Genome-wide identification of glutathione S-transferase gene family members in tea plant (Camellia sinensis) and their response to environmental stress

Cao, Qinghai; Lv, Wuyun; Jiang, Hong; Chen, Xueling; Wang, Xinchao

doi:10.1016/j.ijbiomac.2022.03.109

Cited by 34 publications

(23 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, for further evolutionary studies, the divergence time of Rp gene pairs was calculated in the two varieties ( Supplementary Datasheet S4 ), and the divergence time started 84.90 Mya to 12.22 Mya between S. litura and B. mori . Additionally, Ka/Ks (the ratio of nonsynonymous substitution to synonymous substitution) value < 1 indicates that a gene pair has experienced negative selection, whereas Ka/Ks > 1 indicates positive selection, and Ka/Ks = 1 indicates neutral selection ( Cao et al, 2022 ). Our results showed that the Ka/Ks ratio varied from 0 to 0.0956, suggesting the evolution of Rp genes between S. litura and B. mori has experienced purify selection.…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 4 and Supplementary Datasheet S2 , we found that SlRp genes had different amounts of introns and exons, intimating these genes exhibited a high degree of complexity in structure and biological functions ( Song et al, 2015 ; Yang et al, 2022 ). Based on the multiple sequence alignments, we found that the Ka/Ks ratios of all SlRp genes were smaller than 1, indicating that most SlRp genes have experienced extensive purifying selection ( Supplementary Datasheet S4 ) ( Cao et al, 2022 ). In addition, when it comes to the physicochemical properties of proteins, we found that positively charged residues (including Arg and Lys) were enriched in most Sl Rps, which might due to that positively charged amino acids play significant roles in electrostatic protein-RNA interactions within the ribosomal complex ( Law et al, 2006 ; Takakura et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Identification and Stage-Specific Expression Profile Analysis Reveal the Function of Ribosomal Proteins for Oogenesis of Spodoptera litura

Sun

Liu

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Ribosomal proteins (Rps) are indispensable in ribosome biogenesis and protein synthesis, which tightly correlate with cell growth and proliferation in different physiological processes across species. Up to now, genes coding for Rps have been identified and studied in many species, however, their information still remains elusive in many insect species, especially in Spodoptera litura. In this study, 81 Rp genes were identified from S. litura genome and were mapped to their positions on the chromosomes. In addition, their physical and chemical properties, gene structure, phylogenetic relationships, targeted microRNAs were also analyzed. Gene ontology analysis disclosed that Rp genes were closely associated with processes related to ribosome biosynthesis, proteins translation processing, molecular binding activities. The quantitative real-time PCR (qRT-PCR) revealed expression profiles of Rp genes varied in different stages of oogenesis, and found that most Rp genes accumulated in previtellogenesis stage. This study described the comprehensive genome-wide analysis of Rp gene family in agricultural pests, which provided foundation for further characterizing the roles of Rps in oogenesis of insects, and some Rp genes may further serve as targets for innovative pest control.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Stage-Specific Expression Profile Analysis Reveal the Function of Ribosomal Proteins for Oogenesis of Spodoptera litura

Sun

Liu

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…Glutathione S-transferase plays a crucial role in intracellular redox homeostasis and response to oxidative stress. It generally catalyzes the conjugation of glutathione and functions as a carrier of secondary metabolites to appropriate cellular localization [ 36 , 37 ]. This binding increases the hydrophobicity of the protein, which can be easily transported across the cell membrane and released externally.…”

Section: Resultsmentioning

confidence: 99%

Storage Drives Alterations of Proteomic and Protein Structural Properties in Rice (Oryza sativa L.)

Wang

Zhang

Liu

et al. 2022

Foods

View full text Add to dashboard Cite

Rice quality changes during storage. However, few studies have reported the difference in protein structure between the indica and japonica varieties of rice during storage. The current research characterized the structural properties of the rice protein, and further investigated the proteomic profiles of Jianzhen 2 (indica rice) and Nanjing 9108 (japonica rice) during storage using the TMT labeling method. A significant reduction in free sulfhydryl content and an increase in disulfide bonds content and surface hydrophobicity were observed in both varieties after storage. The results of FTIR indicated that the changes in the protein’s secondary structure of Nanjing 9108 (japonica rice) were more significant than in Jianzhen 2 (indica rice). A total of 4039 proteins in Nanjing 9108 and 4301 proteins in Jianzhen 2 were identified by TMT-labeled proteomics analysis in this study. Significantly, changes were detected in 831 proteins in Nanjing 9108, while only in 60 proteins in Jianzhen 2. Protein processing in endoplasmic reticulum, starch, and sucrose metabolism were both accelerated in both varieties, while oxidative phosphorylation in mitochondria, glycolysis, fatty acid metabolism, and glutathione metabolism were enhanced in Nanjing 9108 (japonica rice). This study provides insight into the proteomic changes and protein structure in rice induced by storage.

show abstract

“…They are involved in multiple cellular pathways like secondary metabolism, anthocyanin accumulation (Shao et al 2021), signal transduction pathways (Nianiou-Obeidat et al 2017), tetrapyrrole metabolism, and retrograde signaling (Sylvestre Gonon et al 2020), apoptosis, plant growth and development (Basantani et al 2007), and against various biotic and abiotic stresses like oxidative stress tolerance, drought stress, herbicides, antibiotic resistance, etc. (Cao et al 2022;Basantani et al 2011), glucosinolate biosynthesis and metabolism (Liu et al 2014). These glutathione conjugation reactions are important in herbicide selectivity (Chronopoulou et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Based on their localization, GSTs are also known to be cytosolic, microsomal, and mitochondrial (Vaish et al 2020). Currently, genome-wide identi cation and characterization of the GST gene family have been performed in many plant species like melon (Song et al 2020), tea plant (Cao et al 2022), banana (Vaish et al 2022), pumpkin (Kayum et al 2018), radish (Gao et al 2020), etc. Wheat is the only plant species with more than 330 GST genes (Wang et al 2019b).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of Glutathione S-transferase gene family in Cajanus cajan and their expression profiling under different developmental stages in anatomical tissues

Vaish

Gupta

Jain³

et al. 2022

Preprint

View full text Add to dashboard Cite

Plant glutathione S-transferases (GSTs) are multifunctional conserved protein superfamily that is involved in various biological processes such as growth and development, cellular detoxification, stress biology, and various signaling processes. In the current study, a comprehensive genome-wide identification and characterization of the GST gene family were performed in the agriculturally important legume crop Cajanus cajan. A total of 68 GST genes were identified that belong to eight GST classes based on their conserved domains and motifs. Among 68 CcGST genes, 37 CcGST genes were found on seven Cajanus chromosomes and the remaining CcGST genes were found on the scaffold. Segmental and tandem duplication, both were the driving forces of CcGST gene family expansion. The conserved pattern of exon and intron structure among the different GST classes was observed. The secondary prediction showed the dominance of α- helices. Ser is the highly phosphorylated site in CcGSTs. The subcellular localization prediction of CcGSTs revealed their dominance in the cytoplasm. The physicochemical properties of major CcGST proteins reveal that they are acidic in nature. The expression profiling study revealed the high expression of CcGSTU38, CcGSTU40, CcGSTU44, CcGSTL3, CcGSTL4, CcEF1G1, CcEF1G2, CcDHAR2 and CcGSTF6 in most of the developmental stages in different anatomical tissues. The molecular docking study of highly expressed CcGSTU38 with eight herbicide safeners revealed its highest binding affinity with Fenclorim (-5.44 kcal/mol). This gene could be a potential candidate for future molecular characterization under herbicide stress. The results of the current study endow us with the further functional analysis of Cajanus GSTs in the future.

show abstract

Genome-wide identification of glutathione S-transferase gene family members in tea plant (Camellia sinensis) and their response to environmental stress

Cited by 34 publications

References 57 publications

Genome-Wide Identification and Stage-Specific Expression Profile Analysis Reveal the Function of Ribosomal Proteins for Oogenesis of Spodoptera litura

Genome-Wide Identification and Stage-Specific Expression Profile Analysis Reveal the Function of Ribosomal Proteins for Oogenesis of Spodoptera litura

Storage Drives Alterations of Proteomic and Protein Structural Properties in Rice (Oryza sativa L.)

Genome-wide identification and characterization of Glutathione S-transferase gene family in Cajanus cajan and their expression profiling under different developmental stages in anatomical tissues

Contact Info

Product

Resources

About