Genome-wide search for microsatellite markers associated with radiologic alterations in the navicular bone of Hanoverian warmblood horses

Abstract

The aim of this study was to identify quantitative trait loci (QTLs) for pathologic changes in the navicular bone in Hanoverian warmblood horses. Seventeen paternal half-sib groups comprising 192 individuals were analyzed in a whole-genome scan. These families included 144 progeny and grandchildren, which were randomly chosen from the Hanoverian warmblood. Three different traits were considered: deformed canales sesamoidales and radiographic changes in the contour and in the structure of the navicular bone. The genome scan included in total 214 highly polymorphic microsatellite markers. The putatively linked genomic regions on equine chromosomes (ECA) 2, 3, 10, and 15 were refined using 53 additional microsatellites. Chromosome-wide significant QTLs were located on five different equine chromosomes (ECA2, 3, 4, 10, and 26). Genome-wide significant QTLs were on ECA2 at 48 cM and on ECA10 from 45.5 to 49.8 cM. This study was a first step to get more insight into the molecular genetic determination of radiologic changes in the equine navicular bone.

Refinement of quantitative trait loci on equine chromosome 10 for radiological signs of navicular disease in Hanoverian warmblood horses

“…1986; Dik & van den Broek 1995; Stock & Distl 2006). Heritability estimates varied from 0.10 to 0.31 in threshold models (Diesterbeck et al. 2007).…”

Fine mapping a quantitative trait locus on horse chromosome 2 associated with radiological signs of navicular disease in Hanoverian warmblood horses

“…1986; Dik & van den Broek 1995; Philipsson et al. 1998; Stock & Distl 2006) and recently, a quantitative trait locus (QTL) for pathological alterations in the contour of the navicular bone (RAC) at 48 ( UCDEQ380 ) to 62.2 cM ( TKY340 ) on horse chromosome (ECA) 2 was reported by Diesterbeck et al. (2007).…”

“…Radiological appearance of the navicular bones was classified according to the evaluation scheme of Brunken (1986) (Table S1). A more detailed description of the pedigree structure and phenotypic traits for radiological alterations of the navicular bone can be found elsewhere (Diesterbeck et al. 2007).…”

“…These 58 polymorphic microsatellites covered the entire horse chromosome 2 evenly at an average spacing of 2.1 Mb. Genotyping was performed according to Diesterbeck et al. (2007)…”

“…The region of ECA2 between 28.43 and 47.92 Mb is syntenic to Homo sapiens chromosome 1p35–p36. In this region, there are genes linked to human osteoarthritis, which resembles navicular disease (Diesterbeck et al. 2007).…”