Genomic Analysis of Fluoroquinolone- and Tetracycline-Resistant <i>Campylobacter jejuni</i> Sequence Type 6964 in Humans and Poultry, New Zealand, 2014–2016

French, Nigel P.; Zhang, Ji; Carter, Glen P.; Midwinter, Anne C.; Biggs, Patrick J.; Dyet, Kristin; Gilpin, Brent; Ingle, Danielle J.; Mulqueen, Kerry; Rogers, Lynn; Wilkinson, David A.; Greening, Sabrina S.; Muellner, Petra; Fayaz, Ahmed; Williamson, Deborah A.

doi:10.3201/eid2512.190267

Cited by 31 publications

(21 citation statements)

References 38 publications

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“…The fexA gene were related to phenicol exporter was presented in two C. coli isolates ST830 (33,36). The tetracycline resistance gene tetO was reported from the previous study which were located on plasmid causing the spread of tetO in C. jejuni (33,37). In addition, tetO was also observed in C. jejuni isolates from wild bird and humans (22).…”

Section: Discussionmentioning

confidence: 88%

Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China

et al. 2020

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Campylobacter is one of the most important foodborne pathogens worldwide, and poultry is regarded as the main reservoir of Campylobacter. The contamination of Campylobacter in broiler chickens at the farm level is closely related to the transmission of Campylobacter in the poultry production chain. This study identified 464 Campylobacter isolates from 1,534 samples from broiler rearing period and slaughtering process including 233 Campylobacter jejuni isolates and 231 Campylobacter coli isolates. We have observed a dynamic distribution of Campylobacter during broiler chicken production, that 66.3% of Campylobacter isolates were C. jejuni during broiler rearing period, while C. coli occupied 60.4% of Campylobacter isolates during the broiler slaughtering process. A tag-label method allowed us to track the dynamic of Campylobacter in each broiler chicken from 31-day age at rearing to the partition step in the slaughterhouse. At the 31-day during rearing, 150 broiler chicken were labeled, and was tracked for Campylobacter positive from rearing period to slaughtering process. Among the labeled broiler, 11 of the tracking broiler samples were able to detect Campylobacter from rearing period to slaughtering. All Campylobacter isolates from the 11 tracking samples were sequenced and analyzed. C. jejuni isolates were divided into four STs and C. coli isolates were divided into six STs. Isolates with identical core genome were observed from the same tag-labeled samples at different stages indicating a vertical transmission of Campylobacter in the early broiler meat production. Meanwhile, the core genome analysis elucidated the cross-contamination of Campylobacter during the rearing period and the slaughtering process. The virulotyping analysis revealed that all C. jejuni isolates shared the same virulotypes, while C. coli isolates were divided into three different virulotypes. The antimicrobial resistance gene analysis demonstrated that all Campylobacter isolates contained at least two antibiotic resistance genes (ARGs), Tang et al. Campylobacter From Broiler in China and the ARG profiles were well-corresponding to each ST type. Our study observed a high prevalence of Campylobacter during the early chicken meat production, and further studies will be needed to investigate the diversity and transmission of Campylobacter in the poultry production chain.

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Section: Discussionmentioning

confidence: 88%

Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China

et al. 2020

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“…Today, there are more than 60 tetracycline resistance genes described and besides the already mentioned mechanisms of action, resistance mediated by enzymatic inactivation of the molecule was also reported [7,[10][11][12][13]. The improvement of diagnostic techniques and the elimination of false positives corroborate the knowledge that the tet(O) sequence is the only resistance determinant to tetracycline in C. jejuni and C. coli [4,14], especially associated with the acquisitions of plasmids containing the gene [15][16][17], although it can also be found in the chromosome [18][19][20]. Mosaic genes are common amongst genes responsible for ribosomal protection proteins and several of these elements have been described, with the majority deriving from tet(O), tet(W) and tet32, including tet(O/32/O) recently found in C. coli and C. jejuni isolated from humans [7].…”

Section: Introductionmentioning

confidence: 88%

Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

et al. 2020

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Campylobacter is one of the most important microorganisms responsible for foodborne diseases in the EU. In this study, we investigated resistance to tetracycline in 139 Campylobacter jejuni and Campylobacter coli samples isolated from human clinical cases. From these, 110 were resistant to tetracycline, with MIC (minimal inhibitory concentration) varying in a range of 1 to >512 μg/mL, and 109 (78.4%) carried tet(O), a gene that confers resistance to tetracycline through the expression of a protein that confers protection to the ribosome. Amongst the tetracycline-resistant isolates, one C. jejuni (HCC30) was the only tet(O)-negative sample, presenting an MIC of 256 μg/mL. Instead, the mosaic gene tet(O/M/O) was found in HCC30 and, as far as we know, this is the first description of this chimeric gene originating from homologous recombination between tet(O) and tet(M). The previously described mosaic gene tet(O/32/O), also found in Campylobacter, presents a chimeric structure very similar to that of tet(O/M/O), affecting domains II and III of encoded proteins distantly related to the elongation factor G (EF-G). The tet(O/M/O) mosaic gene has been found in nucleotide databases in several genomes of Campylobacter isolated from different origins, indicating its frequent acquisition, even though it can be undetected through screening by PCR with specific tet(O) primers. In this work, we address the improvement of classical PCR to efficiently diagnose the most prevalent tetracycline resistance determinants in Campylobacter, including tet(O/M/O), which should be taken into account in the optimization of campylobacteriosis treatments.

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“…In addition, STs strongly associated with New Zealand, and found rarely elsewhere, were common in this dataset, including Table 4). 4,22…”

Section: Whole Genome Sequencing and C Jejuni And C Coli Populationmentioning

confidence: 99%

Source attributed case-control study of campylobacteriosis in New Zealand

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Campbell

Hathaway

et al. 2021

International Journal of Infectious Diseases

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Genomic Analysis of Fluoroquinolone- and Tetracycline-Resistant Campylobacter jejuni Sequence Type 6964 in Humans and Poultry, New Zealand, 2014–2016

Cited by 31 publications

References 38 publications

Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China

Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China

Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

Source attributed case-control study of campylobacteriosis in New Zealand

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