Genomic and Haplotype Comparison of Butanol Producing Bacteria Based on 16S rDNA

Abstract

High butanol demand for transportation fuel triggers butanol production development. Exploration of butanolproducing bacteria using genomic comparison and biogeography will help to develop butanol industry. The objectives of this research were butanol production, genome comparison and haplotype analysis of butanolproducing bacteria from Ranu Pani Lake sediment using 16S rDNA sequences. The highest butanol concentrations were showed by Paenibacillus polymyxa RP 2.2 isolate (10.34 g.L -1 ), followed by Bacillus methylotrophicus RP 3.2 and B. methylotrophicus RP 7.2 isolate (10.11 g.L -1 and 9.63 g.L -1 ) respectively. Paenibacillus polymyxa RP 2.2 showed similarity in nucleotide composition (ATGC) with B. methylotrophicus RP 3.2, B. methylotrophicus RP 7.2, P. polymyxa CR1, Bacillus amyloliquefaciens NELB-12, and Paenibacillus polymyxa WR-2. Clostridium acetobutylicum ATCC 824 showed similarity in nucleotide composition (ATGC) with Clostridium saccharoperbutylacetonicum N1-4, and Clostridium saccharobutylicum Ox29. The lowest G+C content was C. saccharobutylicum Ox29 (51.35%), and the highest was B. methylotrophicus RP 7.2 (55.33%). Conserved region of 16S rDNA (1044 bp) were consisted of 17 conserved sequences. The number of Parsimony Informative Site (PIS) was 319 spot and single tone was 48 spot. We found in this study that all of butanolproducing bacterial DNA sequences have clustered to 8 haplotypes. Based on the origin of sample, there were three haplotype groups. Bacteria from group A were could produce butanol 8.9-10.34 g.L -1 , group B 9.2-14.2 g.L -1 and group C was could produce butanol 0.47 g.L -1 . The haplotype analysis of bacteria based on 16S rDNA sequences in this study could predict capability of butanol production.