Genome-wide transcriptional analysis of temperature shift in L. interrogans serovar lai strain 56601

Abstract

Background: Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptation to a range of new environmental conditions in the organs and tissues of the infected host. Several studies have shown that a shift in culture temperature from 28°C to 37°C, similar to that encountered during infection of a host from an environmental source, is associated with differential synthesis of several proteins of the outer membrane, periplasm and cytoplasm. The whole genome of the Leptospira interrogans serogroup Icterohaemorrhagiae serovar lai type strain #56601 was sequenced in 2003 and microarrays were constructed to compare differential transcription of the whole genome at 37°C and 28°C.

Cellular mechanism for spontaneous calcium oscillations in astrocytes1

“…However, the activation of astrocytes can also affect neurons [6] , via releasing varieties of neurotransmitters, including glutamate and ATP [7,8] . Although the mechanism of neurotransmitter secreting is unclear, this process is believed to couple with intracellular Ca 2+ elevation [9,10] . Ca 2+ is one of the most important second messengers and is thought to mediate communication between neurons and astrocytes [3,11] .…”

Multiple Posttranslational Modifications of Leptospira biflexa Proteins as Revealed by Proteomic Analysis

“…No large-scale microarray data or extensive proteomic analyses have been reported for the saprophyte, although Thongboonkerd and colleagues compared total protein lysate of L. biflexa to multiple pathogenic strains to identify potential virulence factors (10). In contrast, extensive microarray and proteomic studies have been performed on various host-associated species (11)(12)(13)(14)(15)(16)(17)(18)(19)(20). These limitations in our basic knowledge of L. biflexa reduce its utility as a model organism, since there are no known protein markers for the outer membrane and no abundantly synthesized proteins have been reported.…”

Re-characterization of an extrachromosomal circular plasmid in the pathogenic <italic>Leptospira interrogans</italic> serovar Lai strain 56601

“…The genomic sequence of Leptospira interrogans serovar Lai strain 56601 (str.56601) within the genus Leptospira was first finished in 2003 using classical shotgun genome sequencing. Subsequent transcriptomic and proteomic data from L. interrogans has further deepened our understanding of this strain [9][10][11][12][13] and facilitated the study of pathogenesis in Leptospirosis. However, some assembly errors that were caused by an abundance of repeat sequences that globally distributed in the genome have been found and finally corrected in str.56601 [9,14].…”