Genome-wide identification of Grainy head targets in <i>Drosophila</i> reveals regulatory interactions with the POU-domain transcription factor, Vvl

Yao, Liqun; Wang, Shenqiu; Westholm, Jakub Orzechowski; Dai, Qigen; Matsuda, Ryotaro; Hosono, Chie; Bray, Sarah; Lai, Eric C.; Samakovlis, Christos

doi:10.1242/dev.143297

Cited by 26 publications

(29 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 H), corroborating the notion that Yki is a transcriptional activator of Alas. Interestingly, duox mRNA, which encodes an enzyme that catalyzes the oxidation of two tyrosines to di-tyrosine (Edens et al, 2001) and which is also expressed in developing tracheae (Yao et al, 2017) did not show any significant difference in yki mutants ( Fig. 4 H).…”

Section: Yorkie Is Required For Proper Cross-linking Of Tracheal Apicmentioning

confidence: 96%

Yorkie controls tube length and apical barrier integrity in the developingDrosophilaairways

Papadopoulos

Tomančák

Tsarouhas

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Epithelial organ size and shape depend on cell shape changes, cell-matrix communication and apical membrane growth. The Drosophila embryonic tracheal network is an excellent model to study these processes. Here, we show that the transcriptional co-activator of the Hippo pathway, Yorkie (YAP in vertebrates), plays distinct roles in the developing Drosophila airways.Yorkie exerts a cytoplasmic function by binding Drosophila Twinstar, the orthologue of the vertebrate actin-severing protein Cofilin, to regulate F-actin levels and apical cell membrane size, which are required for proper tracheal tube elongation. Second, Yorkie controls water-tightness of tracheal tubes by transcriptional regulation of the enzyme δ-aminolevulinate synthase (Alas).We conclude that Yorkie has a dual role in tracheal development to ensure proper tracheal growth and functionality. Short SummaryThis work identified an alternative role of the transcriptional co-activator Yorkie (Yki) in controlling water impermeability and tube size of the developing Drosophila airways. Tracheal impermeability is triggered by Ykimediated transcriptional regulation of δ-aminolevulinate synthase, Alas, whereas tube elongation is controlled by binding of Yki to the actin severing factor Twinstar.

show abstract

Section: Yorkie Is Required For Proper Cross-linking Of Tracheal Apicmentioning

confidence: 96%

Yorkie controls tube length and apical barrier integrity in the developingDrosophilaairways

Papadopoulos

Tomančák

Tsarouhas

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…HMGB1/2 [128,129] Grh Die at embryo stage, no homeotic phenotype [130] Enhance Sce [108] Grainy head DNA binding domain (T/C)NAAC(C/T)GGT(T/C)(T/C)TGCGG [131]. Genome-wide consensus [132]. CP2 [133] Z Viable and fertile, no homeotic phenotype [134] Myb/SANT-like DNA binding domain (c/T)GAG(C/T)G [135]; genome-wide consensus [136].…”

Section: Dsp1mentioning

confidence: 99%

Drosophila DNA-Binding Proteins in Polycomb Repression

2018

View full text Add to dashboard Cite

Abstract:The formation of individual gene expression patterns in different cell types is required during differentiation and development of multicellular organisms. Polycomb group (PcG) proteins are key epigenetic regulators responsible for gene repression, and dysregulation of their activities leads to developmental abnormalities and diseases. PcG proteins were first identified in Drosophila, which still remains the most convenient system for studying PcG-dependent repression. In the Drosophila genome, these proteins bind to DNA regions called Polycomb response elements (PREs). A major role in the recruitment of PcG proteins to PREs is played by DNA-binding factors, several of which have been characterized in detail. However, current knowledge is insufficient for comprehensively describing the mechanism of this process. In this review, we summarize and discuss the available data on the role of DNA-binding proteins in PcG recruitment to chromatin.

show abstract

“…The Grh family of transcription factors is essential in defining epithelial cell fate (Boglev et al, 2011;Bray and Kafatos, 1991;Bray et al, 1988;Bray et al, 1989;Hemphälä et al, 2003;Nevil et al, 2017;Rifat et al, 2010;Traylor-Knowles et al, 2010;Uv et al, 1994;Uv et al, 1997;Wang and Samakovlis, 2012;Wilanowski et al, 2002;Yao et al, 2017). Grh proteins are highly conserved, with members found in all metazoans examined to date and with more distant relatives found in fungi (Paré et al, 2012;Traylor-Knowles et al, 2010;Venkatesan et al, 2003;Wang and Samakovlis, 2012;Wilanowski et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Grh proteins are highly conserved, with members found in all metazoans examined to date and with more distant relatives found in fungi (Paré et al, 2012;Traylor-Knowles et al, 2010;Venkatesan et al, 2003;Wang and Samakovlis, 2012;Wilanowski et al, 2002). Extensive work in Drosophila and mammalian cell culture has demonstrated that Grh acts to define an epithelial gene regulatory network during metazoan development (Boglev et al, 2011;Bray and Kafatos, 1991;Chen et al, 2016;Gao et al, 2013;Hemphälä et al, 2003;Nevil et al, 2017;Nishino et al, 2017;Senga et al, 2012;Wang and Samakovlis, 2012;Yao et al, 2017). More recently, Grh has been shown to have pioneerfactor activity, as it is required to maintain chromatin accessibility at enhancers in Drosophila eye imaginal discs and can poise these enhancers for transcriptional activation (Jacobs et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Establishment of chromatin accessibility by the conserved transcription factor Grainy head is developmentally regulated

Nevil

Gibson

Bartolutti

et al. 2019

Preprint

View full text Add to dashboard Cite

The dramatic changes in gene expression required for development necessitate the establishment of cis-regulatory modules defined by regions of accessible chromatin. Pioneer transcription factors have the unique property of binding closed chromatin and facilitating the establishment of these accessible regions. Nonetheless, much of how pioneer transcription factors coordinate changes in chromatin accessibility during development remains unknown. To determine whether pioneer-factor function is intrinsic to the protein or whether pioneering activity is developmentally modulated, we studied the highly conserved, essential transcription factor, Grainy head (Grh). Grh is expressed throughout Drosophila development and functions as a pioneer factor in the larvae. We demonstrated that Grh remains bound to condensed mitotic chromosomes, a property shared with other pioneer factors. By assaying chromatin accessibility in embryos lacking either maternal or zygotic Grh at three stages of development, we discovered that Grh is not required for chromatin accessibility in early embryogenesis, in contrast to its essential functions later in development. Our data reveal that the pioneering activity of Grh is temporally regulated and is likely influenced by additional factors expressed at a given developmental stage. Abdusselamoglu, M. D., Eroglu, E., Burkard, T. R. and Knoblich, J. A. (2019). The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop. Elife 8,.Aleksic, J., Ferrero, E., Fischer, B., Shen, S. and Russell, S. (2013). The role of Dichaete in transcriptional regulation during Drosophila embryonic development. BMC Genomics 14, 861.Attardi, L. D. and Tjian, R. (1993). Drosophila tissue-specific transcription factor NTF-1 contains a novel isoleucine-rich activation motif. Genes Dev. 7, 1341-1353. Bellec, M., Radulescu, O. and Lagha, M. (2018). Remembering the past: Mitotic bookmarking in a developing embryo. Curr. Opin. Syst. Biol. 11, 41-49. Benedyk, M. J., Mullen, J. R. and DiNardo, S. (1994). odd-paired: a zinc finger pair-rule protein required for the timely activation of engrailed and wingless in Drosophila embryos. Genes Dev. 8, 105-117. Blythe, S. A. and Wieschaus, E. F. (2016). Establishment and maintenance of heritable chromatin structure during early Drosophila embryogenesis. Elife 5,. Boglev, Y., Wilanowski, T., Caddy, J., Parekh, V., Auden, A., Darido, C., Hislop, N. R., Cangkrama, M., Ting, S. B. and Jane, S. M. (2011). The unique and cooperative roles of the Grainy head-like transcription factors in epidermal development reflect unexpected target gene specificity. Dev. Biol. 349, 512-522. Bolger, A. M., Lohse, M. and Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114-2120. . A cis-acting element and associated binding factor required for CNS expression of the Drosophila melanogaster dopa decarboxylase gene. EMBO J. 7, 177-188. Bray, S. J., Burke, B., Brown, N. H. and Hirsh, J...

show abstract

Genome-wide identification of Grainy head targets in Drosophila reveals regulatory interactions with the POU-domain transcription factor, Vvl

Cited by 26 publications

References 73 publications

Yorkie controls tube length and apical barrier integrity in the developingDrosophilaairways

Yorkie controls tube length and apical barrier integrity in the developingDrosophilaairways

Drosophila DNA-Binding Proteins in Polycomb Repression

Establishment of chromatin accessibility by the conserved transcription factor Grainy head is developmentally regulated

Contact Info

Product

Resources

About