conserved in eukaryotes. Initially, 14-3-3 proteins were reported in mammalian nervous tissues, but in the last decade, their role in various metabolic pathways in plants established the importance of 14-3-3 proteins. In the present study, a total of 22 14-3-3 genes, also called general regulatory factors (GRF), were identified in the peanut (Arachis hypogaea) genome, out of which 12 belonged to the ε group, whereas 10 of them belonged to the non- ε-group. Tissue-specific expression of identified 14-3-3 genes were studied using transcriptome analysis. The peanut AhGRFi gene was cloned and transformed into Arabidopsis thaliana. The investigation of subcellular localization indicated that AhGRFi is localized in the cytoplasm. Overexpression of the AhGRFi gene in transgenic Arabidopsis showed that under exogenous 1-naphthaleneacetic acid (NAA) treatment, root growth inhibition in transgenic plants was enhanced. Further analysis indicated that the expression of auxin-responsive genes IAA3, IAA7, IAA17, and SAUR-AC1 was upregulated and GH3.2 and GH3.3 were downregulated in transgenic plants, but the expression of GH3.2, GH3.3, and SAUR-AC1 showed opposite trends of change under NAA treatment. These results suggest that AhGRFi may be involved in auxin signaling during seedling root development. An in-depth study of the molecular mechanism of this process remains to be further explored.

show abstract

Genome-wide identification of 14-3-3 gene family and characterization of their expression in developmental stages of Solanum tuberosum under multiple biotic and abiotic stress conditions

Cited by 7 publications

References 32 publications

Genome-Wide Identification and Functional Analysis of RF2 Gene Family and the Critical Role of GhRF2-32 in Response to Drought Stress in Cotton

Genome-Wide Identification and Functional Analysis of RF2 Gene Family and the Critical Role of GhRF2-32 in Response to Drought Stress in Cotton

Genome-wide identification and expression analysis of the glutamate receptor gene family in sweet potato and its two diploid relatives

Overexpression of peanut (Arachis hypogaea L.) AhGRFi gene enhanced root growth inhibition under exogenous NAA treatment in Arabidopsis thaliana

Contact Info

Product

Resources

About