Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in Camellia sinensis (L.)

Han, Jingxue; Wang, Xiaojing; Niu, Suzhen

doi:10.3390/plants12061302

Cited by 5 publications

(2 citation statements)

References 75 publications

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“…By using RT-qPCR confirmation, all these seven genes were significantly upregulated under darkness treatment (Figure 8B) in the oss40-14 mutant relative to WT. In addition, three ROS related TAGs such as ROS-producing 2OG-Fe oxygenase gene (Han et al, 2023) and GLUTATHIONE S-TRANSFERASE 46 gene (Sharma et al, 2014) both were downregulated by 1.9 times, and 2-OXOGLUTARATE-DEPENDENT DIOXYGENASE (Hu et al, 2019;Wang et al, 2022) displayed a substantial 4.6-fold decrease in the oss40-14 mutant compared to the wild type.…”

Section: Oss40-14 Related To Nutrient Recycling and Phosphorylation A...mentioning

confidence: 97%

Involvement of OsS40-14 in ROS and plastid organization related regulatory networks of dark-induced leaf senescence in rice

Habiba,

Fan,

Hong

et al. 2024

Preprint

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Dark-induced senescence triggers significant metabolic changes that recycle resources and ensure plant survival. In this study, we identified a transcription factor OsS40-14 in rice, which can form homo-oligomers. Theoss40-14knockout mutants exhibited stay-green phenotype of primary leaf and flag leaf during dark-induced condition, with substantial retention of chlorophylls and photosynthetic capacity as well as remarkably reduced reactive oxygen species (ROS), whileOsS40-14overexpressing transgenic lines (oeOsS40-14) showed an accelerated senescence phenotype under dark-induced leaf senescence conditions. Transcriptome analysis revealed that when the detached leaves ofoss40-14and WT were treated in darkness condition for 72 hours, 1585 DEGs (|Log2FC| ≥1, P value<0.05) were reprogrammed inoss40-14relative to WT. CUT&Tag-seq analysis in protoplast transient expression of OsS40-14 system showed that OsS40-14 was 40.95% enriched in the transcription start site (TSS) of the genome. Sequence clustering analysis showed that OsS40-14 protein was mainly enriched and bound to TACCCACAAGACAC conserved elements. The seed region “ACCCA” of OsS40 proteins was identified by single nucleotide mutagenesis EMSA. The integrative analysis of transcriptome and CUT&Tag-seq datasets showed 153 OsS40-14-targeted DEGs, they mainly enriched in plastid organization and photosynthesis process at dark-induced condition inoss40-14relative to WT. Among them, eleven candidate targets of OsS40-14 such as Glucose 6-phosphate/phosphate translocator, Na+/H+ antiporter, Catalase, Chitinase 2, Phosphate transporter 19, OsWAK32, and OsRLCK319 were directly targeted and upregulated confirmed by ChIP-PCR and RT-qPCR. It demonstrates a novel model of OsS40-14 mediating macromolecule metabolism and nutrient recycling controls the plastid organization during dark-induced leaf senescence.Significant statementInvolvement of OsS40-14 in macromolecule catabolism, nutrient recycling, and ROS homeostasis revealed a plastid organization defection of dark-induced senescence in rice

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Section: Oss40-14 Related To Nutrient Recycling and Phosphorylation A...mentioning

confidence: 97%

Involvement of OsS40-14 in ROS and plastid organization related regulatory networks of dark-induced leaf senescence in rice

Habiba,

Fan,

Hong

et al. 2024

Preprint

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“…Fe is involved in the biosynthesis of heme molecules [79], and its deficiency leads to physiological hypoxia [82]. Mugineic acid (MA) is involved in Fe translocation in plant tissues as an Fe chelator, and its biosynthesis requires a precursor, nicotianamine (NA, formed from S-adenosyl methionine via nicotianamine synthase (NAS)) in graminaceous plants [83] and 2 ′ -deoxymugineic-acid 2 ′ -dioxygenases [84]. Our results show that all genes encoding PGBs, NAS, 2 ′ -deoxymugineic-acid 2 ′ -dioxygenases, and Fe 2+ transport proteins in the roots were significantly downregulated under NH 4 + treatment and were upregulated after HCO 3 − application (Table 1).…”

Section: O2 Uptake Transport and Consumption May Be Associated With C...mentioning

confidence: 99%

Bicarbonate-Dependent Detoxification by Mitigating Ammonium-Induced Hypoxic Stress in Triticum aestivum Root

Liu,

Zhang,

Tang

et al. 2024

Biology

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Ammonium (NH4+) toxicity is ubiquitous in plants. To investigate the underlying mechanisms of this toxicity and bicarbonate (HCO3−)-dependent alleviation, wheat plants were hydroponically cultivated in half-strength Hoagland nutrient solution containing 7.5 mM NO3− (CK), 7.5 mM NH4+ (SA), or 7.5 mM NH4+ + 3 mM HCO3− (AC). Transcriptomic analysis revealed that compared to CK, SA treatment at 48 h significantly upregulated the expression of genes encoding fermentation enzymes (pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH), and lactate dehydrogenase (LDH)) and oxygen consumption enzymes (respiratory burst oxidase homologs, dioxygenases, and alternative oxidases), downregulated the expression of genes encoding oxygen transporters (PIP-type aquaporins, non-symbiotic hemoglobins), and those involved in energy metabolism, including tricarboxylic acid (TCA) cycle enzymes and ATP synthases, but upregulated the glycolytic enzymes in the roots and downregulated the expression of genes involved in the cell cycle and elongation. The physiological assay showed that SA treatment significantly increased PDC, ADH, and LDH activity by 36.69%, 43.66%, and 61.60%, respectively; root ethanol concentration by 62.95%; and lactate efflux by 23.20%, and significantly decreased the concentrations of pyruvate and most TCA cycle intermediates, the complex V activity, ATP content, and ATP/ADP ratio. As a consequence, SA significantly inhibited root growth. AC treatment reversed the changes caused by SA and alleviated the inhibition of root growth. In conclusion, NH4+ treatment alone may cause hypoxic stress in the roots, inhibit energy generation, suppress cell division and elongation, and ultimately inhibit root growth, and adding HCO3− remarkably alleviates the NH4+-induced inhibitory effects on root growth largely by attenuating the hypoxic stress.

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Phenanthrene metabolism in Panicum miliaceum: anatomical adaptations, degradation pathway, and computational analysis of a dioxygenase enzyme

Tarigholizadeh,

Motafakkerazad,

Mohajel Kazemi

et al. 2024

Environ Sci Pollut Res

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Genome-Wide Identification of 2-Oxoglutarate and Fe (II)-Dependent Dioxygenase (2ODD-C) Family Genes and Expression Profiles under Different Abiotic Stresses in Camellia sinensis (L.)

Cited by 5 publications

References 75 publications

Involvement of OsS40-14 in ROS and plastid organization related regulatory networks of dark-induced leaf senescence in rice

Involvement of OsS40-14 in ROS and plastid organization related regulatory networks of dark-induced leaf senescence in rice

Bicarbonate-Dependent Detoxification by Mitigating Ammonium-Induced Hypoxic Stress in Triticum aestivum Root

Phenanthrene metabolism in Panicum miliaceum: anatomical adaptations, degradation pathway, and computational analysis of a dioxygenase enzyme

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