Genome-wide identification of soybean WRKY transcription factors in response to salt stress

WRKY transcription factors play pivotal roles in regulation of stress responses. This study identified 79 WRKY genes in potato (Solanum tuberosum). Based on multiple sequence alignment and phylogenetic relationships, WRKY genes were classified into three major groups. The majority of WRKY genes belonged to Group II (52 StWRKYs), Group III had 14 and Group I consisted of 13. The phylogenetic tree further classified Group II into five sub-groups. All StWRKY genes except StWRKY79 were mapped on potato chromosomes, with eight tandem duplication gene pairs and seven segmental duplication gene pairs found from StWRKY family genes. The expression analysis of 22 StWRKYs showed their differential expression levels under various stress conditions. Cis-element prediction showed that a large number of elements related to drought, heat and salicylic acid were present in the promotor regions of StWRKY genes. The expression analysis indicated that seven StWRKYs seemed to respond to stress (heat, drought and salinity) and salicylic acid treatment. These genes are candidates for abiotic stress signaling for further research.

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“…The motifs were analyzed using the MEME website (http://meme-suite.org/index.html) [4]. In total, 20 motifs were searched per member.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification of the potato WRKY transcription factor family

et al. 2017

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“…These results indicate that, compared to the cultivated potato 22 , S. commersonii possesses a lower number and S. chacoense a higher number of WRKY genes. Both species displayed a number of WRKYs greater than that of barley (45) 46 , castor bean (58) 47 , cucumber (55) 48 , rapeseed (43) 49 and grapevine (59) 50 , and lower than that of cotton (120) 51 , maize (136) 52 , soybean (131) 53 and rice (100) 25 . From this comparison, it appears that the number of WRKY encoding genes is not proportional to the genome size of the respective plant species, as also reported by Waqas et al 54 .…”

Section: Structural Analysis Of Scwrkys and Schwrkys Revealed Interspmentioning

confidence: 99%

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Villano

Esposito

D’Amelia

et al. 2020

Sci Rep

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Wild potatoes, as dynamic resource adapted to various environmental conditions, represent a powerful and informative reservoir of genes useful for breeding efforts. WRKY transcription factors (TFs) are encoded by one of the largest families in plants and are involved in several biological processes such as growth and development, signal transduction, and plant defence against stress. In this study, 79 and 84 genes encoding putative WRKY TFs have been identified in two wild potato relatives, Solanum commersonii and S. chacoense. Phylogenetic analysis of WRKY proteins divided ScWRKYs and SchWRKYs into three Groups and seven subGroups. Structural and phylogenetic comparative analyses suggested an interspecific variability of WRKYs. Analysis of gene expression profiles in different tissues and under various stresses allowed to select ScWRKY045 as a good candidate in woundingresponse, ScWRKY055 as a bacterial infection triggered WRKY and ScWRKY023 as a multiple stressresponsive WRKY gene. Those WRKYs were further studied through interactome analysis allowing the identification of potential co-expression relationships between ScWRKYs/SchWRKYs and genes of various pathways. Overall, this study enabled the discrimination of WRKY genes that could be considered as potential candidates in both breeding programs and functional studies.Plants experience environmental constrains and pathogen attacks during their life. Being sessile organisms, their survival depends on the ability to properly and promptly reprogram cellular networks. Several and different classes of transcription factors (TFs) work as "master regulators" and "selector genes", being able to control processes that specify cell types and developmental patterning and modulate specific pathways. Among them, WRKY factors are drawing a great deal of interest in the scientific community due to their ability to simultaneously cope with multiple stresses 1,2 . They are notorious for coordinating signals in plant immunity response against several pathogens and pest attacks 3,4 . More recently, it has been confirmed that WRKYs also base defence mechanism to abiotic stresses and play a key role in cross-talk pathway networks between plant response and development 5,6 . Their involvement into multiple stress response and in plant growth regulation is evidenced by their W-box specific DNA binding 7,8 . Besides, WRKY binds sugar responsive elements and, very recently, it has been demonstrated that they activate sugar responsive genes through an epigenetic mechanism of control 9 . The systematic classification of components of the WRKY family is well organized. It is based on the WRKY binding domain (WD) characteristics along with those of the Zinc Finger (ZF) motif, which is typically present downstream the WD. WD consists of 60 amino acids structured as four-stranded β-sheets able to enter the major groove of B-form DNA. The highly conserved motif is "WRKYGQK". According to the number of WDs and the type of zinc finger motif, WRKY proteins can be classified into three Groups,...

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“…Recent research has demonstrated that WRKY genes are involved in responses to various stresses and there is now compelling evidence that WRKYs are plant transcription factors that regulate tolerance to abiotic stresses [48]. Gene expression studies have shown that 20 AtWRKY genes in Arabidopsis, 41 OsWRKY genes in rice, 66 GmWRKY genes in soybean, 41 BrWRKY genes in Brassica rapa, and 74 BnWRKY genes in rapeseed are involved in responses to abiotic stresses [14,18,34,[49][50][51]. In this study, 44 SiWRKY genes were expressed differentially in response to waterlogging and drought stresses, indicating that these genes may also be involved in responses to abiotic stresses.…”

Section: Identification Of Siwrky Genes Involved In Responses To Abiomentioning

confidence: 99%

Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses

Liu

et al. 2017

BMC Plant Biol

111

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Background: Sesame (Sesamum indicum L.) is one of the world's most important oil crops. However, it is susceptible to abiotic stresses in general, and to waterlogging and drought stresses in particular. The molecular mechanisms of abiotic stress tolerance in sesame have not yet been elucidated. The WRKY domain transcription factors play significant roles in plant growth, development, and responses to stresses. However, little is known about the number, location, structure, molecular phylogenetics, and expression of the WRKY genes in sesame.

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Genome-wide identification of soybean WRKY transcription factors in response to salt stress

Cited by 76 publications

References 75 publications

Genome-wide identification of the potato WRKY transcription factor family

Genome-wide identification of the potato WRKY transcription factor family

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses

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