Genome-wide identification of HSP70/110 genes in sea cucumber Apostichopus japonicus and comparative analysis of their involvement in aestivation

Gao, Lei; Yuan, Zihao; Yu, Simeng; Yang, Yujia; Li, Yunfeng; He, Chongbo

doi:10.1016/j.cbd.2018.07.005

Cited by 12 publications

(16 citation statements)

References 66 publications

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“…Molecular chaperones are crucial to this effort because they provide a “buffer” that helps protect cellular proteins from extreme conditions such as sudden temperature rises, oxidative stress, heavy metal exposure, oxygen deprivation and metabolic disorders ( Craig, 2018 ; Fernandez-Fernandez et al., 2017 ; Nillegoda, Wentink & Bukau, 2018 ; Mogk et al., 1999 ). Hsp70/110s is a molecular chaperone that enhances the response of cells to various stress stimuli such as heat shock ( Gao et al, 2018 ). Recent research has made significant progress in better understanding the mechanisms of action of Hsp70 and its co-partners, providing insights into the structural and functional characteristics of different members of the network.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

Luo

2020

PeerJ

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Heat shock proteins (Hsps) play an important role in many biological processes. However, as a typical cold water fish, the systematic identification of Hsp70/110 gene family of rainbow trout (Oncorhynchus mykiss) has not been reported, and the role of Hsp70/110 gene in the evolution of rainbow trout has not been described systematically. In this study, bioinformatics methods were used to analyze the Hsp70/110 gene family of rainbow trout. A total of 16 hsp70/110 genes were identified and classified into ten subgroups. The 16 Hsp70/110 genes were all distributed on chromosomes 2, 4, 8 and 13. The molecular weight is ranged from 78.93 to 91.39 kD. Gene structure and motif composition are relatively conserved in each subgroup. According to RNA-seq analysis of rainbow trout liver and head kidney, a total of four out of 16 genes were significantly upregulated in liver under heat stress, and a total of seven out of 16 genes were significantly upregulated in head kidney. RT-qPCR was carried out on these gene, and the result were consistent with those of RNA-seq. The significantly regulated expressions of Hsp70/110 genes under heat stress indicats that Hsp70/110 genes are involved in heat stress response in rainbow trout. This systematic analysis provided valuable information about the diverse roles of Hsp70/110 in the evolution of teleost, which will contribute to the functional characterization of Hsp70/110 genes in further research.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

Luo

2020

PeerJ

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“…Within aestivation, body wall became soft and thin. Previous study showed that AjHSP70IVAs were significantly up‐regulated to minimize the negative impact of heat stress on body wall (Gao et al, ). While in this study, TLR signalling pathway genes were all down‐regulated in body wall under each temperature stress.…”

Section: Primers Used For Tlr Signalling Pathway Expression Analysismentioning

confidence: 99%

Expression analysis of genes involved in TLR signalling pathway under temperature stress in sea cucumber Apostichopus japonicus

et al. 2019

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Sea cucumber, Apostichopus japonicus, is an important economic species for its medical and nutritional value. The widely pond-cultured A. japonicus had produced great benefits for aquaculture farmers. Environment investigation showed that its living water temperature had dramatic seasonal variations (Dong, Ji, Meng, Dong, & Sun, 2010). Temperature becomes a key environmental factor affecting the survival, growth and reproduction of sea cucumber (Yang et al., 2005). One of the most representative effects of temperature on A. japonicus is aestivation. When the temperature rises to 25°C, A. japonicus will enter into aestivation depending on the age and body size (Liu et al., 1996). Aestivation is a survival strategy to endure high temperature and induces a series of physiological and morphological changes in sea cucumber (

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“…HSPs were categorized into several families according to their molecular sizes, including HSP110, HSP90, HSP70, HSP60, HSP40, small HSP (SHSP) and the HSP10 subclasses (Heck, Schöler, & de Bittencourt, ; Luo, Ahola, Shu, Xu, & Wang, ). HSP70 and HSP110 were included in a previous study of ours (Gao et al, ). In this research, we focused on SHSP, HSP60/10 and HSP90 due to their similar roles, one of which includes promoting the functional refolding of proteins after urea denaturation (Jakob, Gaestel, Engel, & Buchner, ).…”

Section: Introductionmentioning

confidence: 99%

“…The aquaculture of sea cucumbers has resulted from their overfishing. However, adult sea cucumbers always enter into a stage of estivation in the summer that is triggered by chronic heat stress with water temperatures above approximately 25°C (Dong & Dong, ; Gao et al, ; Yang et al, ). Different features can be found in sea cucumbers in estivation including less activity, weight loss, hypometabolism, immune system modification and so on (Dong & Dong, ; Dong, Dong, Tian, Wang, & Zhang, ; Liu et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…First, there should be another regulatory mode of estivation due to the lack of arid conditions in estivation of sea cucumbers, and this also makes sea cucumbers an excellent model organism to study estivation that is induced by high temperature. Second, juvenile sea cucumbers do not use the estivation strategy under chronic heat stress, making them different from adult individuals (Gao et al, ; Yuan et al, ). This difference provides us with a good comparative studying subject in the research of estivation in the sea cucumber.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide comparative analysis of theSHSP,HSP60/10andHSP90genes reveals differential heat stress responses in estivation of the sea cucumberApostichopus japonicus

Gao

Yuan

et al. 2019

Aquac Res

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Small HSP (SHSP), HSP60/10 and HSP90 are subgroups of heat shock proteins and play important roles in cytoprotective, housekeeping and apoptosis regulatory functions. These proteins are always involved in the response to stressful stimuli, especially heat stress. In this study, 10 SHSPs, two HSP60/10s and three HSP90s were identified and systematically characterized from the transcriptome and genome of the sea cucumber Apostichopus japonicus. According to the results of phylogenetic and syntenic analyses, although the AjSHSPs were less evolved in that they did not occupy the specific domain, there was a mild expansion of AjSHSPs that made the sea cucumber the species that had the most SHSPs among the invertebrate species that were evaluated in this study. The expression of five copies in Class I SHSPs (AjHSP24.3, AjHSP25.5, AjHSP25.6, AjHSP26.1 and AjHSP29.8) tended to be most regulated in estivation in adult individuals, while they exhibited differential patterns in juvenile individuals, suggesting their critical roles in the response to estivation and that different strategies were used in the response to chronic heat shock between adult and juvenile individuals. AjHSP60/10s and AjHSP90s were found to be relatively mildly regulated and were suggested to play additional roles in housekeeping. Together, these findings may provide an early insight into the involvement of SHSPs, HSP60/10s and HSP90s in estivation of marine invertebrate.

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Genome-wide identification of HSP70/110 genes in sea cucumber Apostichopus japonicus and comparative analysis of their involvement in aestivation

Cited by 12 publications

References 66 publications

Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

Expression analysis of genes involved in TLR signalling pathway under temperature stress in sea cucumber Apostichopus japonicus

Genome-wide comparative analysis of theSHSP,HSP60/10andHSP90genes reveals differential heat stress responses in estivation of the sea cucumberApostichopus japonicus

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