Genome-Wide Identification of NAC Transcription Factors and Their Functional Prediction of Abiotic Stress Response in Peanut

Li, Pengxiang; Peng, Zhenying; Xu, Pingli; Tang, Guiying; Ma, Changle; Zhu, Jieqiong; Shan, Lei

doi:10.3389/fgene.2021.630292

Cited by 32 publications

(33 citation statements)

References 71 publications

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“…Among the 20 motifs, motif 2, motif 4, motif 3, and motif 5 had the subdomains A, B, D, and E, respectively, and motifs 1 and 6 contained subdomain C (Figure 2D). These results are consistent with the finding that a connection existed between subfamilies and motifs [37].…”

Section: Gene Structure and Conserved Motif Analyses Of Hunacssupporting

confidence: 93%

HuNAC20 and HuNAC25, Two Novel NAC Genes from Pitaya, Confer Cold Tolerance in Transgenic Arabidopsis

Xie

Liang

et al. 2022

IJMS

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NAC transcription factors are one of the largest families of transcriptional regulators in plants, and members of the gene family play vital roles in regulating plant growth and development processes including biotic/abiotic stress responses. However, little information is available about the NAC family in pitaya. In this study, we conducted a genome-wide analysis and a total of 64 NACs (named HuNAC1-HuNAC64) were identified in pitaya (Hylocereus). These genes were grouped into fifteen subgroups with diversities in gene proportions, exon–intron structures, and conserved motifs. Genome mapping analysis revealed that HuNAC genes were unevenly scattered on all eleven chromosomes. Synteny analysis indicated that the segmental duplication events played key roles in the expansion of the pitaya NAC gene family. Expression levels of these HuNAC genes were analyzed under cold treatments using qRT-PCR. Four HuNAC genes, i.e., HuNAC7, HuNAC20, HuNAC25, and HuNAC30, were highly induced by cold stress. HuNAC7, HuNAC20, HuNAC25, and HuNAC30 were localized exclusively in the nucleus. HuNAC20, HuNAC25, and HuNAC30 were transcriptional activators while HuNAC7 was a transcriptional repressor. Overexpression of HuNAC20 and HuNAC25 in Arabidopsis thaliana significantly enhanced tolerance to cold stress through decreasing ion leakage, malondialdehyde (MDA), and H2O2 and O2− accumulation, accompanied by upregulating the expression of cold-responsive genes (AtRD29A, AtCOR15A, AtCOR47, and AtKIN1). This study presents comprehensive information on the understanding of the NAC gene family and provides candidate genes to breed new pitaya cultivars with tolerance to cold conditions through genetic transformation.

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Section: Gene Structure and Conserved Motif Analyses Of Hunacssupporting

confidence: 93%

HuNAC20 and HuNAC25, Two Novel NAC Genes from Pitaya, Confer Cold Tolerance in Transgenic Arabidopsis

Xie

Liang

et al. 2022

IJMS

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“…Transcription sequence assembly and normalization of gene expression (fragments per kilobase million, FPKM) were performed using StringTie (v1.3.4, default parameters). RNA-seq data for various abiotic stresses in cultivated peanut were obtained in our previous study, which deposited in the National Center for Biotechnology Information ( http://www.ncbi.nlm.nih.gov/ ) under BioProject PRJNA553073 [ 39 ]. The seedlings of FH1 used for sequencing were cultivated in 1/2 MS medium at room temperature for 10 days and were then treated with 2% NaCl for 24 h, 20% polyethylene glycol (PEG) 6000 for 24 h, low temperature (4 °C) for 24 h, heat (37 °C) for 24 h, 75 μM ABA for 24 h, 1% H 2 O 2 for 24 h. RNA-seq data were analyzed by HISAT2 and Cufflinks to obtain FPKM (fragments per kilobase million) values [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification and expression of SAUR gene family in peanut (Arachis hypogaea L.) and functional identification of AhSAUR3 in drought tolerance

et al. 2022

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Background Small auxin-upregulated RNAs (SAURs) gene family plays important roles in plant growth, development, and stress responses. However, the function of few SAUR genes is known in the peanut (Arachis hypogaea L.), one of the world’s major food legume crops. This study aimed to perform a comprehensive identification of the SAUR gene family from the peanut genome. Results The genome-wide analysis revealed that a total of 162 SAUR genes were identified in the peanut genome. The phylogenetic analysis indicated that the SAUR proteins were classified into eight subfamilies. The SAUR gene family experienced a remarkable expansion after tetraploidization, which contributed to the tandem duplication events first occurring in subgenome A and then segmental duplication events occurring between A and B subgenomes. The expression profiles based on transcriptomic data showed that SAUR genes were dominantly expressed in the leaves, pistils, perianth, and peg tips, and were widely involved in tolerance against abiotic stresses. A total of 18 AhSAUR genes selected from different subfamilies randomly presented 4 major expression patterns according to their expression characteristics in response to indole-3-acetic acid. The members from the same subfamily showed a similar expression pattern. Furthermore, the functional analysis revealed that AhSAUR3 played a negative role in response to drought tolerance. Conclusions This study provided insights into the evolution and function of the SAUR gene family and may serve as a resource for further functional research on AhSAUR genes.

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“…Nearly 7% coding knack of the vascular plants genome is associated with transcriptional factors for regulating target genes at transcriptional level (Rushton et al, 2008). To date, scientists have identified thousands of TFs in plants, however, major TF families (AP2/ERF, HSFs, MYB, WRKY, NAC, etc), mediated through signal transduction pathways, recently been utilized to cope with heat stress in various food crops (Guo et al, 2020;Li et al, 2021;Xiang et al, 2021).…”

Section: Role Of Transcriptional Factors In Response To High Temperature Stressmentioning

confidence: 99%

Response and tolerance mechanism of food crops under high temperature stress: a review

et al. 2022

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High temperature stress events are critical factors inhibiting crop yield. Meanwhile, world population is growing very rapidly and will be reached up to 9 billion by 2050. To feed increasing world population, it is challenging task to increase about 70% global food productions. Food crops have significant contribution toward global food demand and food security. However, consequences from increasing heat stress events are demolishing their abilities to survive and sustain yield when subjected to extreme high temperature stress. Therefore, there is dire need to better understand response and tolerance mechanism of food crops following exposure to heat stress. Here, we aimed to provide recent update on impact of high temperature stress on crop yield of food crops, pollination, pollinators, and novel strategies for improving tolerance of food crop under high temperature stress. Importantly, development of heat-resistant transgenic food crops can grant food security through transformation of superior genes into current germplasm, which are associated with various signaling pathways as well as epigenetic regulation in response to extreme high temperature stress.

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Genome-Wide Identification of NAC Transcription Factors and Their Functional Prediction of Abiotic Stress Response in Peanut

Cited by 32 publications

References 71 publications

HuNAC20 and HuNAC25, Two Novel NAC Genes from Pitaya, Confer Cold Tolerance in Transgenic Arabidopsis

HuNAC20 and HuNAC25, Two Novel NAC Genes from Pitaya, Confer Cold Tolerance in Transgenic Arabidopsis

Genome-wide identification and expression of SAUR gene family in peanut (Arachis hypogaea L.) and functional identification of AhSAUR3 in drought tolerance

Response and tolerance mechanism of food crops under high temperature stress: a review

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