Genome-wide transcriptional profiling reveals molecular signatures of secondary xylem differentiation in Populus tomentosa

Yang, Xiaohui; Li, X G; Li, B L; Zhang, D Q

doi:10.4238/2014.november.11.14

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…High levels of PbCCOMT1 and PbCCOMT3 expression in stems is consistent with the expression of PtCCOMT in Populus tomentosa. High levels of CCOMT expression is associated with xylem development in woody plants (Shi et al, 2010;Yang et al, 2014). At present, many studies have used genetic engineering to suppress CCOMT expression to downregulate lignin (Wagner et al, 2011;Li et al, 2013;Pang et al, 2014;Zhang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. With high nutritional value in its fruits, Dangshan Su pear has been widely cultivated in China. The stone cell content in fruits is a key factor affecting fruit quality in pear, and the formation of stone cells has been associated with lignin biosynthesis. O-Methyltransferase (OMT) is a key enzyme involved in lignin metabolism within the phenylpropanoid pathway. Here, we screened 26 OMT genes from the Pyrus bretschneideri cv. Dangshan Su genome using the DNATOOLs software. To characterize the OMT gene family in pear, gene structure, chromosomal localization, and conserved motifs of PbOMTs were analyzed. PbOMTs were divided into two categories, type I (designated PbCCOMTs) and type II (designated PbCOMTs), indicating the differentiation of function during evolution. Based on the analysis of multiple sequence alignment, cis-element prediction, and phylogenetic relationships, two candidate genes, PbCCOMT1 and PbCCOMT3, were selected for the analysis of temporal and spatial gene expression in pear. The promoter regions of both PbCCOMT1 and PbCCOMT3 contain regulatory motifs for lignin synthesis. Moreover, the two genes show high similarity and close phylogenetic relationships with CCOMTs in other species. Expression analysis showed that transcript levels of two PbCCOMTs were positively associated with the contents of both stone cells and lignin during the development of pear fruit. These results suggest that PbCCOMT1 and PbCCOMT3 are closely associated with lignin biosynthesis. These findings will help clarify the function of PbOMTs in lignin metabolism and to elucidate the mechanisms underlying stone cell formation in pear.

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Section: Discussionmentioning

confidence: 99%

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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“…One of the upregulated genes was SAUR-like auxin-responsive protein is a hormone-related gene ( Table 1 ). By transcriptome analysis, Yang et al [ 33 ] found that SAUR-like is related to xylem differentiation processes and regulating genes involved in cell division processes. Additional experiments will be required to analyze which of these events are involved during stoma development.…”

Section: Discussionmentioning

confidence: 99%

LEA13 and LEA30 Are Involved in Tolerance to Water Stress and Stomata Density in Arabidopsis thaliana

López-Cordova

Ramírez-Medina

Silva-Martínez

et al. 2021

Plants

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Late embryogenesis abundant (LEA) proteins are a large protein family that mainly function in protecting cells from abiotic stress, but these proteins are also involved in regulating plant growth and development. In this study, we performed a functional analysis of LEA13 and LEA30 from Arabidopsis thaliana. The results showed that the expression of both genes increased when plants were subjected to drought-stressed conditions. The insertional lines lea13 and lea30 were identified for each gene, and both had a T-DNA element in the regulatory region, which caused the genes to be downregulated. Moreover, lea13 and lea30 were more sensitive to drought stress due to their higher transpiration and stomatal spacing. Microarray analysis of the lea13 background showed that genes involved in hormone signaling, stomatal development, and abiotic stress responses were misregulated. Our results showed that LEA proteins are involved in drought tolerance and participate in stomatal density.

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“…[78]. In A C C E P T E D M A N U S C R I P T 21 addition, basic proline-rich protein 1 may represent a protein linking microtubule regulation with cell wall biogenesis [79].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Integrating cell biology and proteomic approaches in plants

Takáč

Šamajová

Šamaj

2017

Journal of Proteomics

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Genome-wide transcriptional profiling reveals molecular signatures of secondary xylem differentiation in Populus tomentosa

Cited by 8 publications

References 35 publications

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

LEA13 and LEA30 Are Involved in Tolerance to Water Stress and Stomata Density in Arabidopsis thaliana

Integrating cell biology and proteomic approaches in plants

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