Genome-wide identification of the aspartic protease gene family and their response under powdery mildew stress in wheat

Yang, Yanlin; Feng, Deshun

doi:10.1007/s11033-020-05948-9

Cited by 11 publications

(4 citation statements)

References 55 publications

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“…Screening of a yeast two hybrid library and coimmunoprecipitation in this study revealed aspartic protease 1 (AP1) orthologs from Arabidopsis and cotton interact with VdCE11. Aspartic proteases, which are widely distributed in various tissues and organs of plants and animals, are involved in protein processing or degradation, senescence, stress response, programmed cell death, and reproduction ( 48 , 49 ). Some studies revealed the involvement of aspartic protease in plant immunity.…”

Section: Discussionmentioning

confidence: 99%

Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton

Qin

Huang

et al. 2023

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton

Qin

Huang

et al. 2023

Microbiol Spectr

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“…The peroxisome, an essential organelle of plants, plays a key role in redox signal transduction and lipid homeostasis [11][12][13][14][15]. APs are mainly involved in such processes as metabolism, precursor protein processing, protein degradation, and programmed cell death, as well as pollen germination and pollen-tube growth in plants [16][17][18]. D-7 LEA is a stress-responding protein that massively accumulates in the late embryonic stage and is generated under adverse conditions such as drought.…”

Section: Discussionmentioning

confidence: 99%

Proteomic study of differentially expressed proteins in seeds between parents and offspring of castor bean (Ricinus communis L.)

Liang

Luo

Zhang

et al. 2022

Preprint

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Castor bean (Ricinus communis L.), one of the top 10 oilseed crops in the world, has high economic value. Hybridization is the most direct and effective method to breed new varieties with high yield, high oil content, and strong stress resistance. Therefore, prediction of desired traits in castor hybrid offspring is particularly important. In this study, proteomic analysis was performed to identify differentially expressed proteins (DEPs) in seeds between castor hybrid offspring and their female (Lm female line aLmAB2) and male parents (CSR•181). Among the DEPs upregulated in the seeds of hybrid offspring, the majority were related to seed yield and stress resistance, while some were related to oil synthesis and fatty acid synthesis and metabolism in seeds. In other words, the hybrid offspring showed heterosis for seed yield, stress resistance, oil synthesis, and fatty acid synthesis and metabolism when compared with their parents. Further, real-time quantitative polymerase chain reaction assays were performed on 12 genes encoding DEPs involved in oil synthesis, pollen abortion, yield, and stress resistance of seeds. The results showed that the expression levels of the 12 genes were consistent with those of the DEPs.

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“…Through multiple sequence comparisons, we found that the TiAP1 gene is 98% similar to scaffold 208 assembled from the T . intermedium genome (https://jgi.doe.gov/), and Yang and Feng (2020) analysed the aspartic protease gene family and their response under powdery mildew stress in wheat. Among those family genes, the AP gene which was most similar to TiAP1 , TRIAE_CS42_5DS_TGACv1_456657_AA1475640.1, was located on the chromosome 5DS with 70% identity.…”

Section: Discussionmentioning

confidence: 99%

Thinopyrum intermedium TiAP1 interacts with a chitin deacetylase from Blumeria graminis f. sp. tritici and increases the resistance to Bgt in wheat

Yang

Fan

Liu

et al. 2021

Plant Biotechnology Journal

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Summary The biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt) is a crucial factor causing reduction in global wheat production. Wild wheat relatives, for example Thinopyrum intermedium, is one of the wild‐used parents in wheat disease‐resistant breeding. From T. intermedium line, we identified the aspartic protease gene, TiAP1, which is involved in resistance against Bgt. TiAP1 is a secreted protein that accumulates in large amounts at the infection sites of Bgt and extends to the intercellular space. Yeast two‐hybrid, luciferase complementation imaging and bimolecular florescent complimentary analysis showed that TiAP1 interacted with the chitin deacetylase (BgtCDA1) of Bgt. The yeast expression, purification and in vitro test confirmed the chitin deacetylase activity of BgtCDA1. The bombardment and VIGS‐mediated host‐induced gene silencing showed that BgtCDA1 promotes the invasion of Bgt. Transcriptome analysis showed the cell wall xylan metabolism, lignin biosynthesis‐related and defence genes involved in the signal transduction were up‐regulated in the transgenic TiAP1 wheat induced by Bgt. The TiAP1 in wheat may inactivate the deacetylation function of BgtCDA1, cause chitin oligomers expose to wheat chitin receptor, then trigger the wheat immune response to inhibit the growth and penetration of Bgt, and thereby enhance the resistance of wheat to pathogens.

show abstract

Genome-wide identification of the aspartic protease gene family and their response under powdery mildew stress in wheat

Cited by 11 publications

References 55 publications

Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton

Verticillium dahliae Effector VdCE11 Contributes to Virulence by Promoting Accumulation and Activity of the Aspartic Protease GhAP1 from Cotton

Proteomic study of differentially expressed proteins in seeds between parents and offspring of castor bean (Ricinus communis L.)

Thinopyrum intermedium TiAP1 interacts with a chitin deacetylase from Blumeria graminis f. sp. tritici and increases the resistance to Bgt in wheat

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