Genome-wide location analysis reveals a role for Sub1 in RNA polymerase III transcription

Abstract

Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a system reconstituted with all recombinant factors. Sub1 was required for optimal Pol III gene transcription in exponentially growing cells.

Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA

“…Sub1 interacts with RNA polymerase II 24,25 and III 26 and plays an essential regulatory role in transcription initiation, elongation, and mRNA 3’-end formation 24,27,28 . We therefore tested whether Sub1 can bind to a G4 region on chromosomal DNA in vivo .…”

Synonymous codons, ribosome speed, and eukaryotic gene expression regulation

“…In addition to the transcription factors, PolIII activity is controlled by global regulators, including Maf1, a negative regulator [13], and Sub1, a stimulator of PolIII activity [14]. In contrast to this global regulation, differential regulation of transcription between tRNA genes is generally not well documented.…”

RNA polymerase III transcription control elements: Themes and variations

“…Since then, the sharing of components by the three transcription systems, or the presence of paralogous polypeptides playing similar functions in different systems, has perhaps become less surprising, but not less challenging in terms of mechanistic interpretation (Carter and Drouin, 2010;Geiger et al, 2010;Kassavetis et al, 2010;Lefevre et al, 2011;Teichmann et al, 2010). As significant examples, we mention in particular the Pol II elongation factor TFIIS and the Pol II coactivator protein Sub1, that have recently been located at Pol IIItranscribed genes in S. cerevisiae by genome-wide studies, but whose function in transcription of these genes is only partially understood (Ghavi-Helm et al, 2008;Rosonina et al, 2009;Tavenet et al, 2009). Along the same vein, the accumulation of genome-wide location analyses of Pol II transcriptional regulators has recently led to the notion that the participation of Pol II factors in Pol III transcription (and, consequently, the occurrence of cis-regulatory elements acting as Pol III transcription enhancers) might be widespread (Noma and Kamakaka, 2010;Venters et al, 2011).…”