Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

Abstract

Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) causing great economic losses, since it first outbreak in southeast China in 2009. Vibrio parahaemolyticus , carrying the pir A and pir B toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp ( Peneaus monodon ) of south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh , AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of total 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type three secretion system-1 (TTSS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pir A and pir B. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline, macrolide and cephalosporin groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic resistance V. parahaemolyticus isolates between production sites, and the identification of candidate strains for production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

Complete genome sequence of highly pathogenic Vibrio parahaemolyticus isolated from mariculture Penaeus vannamei reveals virulence factor genes

“…In the genome data, no TDH or TRH gene was identified, but a gene encoding TLH was identified. This result is consistent with the results reported by other scholars (Ahmmed et al., 2019). This finding indicates that seafood is rarely toxic to humans when heated adequately.…”

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

“…Other studies also suggested that genes involved in chemotaxis and motility, Type III secretion system‐1 (T3SS‐1) and its secreted effectors, thermolabile haemolysin were potentially contributing to the shrimp infection (Ahmmed et al . 2019; Shaheer et al . 2019; Lu et al .…”

Outbreak of COVID-19: Impact on Socio-Economic Condition of Shrimp Farmers in South-West Coastal Bangladesh

“…Moreover, Shrimp is also prone to various disease outbreaks like WSSV, AHPND and various pathogenic organisms as the farmers were not able to maintain their farms properly during this pandemic [30]. Therefore, due to COVID-19 the Shrimp farmers harvested the diseased Shrimp and sold at lower price that ultimately affect their income status.…”