Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Li, Yajun; Fei, Xiaowen; Dai, Hao‐Fu; Li, Jiangyue; Zhu, Wei; Deng, Xiaodong

doi:10.3389/fpls.2019.01147

Cited by 11 publications

(3 citation statements)

References 63 publications

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“…In fact, the genome of the unicellular green microalga C. reinhardtii encodes 17 MAPKs, 2 MAPKKs, and 108 MAPKKKs [36], which is in accord with findings in the multicellular U. compressa, except for the larger number of MAPKKKs found in C. reinhardtii. In addition, 15 genes encoding CDPKs were identified in the genome of C. reinhardtii [37], which is in accord with those present in the genome of U. compressa.…”

Section: U Compressa Encodes Signal Transduction Protein Kinasessupporting

confidence: 61%

The Genome of the Marine Alga Ulva compressa (Chlorophyta) Reveals Protein-Coding Genes with Similarity to Plants and Green Microalgae, but Also to Animal, Bacterial, and Fungal Genes

Osório

Tapia-Reyes

Espinoza

et al. 2022

IJMS

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The genome of the marine alga Ulva compressa was assembled using long and short reads. The genome assembly was 80.8 Mb in size and encoded 19,207 protein-coding genes. Several genes encoding antioxidant enzymes and a few genes encoding enzymes that synthesize ascorbate and glutathione were identified, showing similarity to plant and bacterial enzymes. Additionally, several genes encoding signal transduction protein kinases, such as MAPKs, CDPKS, CBLPKs, and CaMKs, were also detected, showing similarity to plants, green microalgae, and bacterial proteins. Regulatory transcription factors, such as ethylene- and ABA-responsive factors, MYB, WRKY, and HSTF, were also present and showed similarity to plant and green microalgae transcription factors. Genes encoding enzymes that synthesize ACC and ABA-aldehyde were also identified, but oxidases that synthesize ethylene and ABA, as well as enzymes that synthesize other plant hormones, were absent. Interestingly, genes involved in plant cell wall synthesis and proteins related to animal extracellular matrix were also detected. Genes encoding cyclins and CDKs were also found, and CDKs showed similarity to animal and fungal CDKs. Few genes encoding voltage-dependent calcium channels and ionotropic glutamate receptors were identified as showing similarity to animal channels. Genes encoding Transient Receptor Potential (TRP) channels were not identified, even though TRPs have been experimentally detected, indicating that the genome is not yet complete. Thus, protein-coding genes present in the genome of U. compressa showed similarity to plant and green microalgae, but also to animal, bacterial, and fungal genes.

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Section: U Compressa Encodes Signal Transduction Protein Kinasessupporting

confidence: 61%

The Genome of the Marine Alga Ulva compressa (Chlorophyta) Reveals Protein-Coding Genes with Similarity to Plants and Green Microalgae, but Also to Animal, Bacterial, and Fungal Genes

Osório

Tapia-Reyes

Espinoza

et al. 2022

IJMS

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“…Calcium-dependent protein kinases are the key proteins of plant signal transduction, which transmit important second messenger Ca 2+ through phosphorylation of various substrates ( Li et al, 2019a ). CDPKs play an important role in various physiological reactions, such as stem and root development, pollen tube growth, stomatal movement, hormone signal transduction, transcriptional reprogramming, and stress resistance, etc.…”

Section: Discussionmentioning

confidence: 99%

Calcium-Dependent Protein Kinase GhCDPK28 Was Dentified and Involved in Verticillium Wilt Resistance in Cotton

Zhang

Zhou

et al. 2021

Front. Plant Sci.

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Verticillium dahliae is a soil-borne fungus that causes vascular wilt through the roots of plants. Verticillium wilt caused by V. dahliae is one of the main diseases in cotton producing areas of the world, resulting in huge economic losses. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. Calcium-dependent protein kinases (CDPKs) play a pivotal role in plant innate immunity, including regulation of oxidative burst, gene expression as well as hormone signal transduction. However, the function of cotton CDPKs in response to V. dahliae stress remains unexplored. In this study, 96, 44 and 57 CDPKs were identified from Gossypium hirsutum, Gossypium raimondii and Gossypium arboretum, respectively. Phylogenetic analysis showed that these CDPKs could be divided into four branches. All GhCDPKs of the same clade are generally similar in gene structure and conserved domain arrangement. Cis-acting elements related to hormones, stress response, cell cycle and development were predicted in the promoter region. The expression of GhCDPKs could be regulated by various stresses. Gh_D11G188500.1 and Gh_A11G186100.1 was up-regulated under Vd0738 and Vd991 stress. Further phosphoproteomics analysis showed that Gh_A11G186100.1 (named as GhCDPK28-6) was phosphorylated under the stress of V. dahliae. Knockdown of GhCDPK28-6 expression, the content of reactive oxygen species was increased, a series of defense responses were enhanced, and the sensitivity of cotton to V. dahliae was reduced. Moreover, overexpression of GhCDPK28-6 in Arabidopsis thaliana weakened the resistance of plants to this pathogen. Subcellular localization revealed that GhCDPK28-6 was localized in the cell membrane. We also found that GhPBL9 and GhRPL12C may interact with GhCDPK28-6. These results indicate that GhCDPK28-6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties.

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“…Moreover, the CDPK family of Ca 2+ sensor kinases, that play a central role in response to biotic and abiotic stimuli in land plants, have recently been found to be encoded by 15 different genes in C. reinhardtii , and to also be involved in nitrogen deficiency-induced oil accumulation (Y. Li et al , 2019 ). Recently, 13 novel CBL-like proteins have also been identified through a genome-wide analysis in C. reinhardtii , displaying characteristic EF-hand Ca 2+ -binding domains like those present in Arabidopsis CBLs ( Kumar et al , 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Chlamydomonas reinhardtii cellular compartments and their contribution to intracellular calcium signalling

Pivato

Ballottari

2021

Journal of Experimental Botany

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Calcium (Ca 2+)-dependent signalling plays a well-characterized role in the response to different environmental stimuli, both in plant and animal cells. In the model organism for green algae, Chlamydomonas reinhardtii, Ca 2+ signals were reported having a crucial role in different physiological processes, like stress responses, photosynthesis, and flagella functions. Recent reports identified the underlying components of the Ca 2+ signalling machinery at the level of specific subcellular compartments and reported in vivo imaging of cytosolic Ca 2+ concentration in response to environmental stimuli. The characterization of these Ca 2+-related mechanisms and proteins in C. reinhardtii is providing knowledge on how microalgae can perceive and respond to the environmental stimuli, but also on how this Ca 2+ signalling machinery has evolved. Here, we review the current knowledge on the cellular mechanisms underlying the generation, shaping, and decoding of Ca 2+ signals in C. reinhardtii, providing an overview of the known and possible molecular players involved in the Ca 2+ signalling of its different subcellular compartments. The advanced toolkits recently developed to measure time-resolved Ca 2+ signalling in living C. reinhardtii cells are also discussed, suggesting how they can improve the study of the role of Ca 2+ signals in microalgae cellular response to the environmental stimuli.

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Genome-Wide Identification of Calcium-Dependent Protein Kinases in Chlamydomonas reinhardtii and Functional Analyses in Nitrogen Deficiency-Induced Oil Accumulation

Cited by 11 publications

References 63 publications

The Genome of the Marine Alga Ulva compressa (Chlorophyta) Reveals Protein-Coding Genes with Similarity to Plants and Green Microalgae, but Also to Animal, Bacterial, and Fungal Genes

The Genome of the Marine Alga Ulva compressa (Chlorophyta) Reveals Protein-Coding Genes with Similarity to Plants and Green Microalgae, but Also to Animal, Bacterial, and Fungal Genes

Calcium-Dependent Protein Kinase GhCDPK28 Was Dentified and Involved in Verticillium Wilt Resistance in Cotton

Chlamydomonas reinhardtii cellular compartments and their contribution to intracellular calcium signalling

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